The invention discloses an industrial low-temperature whitening washing powder, which is prepared from the following components according to a certain proportion: powdery pentahydrate sodium metasilicate, sodium carboxymethylcellulose, sodium nitrilotriacetic acid, lipase, amylase, protease, fluorescent agent, fatty alcohol-polyoxyethylene ether, amphoteric imidazoline, triclosan disinfectant, sodium perborate monohydrate, non-phosphorous additive and fatty acid potassium. The preparation method of the brightened goods washing detergent comprises the steps of firstly putting the weighted powdery pentahydrate sodium metasilicate, sodium perborate monohydrate, fatty acid potassium and sodium carboxymethylcellulose into a stirring kettle through a filter screen for 10 minutes; while stirring, simultaneously adding the non-phosphorous additive to the stirring kettle for three times, and continuously stirring for 3 minutes; pre-stirring the triclosan disinfectant, the amphoteric imidazoline and the fatty alcohol-polyoxyethylene ether for 4 minutes, and adding thetriclosan disinfectant, the amphoteric imidazoline and the fatty alcohol-polyoxyethylene ether to the stirring kettle for stirring for 4 minutes; adding the sodium carboxymethylcellulose to the stirring kettle through the filter screen for stirring for 5 minutes; uniformly scattering the lipase, the amylase and the protease into the stirring kettle for stirring for 3 minutes; and finally putting the fluorescent agent into the stirring kettle for stirring for 4 minutes.