The invention provides recombinant
yarrowia lipolytica with high
grease yield and a construction method of the recombinant
yarrowia lipolytica, and belongs to the field of bioengineering. The
Yarrowia lipolytica strain po1g is used as an original strain to integrally express the
diacylglycerol acyltransferase from the cocoa tree; an acetyl-
coenzyme A carboxylase of
yarrowia lipolytica; a human source stearoyl
coenzyme A deunsaturated
enzyme; a yarrowia lipolytica AMP deaminase; the
aldehyde dehydrogenase of the
escherichia coli obtains a recombinant strain for improving the yield of the
grease of the yarrowia lipolytica. Preferably, the screened recombinant yarrowia lipolytica strain with high
grease yield is named as the yarrowia lipolytica strain GK03, 131 g / L of
fatty acid can be produced after fed-
batch fermentation at a 5L tank level, and efficient synthesis of the
fatty acid in the yarrowia lipolytica is realized. The method provided by the invention can be used for constructing the high-oil-yield
yeast, and has wide application prospects in the fields of
renewable energy sources,
chemical industry,
medicine and health care and the like.