The invention relates to a medical blue laser printing film and a preparation method. The medical blue laser printing film comprises a transparent PET base material, wherein a laser printing coating is arranged on the front surface of the transparent PET base material, and a blue antistatic layer is arranged on the back surface of the PET base material; the preparation method comprises the following steps: step 1, preparing the coating material of the laser printing coating; step 2, coating to form the laser printing coating: performing slope flow extrusion coating, heating with steam, drying, and enabling the drying temperature to reach 100 DEG C or above and the dry basis thickness of the coating to reach 10 micrometers; step 3, preparing the coating material of the blue antistatic layer; step 4, coating to form the blue antistatic layer: performing slope flow extrusion coating, heating with steam, drying, and enabling the drying temperature to reach 100 DEG C or above and the dry basis thickness of the coating to reach 2-2.5 micrometers. The medical blue laser printing film prepared by adopting the preparation method is suitable for high resolution laser printing, anti-adhering, low in friction coefficient, static resistant, high in adhesion capability, good in glossiness, blue and transparent in appearance, acid-base resistant, high in wear resistance, environment-friendly, simple and convenient in manufacturing method, and suitable for batch production.