The invention relates to a multi-component fresh-keeping formula and a fresh-keeping method of sweet persimmons at room temperature, and belongs to the technical field of persimmon preservation. The multi-component fresh-keeping formula comprises the following raw materials: gibberellin, sodium diacetate, ethanol, a honeysuckle extract, a forsythia extract, a clove extract, a radix stemonae extract, a persimmon leaf extract, a licorice root extract and pure water; 1-methyl cyclopropylene, sec-butylamine, sodium hydroxide and water; chitosan, pectin, potassium permanganate, ethanol, kojic acid, sodium D-isoascorbate, vitamin E, proanthocyanidins and water; and reduced iron powder, granular activated carbon, potassium permanganate, sodium D-isoascorbate, calcium peroxide and sodium polyacrylate. The formula provided by the invention is reasonable in design, the nutrition and taste of the sweet persimmons are maximized, and the fresh-keeping effect is good.