The invention discloses a half cutting machine of a culture medium for experiments. The half cutting machine structurally comprises a half cutting structure, a control box, a belt, a motor, a bottom rack and a conveying platform. The bottom of the conveying platform is movably provided with the bottom rack, one end of the conveying platform is movably provided with the half cutting structure and the control box, one side of the control box is provided with the belt, and one end of the belt is mechanically connected with the conveying platform. According to the half cutting machine, a first clamping sheet, second clamping sheets and a third clamping sheet form a triangular structure, the surfaces of the first clamping sheet, the second clamping sheets and the third clamping sheet are smooth, the culture medium is half wrapped for fixing and prevented from relatively sliding, the globular or original culture medium is halved, and a cutter directly upwards cuts off the culture medium fromthe bottom and directly cuts the culture medium into halves. According to the half cutting machine, the first clamping sheet, the second clamping sheets and the third clamping sheet are semi-circulararc plates, the head and tail ends are slightly bent to fix the culture medium, the cutter upwards cuts the culture medium from the bottom, the culture medium is not prone to moving outwards, and thecut culture medium is more precise.