A special-effect biological enzyme formaldehyde-removing odor-removing agent and a preparing method thereof are disclosed. The agent includes, by weight, 2-6% of laccase, 0.5-2% of formaldehyde dehydrogenase, 0.5-2% of formate dehydrogenase, 2-5% of pectase, 0.1-0.2% of cis-benzene dihydrodiol dehydrogenase, 0.1-0.5% of epoxide hydrolase, 0.1-0.5% of NADH, 0.5-1.5% of an amino compound, 0.01-0.05%of an activator, 1-2% of a stabilizer and 10-15% of a plant extractant, with the balance being water. The formaldehyde-removing odor-removing agent is safe, environmentally friendly and convenient tooperate. Furniture, wallpaper, leather, carpets, floor, curtains, and other house decoration substrates can be sprayed with the formaldehyde-removing odor-removing agent directly, and formaldehyde, amino, TVOC and other pollution gas in air can be decomposed.