The invention relates to the technical field of pesticide packaging, and particularly discloses a pesticide bottle and a preparation method thereof. The pesticide bottle comprises, by weight, an inner layer, a first bonding layer, a middle layer, a second bonding layer and an outer layer from inside to outside; the inner layer comprises the following raw materials by weight: 20-25 parts of HDPE, 3-5 parts of PE, 2-3 parts of a reinforcing agent, 3-5 parts of an anti-cracking agent, 0.5-1 part of wollastonite, 0.1-0.2 part of a dispersing agent, 1-2 parts of calcium carbonate and 0.3-0.5 part of a titanate coupling agent; the reinforcing agent is prepared from titanium dioxide, alginate fiber and coconut fiber; the preparation method comprises the following steps of: respectively mixing the raw materials of the inner layer, the raw materials of the middle layer, the raw materials of the first bonding layer, the raw materials of the second bonding layer and the raw materials of the outer layer, and uniformly mixing the materials to obtain an inner layer material, a middle layer material, a first bonding layer material, a second bonding layer material and an outer layer material; subjecting the raw materials of all the layers to melt extrusion and then conducting blow molding, cooling shaping and external renovation to obtain the pesticide bottle. The pesticide bottle has the advantage of being high in strength.