49 results about "Abortions rates" patented technology

The invention provides application of decidua NK cells and decidua NK cell exosomes from cell subsets in preparing drugs and auxiliary therapeutic agents for treating diseases related to infertility.Experiments prove that the exosomes treat endometrium growth disorders by promoting endometrial thickness increasing, improving endometrial cell activity, reducing endometrial cell damage, promoting VEGF expression, maintaining stem features of endometrium matrix cells and stimulating proliferation, so that the pregnancy success rate of endometrium damage model mice is increased to 50-70% from 20%; and the exosomes treat diseases related to maternal-fetal immunologic tolerance disorders by playing a role of immunologic tolerance, reducing a spontaneous abortion rate and improving a help T lymphocyte level. Besides, the exosomes can effectively increase the developmental rate of zygotes fertilized no matter in vitro or in vivo in a multiple mode, meanwhile, an in-vitro fertilization rate, an implantation rate of implantation and a birth rate are also increased, and positive assisting effects on infertility treatment are achieved.

The invention relates to a Chinese herbal medicine additive for preventing abortion of sows and a pig feed containing the Chinese herbal medicine additive, belonging to the technical field of medicines. The additive comprises the following components in parts by weight: 30-90 parts of codonopsis pilosula, 10-30 parts of largehead atractylodes rhizome, 30-90 parts of poria, 10-30 parts of himalayan teasel root, 10-30 parts of mistletoe, 10-30 parts of semen cuscutae, 6-18 parts of donkey-hide gelatin, 10-30 parts of fried folium artemisiae argyi, 6-18 parts of fructus amomi villosi, 10-30 parts of perilla stem, 10-30 parts of parched eucommia, 10-30 parts of morinda officinalis, 15-45 parts of prepared rehmannia root, 15-45 parts of angelica sinensis, 10-30 parts of fragant peony, 10-30 parts of fried scutellaria baicalensis, 10-30 parts of fried cortex phellodendri, 30-90 parts of parched medicated leaven, 30-90 parts of parched hawthorn fruits, 30-90 parts of calcined os draconis, 30-90 parts of calcined oyster and 6-18 parts of liquorice. According to the Chinese herbal medicine additive, the constitution of pregnant sows can be obviously improved, the resistances to various pathogenic factors are enhanced, and the abortion rate of the pregnant sows is well reduced.

The invention relates to the technical field of animal husbandry breeding, in particular to a feeding management method for a sow in a gestation period. The method comprises the steps of building a pig house, disinfecting the sow, feeding the sow and the like. The feeding feed is mainly prepared from wheat bran, bean pulp, corn flour, sweet potato powder, peanut powder, bone meal, shell powder, egg shell powder, fish meal, shrimp meal, animal livers, sunflower seed powder, pumpkin seed powder, yam flour, porphyra, soybean sprouts, green tea, potato powder, a premix compound, rock flour, calcium hydrophosphate, lemon peels, plant extracts and the like. The plant extracts are mainly prepared from codonopsis pilosula, taxillus sutchuenensis, radix dipsaci, astragalus mongholicus, semen cuscutae, scutellaria baicalensis, ramie root, purple perilla, fructus amomi, bighead atractylodes rhizome, folium artemisiae argyi, caulis bambusae in taeniam, ligusticum wallichii, radix glycyrrhiza and the like. According to the feeding management method, the feeding feed has abundant nutrition and energy, no poison and no harm, and the functions such as benefiting qi and nourishing blood, strengthening kidney for fetus soothing and preventing abortion; the average abortion rate of the sow in the gestation period is effectively lowered and the average farrowing rate of the sow in the gestation period is effectively increased, and the benefit of pig feeding is improved.

The invention discloses a chemical regulating method for keeping a sterile line of thermo-sensitive genic male sterile wheat BNS thoroughly aborted. Starting from the stamen development of the sterile line of the thermo-sensitive genic male sterile wheat BNS to the pistil-stamen primordium differentiation period until wheat ears are completely extracted from leaf sheaths of flag leaves, plants of the sterile line of the thermo-sensitive genic male sterile wheat BNS are subjected to whole-plant spraying treatment by adopting a 500U/L catalase solution for one time every four days from the first day, wherein the spraying quantity is 50-80L/mu, and other plants refer to an ordinary field cultivation management mode. Through field cultivation, the 500U/L catalase solution is adopted for treatment, so that the pollen abortion rate of the BNS sterile line is approximate to the complete sterility level, the self-fruitful rate is 0, and the requirement on the sterile line in the production of hybrid wheat is met. The chemical regulating method is relatively simple in operation, lower in investment cost, time-saving and labor-saving, and has a broad application prospect.

The invention discloses a cloning method of the animal somatic cells. The cloning method of the animal somatic cells is that tetraploid blastomere in 8-16 cell stage which is obtained from vitro fertilization is transferred into a somatic cloned embryo in 8-16 cell stage to get a compound embryo; the compound embryo is cultured to get a cloned animal. The invention initiatively changes the sourceof trophoblast cell; the tetraploid blastomere obtained from vitro fertilization is put into the somatic cloned embryo in 8-16 cell stage, which can be developed into trophoblast cell; then the placenta quality is improved, the development condition for the fetus is promoted, the pregnancy rate of the cloned embryo is increased, the abortion rate and the dystocia rate is decreased and the cloningefficiency of the somatic cell is greatly improved. As the fetus is constituted by ploid cells, the tetraploid blastomere can only develop into the placenta without the possibility of developing intofetus. Therefore, the method does not affect the individual cloned animal.

The present invention discloses a method for auxiliary identification of a corn haploid induction line. The method comprises the following steps: detecting a seed setting rate, an endosperm abortion rate and an embryo abortion rate of corn requiring detection, wherein the corn candidate requiring detection is determined as the corn haploid induction line if the seed setting rate, the endosperm abortion rate and the embryo abortion rate of the corn requiring detection meet the following conditions such as 1), 2) or 3). The method is particularly suitable for cultivation of a haploid induction line capable of carrying out haploid induction in a target area ??environment, wherein corn suitable for growing in the target area is adopted as a female parent, a known haploid induction line is adopted as a male parent, and operations are performed according to the method, such that the haploid induction line having a haploid induction character of the male parent and a target area ??environment adaptability character of the female parent can be obtained.

The invention discloses a seed selection method for cold resistant and bolting resistant brassica oleracea var. According to the invention, original cabbage excellent self-incompatible line and (male parent) and other cabbages (fertile, female parent) are subjected to hybridization through a castration florescence, progeny is subjected to backcrossing fourth generation through the emasculation florescence, fine spraying is carried out on stigma by warm water with sodium bicarbonate having concentration being 1% at the temperature of 28-32 DEG C at florescence, the substances of the stigma such as callose is broken up, so that self-incompatibility is changed to self-compatibility, and the free pollination by honeybees among the plants at the florescence finally. On one hand, the original parent enables purification and renovation, on the other hand, through multi-generation florescence pollination, the self-incompatibility is reduced, the self-compatibility is enhanced, self-incompatibility is gradually changed to self-compatibility, for the utilization of breeding of a cytoplasmic male sterile line, the breeding efficiency is increased, the disadvantages of self incompatibility and artificial bud pollination required by line breeding can be overcome, at the same time, for the phenomena of multi-year inbred parent property degeneration, such as strong plant growth vigor, less bud abortion rate, large pollen amount, and high setting rate, the method finds a novel method for purification and renovation.

The invention discloses a chemical male sterilant composition of cruciferae crops and an application method thereof. The chemical male sterilant composition is prepared by uniformly mixing bensulfuron methyl and isoproturon in a certain ratio. When the chemical male sterilant composition is sprayed in the 'flat head' period of rape, the male killing of the cruciferae rape is thorough, the male abortion rate is 100 percent, the pistil fertility is normal, no adverse effect is caused to growth and development of plants, the outcrossing rate is normal, and the seed hybrid rate is 100 percent. The male sterilant performs 'chemical impurity removal' in rape recessive nucleus male sterile seed production, 'chemical control of micro powder' in cytoplasm male sterile seed production and 'chemical breeding' of common rape varieties. The chemical male sterilant composition of the invention has the advantages of simple method, low production cost, environmental friendliness, easy application and easy popularization.

The invention provides an application of decidua NK cells and cell subsets thereof in preparation of medicines for treating infertility related diseases. Through experimental verification, according to the decidua NK cell and the cell subset thereof, endometrial growth disorder diseases are treated by promoting endometrial thickness increase, enhancing endometrial cell viability, reducing endometrial cell damage, promoting VEGF expression, maintaining endometrial stromal cell dryness and stimulating proliferation; the pregnancy success rate of endometrial injury model mice is increased from 30% to 60-70%; by exerting the immune tolerance effect, reducing the spontaneous abortion rate and improving the T helper lymphocyte level to treat maternal fetal immune tolerance disorder related diseases, a new way is provided for treatment of infertility.

The invention relates to embryo transfer glue and a preparation method thereof and belongs to the technical field of human assisted reproductive technology. The embryo transfer glue is prepared by dissolving recombinant hyaluronic acid, cell clone stimulating factors and human chorionic gonadotropin into a salt culture solution containing antibiotics and an indicator according to the concentration of 0.1-0.8 microgram/ml, 1-20 nanogram/ml and 5-500 unit/ml. The embryo transfer glue has the advantages that endometrium growth is promoted, and effective communication and adhesive ability between an embryo and the endometrium are increased to increase the implantation rate of the embryo and lower abortion rate by adding the recombinant hyaluronic acid, the cell clone stimulating factors and the human chorionic gonadotropin; the embryo transfer glue is a non-animal-origin recombinant bioengineering high-tech product, the possibility of animal-origin pathogens and toxins is eliminated effectively, and the safety of assisted reproduction can be increased greatly.

The invention relates to application of ginsenoside in the pharmacy field, in particular to application of ginsenoside in preparing antiphospholipid syndrome molecular targeting treatment medicine. In a laboratory antiphospholipid syndrome mouse model, the compound can effectively lower the level of anticardiolipin antibodies, anti-beta2-GP1 antibodies and lupus anticoagulants in blood; meanwhile, in a pregnant mouse model, the compound effectively lower aCL activation, shortens partial thromboplastin time and reduces the blood platelet count and the abortion rate; safety study shows that the compound is high in safety, has no influence on the central nervous system, the cardiovascular system and the respiratory system and has no mutagenic action. It is indicated that the compound has high safety and a good inhibiting effect on the antiphospholipid syndrome at the test dose and can be used for treating the antiphospholipid syndrome.

The invention mainly relates to the technical field of cultivation, and discloses a feed special for pregnant ewe. The feed is prepared from corn flour, wheat flour, soybean flour, potato dregs, red alga, dunaliella salina, oilseed rape leaf, cushaw flower, wheat flower, propolis, Chinese yam extracts, apple leaf extracts, hydrolyzed yolk powder, Angel aroma-producing yeast, dry red pepper and rhizoma kaempferiae. The feed is special for the pregnant ewe and used for feeding the pregnant ewe from progestation to the postpartum period to improve the disease resistance, the feed is capable of enhancing the constitution of the ewe and increasing the pregnancy rate before pregnancy and providing rich nutrition during pregnancy, the abortion rate is reduced to 0%, the lambing amount is increased, the average litter yield is 2.71, the morbidity of the pregnant ewe is reduced to 2%, and the economic income of a cultivation farmer is increased by 11.3%.

The invention discloses application of himalayan teasel root total saponins in preparation of miscarriage prevention medicines. By adopting the himalayan teasel root total saponins, expression of estrogen and progestrone receptors in the uterus and ovary of an animal model can be promoted, vaginal bleeding of the animal model is obviously reduced, the live-birth number is obviously improved, the abortion number is reduced, and the abortion rate is lowered; animal pregnancy is protected from multiple levels and multiple factors, and the miscarriage prevention effect is very obvious.

The invention discloses a method for improving the pregnancy conservation efficiency of male seahorses. Due to comprehensive adjustment on factors, such as breeding isolation, illumination intensity, a photoperiod, a bait type and a feeding frequency, in a seahorse pregnancy conservation process, the reproduction efficiency of a male pregnant seahorse is obviously improved. A specific result is that the abortion rate of the male seahorses is reduced from 15-30 percent to 2-5 percent, and the germling incubating rate is also increased from 70-75 percent to 85-90 percent; meanwhile, the body length of young seahorses is increased by 5-9 percent. The method has the advantages of easiness in operation and high controllability, and is suitable for large-scale commercial seahorse breeding.

The invention provides a pregnant sow nursing feed and a preparation method thereof, and relates to the technical field of feed processing. The nursing feed is prepared from the following raw materials in parts by weight: 20-40 parts of corns, 12-16 parts of pumpkin, 20-26 parts of wheat bran, 18-22 parts of soybeans, 4-6 parts of fish meal, 2-4 parts of garlic, 10-12 parts of a traditional Chinese medicine extracting solution, 1-3 parts of choline chloride, 3-4 parts of selenium-enriched yeast, 2-4 parts of a vitamin mixture, 2-3 parts of protease, 0.2-0.6 part of a trace element mixture, 4-6parts of oligosaccharide, 1-2 parts of sodium chloride and 0.8-1.2 parts of dibasic calcium phosphate. The defects in the prior art are overcome, various nutrient substances required by sows during pregnancy can be provided effectively, the immunity of the pregnant sows is enhanced, the abortion rate and the stillbirth rate of the sows are reduced, the survival rate of newborn piglets is high, the body weight of the piglets is increased quickly, and the breeding efficiency is improved.

The invention discloses a feed additive for fetus protection and miscarriage prevention of sows and a preparation method of the feed additive. The feed additive is prepared from, by weight, 40%-50% of cortex eucommiae extract, 20%-30% of milkvetch root extract and 20%-30% of pine tree extract. The pregnant sows are fed by following the traditional Chinese medicine compatibility principle and research of modern pharmacology on traditional Chinese medicine functions, combining the environment where the intensively-bred sows are located and the growth characteristics of the intensively-bred sows and taking agentia prepared from natural Chinese herbs as the feed additive on the basis of understanding and the treating principle of traditional Chinese medicine on the fetus protection and miscarriage prevention mechanism of the sows, therefore, the resistance of pregnant sow organisms can be improved, health can be promoted, the abortion rate is significantly decreased, the weight of neonatal piglets is increased, and the neonatal piglets are not prone to fall ill.

The invention belongs to the field of veterinary drugs, and in particular relates to a composition for preventing abortion of black sows. The composition consists of the following raw materials in parts by weight: 8-12 parts of balsamiferou blumea herb, 15-25 parts of calamus tetradactylus hance, 12-18 parts of herba bidentis bipinnatae, 10-20 parts of glabrous pogestemon herb, 10-20 parts of herba sarcandrae, 2-8 parts of lycoris radiata herb, 12-18 parts of cephalotaxus hainanensis, 8-12 parts of Chinese actinidia roots, 12-18 parts of herba leonuri, 12-18 parts of radix glycyrrhizae, 2-8 parts of munronia unifoliolata, 10-20 parts of vetchleaf sophora root, 4-10 parts of compound amino acid and 4-10 parts of compound vitamin. The composition provided by the invention can take an obviouseffect on preventing abortion of black sows; the phenomenon of abortion of the black sows is not discovered in a clinical test process; an abortion rate is close to zero; a litter size is obviously improved; the survival rate of piglets is up to 98% or above; the effect of preventing the abortion of the black sows is effectively improved; and the composition has important significance for improving the production performance of the black sows, reducing economic losses and promoting the sustainable and healthy development of the black pig breeding industry.