178 results about "EM - Electron microscopy" patented technology

The present invention relates to the technical field of diabetes, and in particular to a controlled-release diabetic wound healing promoting treatment method, comprising the following treatment experimental steps: step S1: preparation of Hst1@PNPs; step S2: Coomassie brilliant blue staining; step S3: scanning and transmission electron microscopy detection; in the present invention, a slow-release Hst1 treatment method is constructed by encapsulating Hst1 into platelet membrane vesicles derived from autologous blood. On the one hand, the PNPs encapsulate the linear Hst1 peptide structure to prevent it from being rapidly degraded by high proteases in diabetic wound exudate, thereby improving bioavailability. On the other hand, the PNPs can adsorb excess LPS in the wound exudate, and cooperate with Hst1 to achieve anti-inflammatory and anti-oxidative stress effects. Subsequent cell and animal experiments further confirmed that Hst1@PNPs have the effects of anti-inflammatory, scavenging ROS, promoting re-epithelialization, collagen deposition and angiogenesis, thereby promoting diabetic wound healing.

The invention discloses a method for evaluating the growth performance of mangrove forest seedlings in an estuary high-salinity environment, and the method comprises the steps: constructing an analysis framework in which a dynamic salinity stress system and a multi-dimensional monitoring technology are coupled, and achieving the precise evaluation of the environmental adaptability of the mangrove forest seedlings; the method comprises the following steps: firstly, carrying out sterile germination and matrix domestication on a target estuary provenance, then establishing a three-dimensional stress model containing basic salinity maintenance, sudden salinity impact and tidal cycle fluctuation, and strictly controlling the sudden salinity impact amplitude within 20% of the historical extreme value of the estuary; synchronously acquiring canopy volume and root architecture parameters of the seedlings by adopting a three-dimensional laser scanning technology, and constructing a morphological database in combination with leaf apparent characteristic records; subcellular structure indexes such as cell wall thickening, chloroplast envelope integrity and mitochondrial ridge density are observed and analyzed through a transmission electron microscope, the molecular mechanism of osmotic regulation and energy metabolism is disclosed, and the concentration of suspended solids in the water body, the oxidation-reduction potential of sediments and the functional diversity of microorganisms are synchronously monitored.

The invention discloses a preparation method of a transmission electron microscope sample of a sintered neodymium-iron-boron block material, which comprises the following steps of block sample cutting, mechanical grinding, sample punching, ring sticking, pre-thinning and final thinning, so that the transmission electron microscope sample to be observed is obtained. In addition, the method is simple, feasible, economical and practical, and the sample preparation success rate can be remarkably increased.

The invention discloses a transmission electron microscope sample preparation method for a lunar soil space weathered surface and application, and the method comprises the following steps: pasting target lunar soil particles on a carbon conductive adhesive tape, and enabling the target flat surfaces of the target lunar soil particles to be placed back to the carbon conductive adhesive tape; plating a layer of carbon film on the surface of the target lunar soil particle to obtain a scanning electron microscope sample of the target lunar soil particle; screening out a plurality of target research areas on the carbon film corresponding to the target flat surface; uniformly plating a layer of gold film on the carbon film corresponding to the target lunar soil particle scanning electron microscope sample; deposition operation of a research area protection layer is carried out on the gold film corresponding to the target research area through an electron beam and an ion beam in sequence; and performing slicing operation perpendicular to the target flat surface on the target lunar soil particle scanning electron microscope sample to obtain the target lunar soil particle transmission electron microscope sample. According to the method, lunar soil space weathering characteristics can be truly reserved, and an accurate and reliable sample is provided for lunar soil space weathering research.

The invention discloses preparation of an aqueous zinc ion battery positive electrode material and a detection method of charge reaction of the aqueous zinc ion battery positive electrode material in a metal ion battery, and belongs to the technical field of batteries. The invention provides the preparation method of the vanadium-based positive electrode material, which is simple in process and low in cost. The preparation method of the positive electrode material comprises the following steps: grinding vanadium pentoxide powder; performing ultrasonic treatment with deionized water, adding inorganic salt, sealing, performing water bath heating reaction, and performing centrifugal washing; and then drying or freeze-drying to finally obtain the batched and high-performance positive electrode material. In 8000 times of charging and discharging, the positive electrode material provided by the invention has excellent cycling stability, and the capacity retention rate is as high as 93%. In addition, an in-situ alternating-current impedance technology is used for rapidly detecting the charge transfer process in the battery, and complex and expensive in-situ X-ray diffraction and transmission electron microscope technologies are avoided. The vanadium pentoxide activation process is accurately controlled through the water bath, the process is simple, the cost is low, and the method has good batch production potential and application prospects.

The invention discloses an ion thinning instrument control method based on a self-adaptive gradient energy reduction algorithm, and belongs to the technical field of transmission electron microscope (TEM) sample preparation. The problem that a traditional ion thinning technology has many limitations is solved, dynamic optimization of parameters such as ion beam energy, beam current density and sample temperature is achieved through the self-adaptive gradient energy reduction algorithm, and the sample preparation efficiency is remarkably improved; by combining multi-modal data acquisition and dynamic gradient optimization, the thinning process can be accurately controlled, and the sample quality is improved; sample damage is effectively avoided by combining a damage constraint mechanism and self-adaptive step length control, and the stability of the preparation process is improved; the method is suitable for various materials through a pre-stored optimization path library, and has wide applicability; therefore, the energy of the ion beam is optimized, the incident angle and the beam density are dynamically adjusted, and the thinning instrument is controlled.

Methods for preparing imaging samples by applying a liquid or semiliquid substance to a substrate surface in order to mitigate the deleterious effects of vacuum and other conditions used for sample preparation, thereby preserving the structure of at least a portion of deposited samples. These methods are useful for MS-based electron microscopy (EM) sample preparation at room temperature, but are also applicable to other imaging methods. These methods also enable transmission electron microscopy (TEM) structural confirmation of ions passing through a mass spectrometer. Additionally, only entry-level negative staining techniques for TEM are required, making this technique much more widely available than cryogenic electron microscopy (cryo-EM).

The invention discloses an application of a cucumber CsAPRR2 gene in regulating and controlling the color of pericarp. The invention relates to the field of plant genetic engineering, and provides application of inhibiting expression and / or activity of CsAPRR2 protein in creation of yellow peel cucumber. CsAPRR2 is knocked out through a CRISPR-Cas9 mediated gene editing technology, all knockout mutation lines show the pericarp yellowing character, a transmission electron microscope (TEM) shows that the chloroplast structure of the CsAPRR2 knockout line is damaged, and it is proved that the CsAPRR2 gene can regulate and control the color of the pericarp of the cucumber. The invention provides a molecular tool and a gene resource for researching a cucumber peel yellowing regulation mechanism and cultivating a new variety.

The present invention discloses the application of the NtIPMD gene in regulating the chlorophyll content of tobacco, and belongs to the field of plant genetic engineering technology. Based on the existing progress in tobacco genetic engineering and the observation and identification of related plant phenotypes, the present invention conducts in-depth research on the NtIPMD gene and finds that it is related to the development of tobacco chloroplasts. The nucleotide sequence of the NtIPMD gene is shown in SEQ ID NO.1. After silencing the gene, the chlorophyll content of the leaves decreases, the growth of the plants slows down, and transmission electron microscopy observation reveals abnormal chloroplast development. The present invention finds that the gene encoding tobacco isopropylmalate dehydrogenase, NtIPMD, plays a key role in regulating the chlorophyll content of tobacco leaves, laying the foundation for clarifying the molecular regulatory mechanism of chlorophyll biosynthesis in tobacco leaves and providing a new precise regulatory gene for regulating the chlorophyll content of tobacco leaves.

The invention provides selenium nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of selenium nanoparticles. Acetone ultrasonic cleaning and chemical polishing pretreatment are performed on a pure titanium substrate to obtain a clean surface, an anodic oxidation method is adopted to prepare a # imgabs0 # nanotube array carrier, and the nano-particles are prepared into selenium nano-particles. The method comprises the following steps: accurately depositing selenium nanoparticles on a carrier by using an electrochemical deposition method, realizing load control by combining an electrochemical kinetics principle, realizing uniform dispersion of the particles by matching an ultrasonic dispersion technology with a particle swarm optimization algorithm, and carrying out centrifugal separation and vacuum drying to obtain a spherical selenium nanoparticle product. And a multi-dimensional quality control detection system of a transmission electron microscope, an X-ray photoelectron spectroscopy and dynamic light scattering is established, so that the technical problems that the particle size distribution is non-uniform and the deposition load is difficult to accurately control in the preparation process of the selenium nanoparticles are solved.

The invention discloses a toughening forming method of a high-performance PEI plate for a high-heat-resistance structural part, and particularly relates to the technical field of high-performance composite material forming. Pre-embedding a PEI toughening layer between thermosetting prepreg layers, and controlling the temperature, the pressure, the pressure maintaining time and the heating rate in the hot pressing process; carrying out infrared spectrum or solid nuclear magnetic resonance testing on the interface area sample subjected to hot press molding, and calculating the chain segment residual rate Rp; determining the interface penetration depth Di through a transmission electron microscope in combination with energy spectrum analysis; inputting the Rp and the Di into an interface coupling model, and outputting an interface combination quality factor Q by the model in a form of combining weighted linearity and logarithmic functions; the interface bonding grade is judged according to the Q value, and when the Q value is lower than a threshold value, hot-pressing process parameters are backtracked for optimization; according to the method, quantitative evaluation of the interface performance and process closed-loop optimization are realized, and the interface bonding strength and service reliability of the composite material can be effectively improved.

This invention relates to a method for locating characteristic defects in cadmium zinc telluride (CZT) samples and a method for preparing CZT samples. The location method includes the following steps: determining the positions of internal feature points on the sample under an infrared transmission microscope; establishing marking features on the sample surface using FIB-SEM; and determining the relative position of the internal feature points projected onto the sample surface by establishing a geometric relationship between the virtual position of the internal feature points projected onto the sample surface and the marking features. The preparation method uses these relative positions to prepare CZT samples. The location method of this invention can simply and effectively establish the positional relationship between internal feature points and surface features, accurately locating internal defect features in CZT samples. This allows for convenient and rapid determination of the specific location for preparing transmission electron microscopy (TEM) samples, thus improving the quality of TEM samples.

This invention provides a rapid preparation method for steel samples for transmission electron microscopy (TEM), relating to the field of physical analysis of steel materials. It is applied to the preparation of various steel samples, including heat-sensitive steels, using a composite binder of cellulose acetate, acetone, silica, and alumina. The binder is used to adhere one side of a wire-cut steel sample sheet to the sheet fixing surface of a grinding aid. A grinding aid area, parallel to the bottom surface of the sheet fixing surface, is provided on the grinding aid device. The grinding aid area is pressed, and sandpaper with progressively increasing grit is used to grind one side until a first preset thickness is reached. Then, the other side is switched, and sandpaper with progressively increasing grit is used to grind the second side until a second preset thickness is reached. The ground sheet is removed, thinned, punched, and electropolished to obtain the target steel sample. This invention can rapidly prepare steel sample sheets with a thickness of 30-50 µm, and the method is simple and has a high success rate.

A method for analyzing a sample using an electron microscope includes: generating ions, molecules, or other particles, such as droplets, from a target sample; introducing the ions, molecules, or other particles into a vacuum cavity of a transmission electron microscope (TEM) in a direction intersecting the path of a pulsed electron beam; recording an electron diffraction pattern associated with the ions through which the pulse passes at each pulse of the electron beam; and calculating the three-dimensional (3D) structure of the ion species based on the recorded electron diffraction pattern.

The present invention relates to the field of image recognition, and proposes a transmission electron microscopy identification method and system for the separation process of exosomes in gastric cancer tumor tissues. By using a clustering algorithm to divide the suspected structures in the target lens image, a primary evaluation of the exosome manifestation degree at the edge is carried out based on the edge differentiation degree and the degree of darkness outside the edge of the suspected structure. Then, a secondary evaluation is combined with the background staining change state of the suspected structure, avoiding the influence that the contour of the background impurities is relatively deep in the prior art, resulting in low differentiation between impurities and exosomes, and improving the accuracy of identification. Furthermore, the morphological matching degree of exosomes is calculated according to the internal depression characteristics of the suspected structure, avoiding the problem that the accuracy of the identification result is affected by the difference in the facing situation of different structures in the lens image and the observation perspective, and realizing the auxiliary analysis of the depression characteristics of exosomes under different facing situations of the perspective. The present invention improves the accuracy of transmission electron microscopy identification in the separation process of exosomes in gastric cancer tumor tissues.

The invention belongs to the technical field of reliability analysis of devices, and particularly relates to a quantitative analysis method for migration characteristics of an indium component in an epitaxial wafer. Comprising the following steps: providing epitaxial wafers with consistent initial characteristics, and carrying out uniform cleavage; taking one small block as a reference sample, and performing external disturbance treatment on other small blocks; the method comprises the following steps: respectively carrying out non-destructive structure characterization treatment on a reference sample and a sample subjected to external disturbance treatment, including high-resolution X-ray diffraction test, photoluminescence and cathode fluorescence combined test and time-resolved photoluminescence spectrum measurement; comprising high-resolution transmission electron microscope and EDS energy spectrum combined imaging and secondary ion mass spectrum; finally, FP lasers are prepared respectively, and electrical characterization processing is carried out. And quantitatively analyzing the influence of indium component migration on the electrical properties of the FP laser according to the result. The method has the advantages that the migration of indium atoms is analyzed according to a method for changing the microstructure in the quantum well and changing the dynamic behavior of a carrier.

The present application provides a kind of negative electrode for reducing the initial resistance of non-aqueous electrolyte secondary battery and achieving the improvement of durability.The negative electrode for non-aqueous electrolyte secondary battery disclosed herein has a negative electrode current collector and a negative electrode active material layer formed on the negative electrode current collector, and the negative electrode active material layer at least contains a first negative electrode active material and a second negative electrode active material.The first negative electrode active material is composed of agglomerates of flaky graphite coated with low-crystallinity carbon on at least part of the surface, and the interlayer distance of graphite of the low-crystallinity carbon based on the electron diffraction image obtained by transmission electron microscopy is 0.3375 nm.The second negative electrode active material is natural graphite or artificial graphite with an interlayer distance of graphite based on the electron diffraction image obtained by transmission electron microscopy of 0.3375 nm.Here, the mass ratio of the first negative electrode active material to the second negative electrode active material is 50:50 to 90:10.

A Pt@Ag platinum-silver nanoalloy and its preparation method are disclosed, specifically comprising: preparing small-diameter platinum nanoseeds using sodium borohydride as a reducing agent; preparing larger-diameter platinum nanoparticles using ascorbic acid as a reducing agent; and constructing a core-shell platinum-silver nanoalloy (Pt@Ag) using the larger-diameter platinum nanoparticles as templates. The internal fine structure, morphology, and optical properties of the prepared platinum-silver nanoalloy are characterized using transmission electron microscopy and ultraviolet absorption spectroscopy. This invention modifies and improves upon previous methods for preparing gold-silver core-shell nanoalloys, exploring a novel preparation technique for platinum-silver nanoalloys. The platinum-silver nanoalloy prepared by this invention is based on nanochemical preparation technology, and its synthesis method is simple, low-cost, and exhibits good biocompatibility, showing promise as a potent nano-radiosensitizing material in the radiotherapy of malignant tumors.

A supported catalyst particles include oxide carrier particles and noble metal particles supported on the oxide carrier particles, wherein the mass of the noble metal particles is less than or equal to 5 mass % based on the mass of the oxide carrier particles, and the average particle size of the noble metal particles measured by transmission electron microscopy is 1.0-2.0 nm, with the standard deviation σ less than or equal to 0.8 nm.

The invention relates to a transmission electron microscope high-resolution in-situ fluid turbulence heating chip, which has the structure that an upper chip and a lower chip are combined through a metal bonding layer to form a self-sealed ultrathin chamber; the upper chip has two sample injection ports and an upper central window; the lower chip is provided with a fluid inlet, a fluid outlet, a fluid flow channel, a lower central window, a micro-turbulence column array, a heating layer and an insulating layer; the heating layer has four contact electrodes and a spiral ring heating wire; the fluid inlet and the fluid outlet are symmetrically arranged about the central window, with the lower central window being the center and in the central area of ​​the spiral ring heating wire; the lower central window is located at the center of the heating layer; the micro-turbulence column array is arranged on the lower central window, and the chip has the advantages of rapid temperature rise and fall, high resolution, precise temperature control, controllable fluid flow direction and low sample drift rate.

The invention discloses a large-view-field and high-temporal-spatial-resolution transmission electron microscope in-situ imaging method for a microcosmic electrochemical process, which comprises the following steps: step 1, loading an electrode material particle sample to be detected on a carrying table, inserting the sample into a transmission electron microscope vacuum lens cone along with a sample rod, identifying a target sample under low magnification, and carrying out basic adjustment on an electron microscope; 2, adjusting magnification times to ensure that an observation area is completely incorporated into an imaging view field, selecting an imaging mode, eliminating astigmatism, and adjusting the focal length of an objective lens until a sample particle image interface is clear; and step 3, selecting a CCD camera, setting an acquisition frame rate, and performing ultrafast acquisition of images or videos. The transmission electron microscope in-situ imaging technology giving consideration to both the large view field and the high space-time resolution has practical application value in research of the electrochemical reaction kinetics process of the granular material.

The present disclosure provides a process for synthesizing a cross-linked covalent organic framework (COF) in which polypyrrole chains connect the two-dimensional (2D) COF layers through covalent bonds via post-synthetic modifications to form quasi-three-dimensional (quasi-3D) COF with improved electronic conductivity over its 2D COFs without the loss of 5 crystallinity. Interlayer cross-linking improves the layer alignment leading to a decrease in the band gap and an increase in inter-layer charge mobility or electronic conductivity of the 3D COFs. The structural robustness of COFs is crucial for it to qualify for several practical applications. The 3D COFs obtained by the presently claimed process display remarkable improvement of crystallinity evidenced through SAED (Selected area electron diffraction) 0 pattern and lattice fringes in high-resolution transmission electron microscopy (HRTEM) which results in improved electronic conductivity.

The invention discloses a construction method of a COL4A5-K229X point mutation X-linked Alport syndrome mouse model. The construction method comprises the following steps: aiming at c.685Agt of a No.12 exon of a mouse COL4A5 gene; carrying out T point mutation, and designing and preparing Cas9 mRNA, gRNA and a donor vector; the components are mixed and then microinjected into fertilized eggs of a C57BL / 6J mouse to obtain an F0-generation mouse; identifying the genotype through PCR (Polymerase Chain Reaction) amplification and Sanger sequencing, and screening positive mice; mating the positive F0-generation mice with the wild-type mice, and breeding F1-generation and subsequent generations; the phenotype of the model is further verified through qPCR, biochemical analysis, light microscopic examination, transmission electron microscope and immunofluorescence. The model constructed by the invention shows hematuria, proteinuria, azemia, podocyte loss and irregular thickening and layering of glomerular basement membrane, is consistent with phenotypes of human XLAS patients, and provides an animal model tool for analyzing pathogenesis and developing treatment strategies.

In one aspect, a flow cell (100) for liquid-phase transmission electron microscopy comprising an imaging portion (140) and a surrounding portion (150) in fluid communication with the imaging portion is provided. A height (153) of the channel (130) in the surrounding portion is configured to be greater than a height (143) of the channel in the imaging portion; and a length (164, 174) of the imaging portion is selected to achieve mass transport of the one or more fluids preponderantly by diffusion in the imaging portion. In a further aspect, a method of manufacturing the flow cell is provided.

The present invention comprises an active phase comprising manganese (Mn), at least one first element of group VIII chosen from iron (Fe), cobalt (Co), and nickel (Ni), said catalyst comprising a porous support comprising at least one refractory oxide chosen from titanium dioxide, silica, alumina, silica-alumina, zirconia, ceria, used pure or in a mixture with each other intended particularly for the reduction of nitrogen oxides (NOx). Said catalyst comprises between 0.1 and 15% by weight of element manganese and between 0.1% and 20% by weight of element of group VIII relative to the total weight of the catalyst such that the molar ratio between manganese and the element of group VIII is between 0.1 and 30 mol / mol. The active phase is characterized in the form of nanoparticles of size between 1 nm and 30 nm, measured by transmission electron microscopy.

The present application relates to the field of transmission electron microscopy characterization of two-dimensional material structure, and particularly to a method for efficiently obtaining cross-section characteristic crystal orientation of two-dimensional material, which is suitable for two-dimensional van der Waals layered material and two-dimensional non-layered material. A focused ion beam microscope (FIB) is used to sample in the direction perpendicular to (or parallel to) the long straight edge of the two-dimensional material edge, and the two-dimensional material sampling direction is parallel to the half-moon-shaped carrier net string length direction for welding, so as to prepare a cross-section sample capable of reflecting atomic layer stacking sequence; the half-moon-shaped carrier net string length direction is placed perpendicular to the TEM sample rod axial direction, and then small-angle tilting is performed to quickly realize sample crystallographic orientation. The present application lays a foundation for efficiently analyzing crystallographic information such as crystal structure, stacking defects and surface reconstruction of the material by observing the cross-section sample of the two-dimensional material.

The invention provides a coenzyme Q10 nano-liposome capable of being endocytosed by brain neurons as well as a preparation method and application of the coenzyme Q10 nano-liposome, and belongs to the technical field of biological medicines. According to the coenzyme Q10 nano-liposome and the preparation method thereof, polycarbonate filter membranes of 220 nm, 100 nm and 50 nm are adopted to sequentially extrude a solution, the coenzyme Q10 nano-liposome which has the particle size range of 30-50 nm, is not prone to melt collapse and can be endocytosed by neurons is prepared, and the preparation method is simple and efficient. According to the invention, nano-liposomes simultaneously wrapping 5nm gold and coenzyme Q10 are further prepared, distribution of the nano-liposomes can be traced through a transmission electron microscope, and whether the drug coenzyme Q10 enters extracellular gaps of brain cells and whether the drug coenzyme Q10 is endocytosed into neurons or not is verified. The coenzyme Q10 nano-liposome disclosed by the invention can penetrate through a blood brain barrier, enter extracellular gaps and are endocytosed by neurons to eliminate formaldehyde accumulated in the brain, so that a foundation is provided for research and development of drugs for brain diseases such as Alzheimer's disease.

This invention discloses a method for preparing amorphous Cu-MOFs and their applications, relating to the field of metal-organic framework materials technology. The key technical points are: CuCl2 is used as the copper source and 4,4′-bipyridine as the ligand, and Cu-MOFs are synthesized via a solvothermal method. Transmission electron microscopy shows that the obtained material exhibits a typical micron-scale sheet-like morphology. After etching with ammonia, the sample morphology transforms into rough-surfaced nanospheres with a significantly increased pore size, indicating a local rearrangement of its framework structure. X-ray diffraction analysis shows that the original Cu-MOF has high crystallinity, while the diffraction peaks of the etched aCu-MOF essentially disappear, exhibiting amorphous characteristics. As a signal conversion element in a colorimetric sensor, aCu-MOF can achieve reliable detection of ampicillin under neutral conditions. Using thiram as a cofactor, a highly selective thiram colorimetric sensing system can be constructed.

According to the present disclosure, a novel system or method for electron beam imaging or electron beam spectroscopy is provided, the system includes a transmission electron microscope (TEM) including an electron source configured to provide an electron beam, at least one laser-based device configured to provide at least one laser beam, the laser beam being configured to shift a predetermined portion of the electron beam to an energy spectrum different from the original energy spectrum provided by the electron source, and an electron energy filter configured to pass the electron beam having the original energy spectrum but not pass the electron beam having the energy spectrum different from the original energy spectrum.

The invention belongs to the field of biological medicine, and particularly relates to an application of rose natural extracellular vesicles in preparation of cardiovascular protection products, the rose extracellular vesicles are extracted through a classical ultracentrifugation method, biological characteristics of the rose extracellular vesicles are identified and confirmed through a transmission electron microscope (a tea tray type nano vesicle structure) and a nano-particle tracking analyzer (80-150 nm, 1.8 E + 11 Particle / mL) in a combined mode, and the application of the rose extracellular vesicles in preparation of cardiovascular protection products is achieved. After research, it is found that when the rose extracellular vesicles are applied to cardiovascular products, the rose extracellular vesicles have the effects of (a) promoting endothelial cell proliferation; (b) inhibiting endothelial cell apoptosis; and (c) a function of enhancing oxidation resistance. The invention verifies the application of the rose natural extracellular vesicles, and the experimental research finds that the rose extracellular vesicles as a natural active ingredient have a remarkable effect in synchronous triple regulation (proliferation promotion-apoptosis resistance-oxidation resistance) of human umbilical vein endothelial cells (HUVEC), and can be used for preparing the rose natural extracellular vesicles. And a new way is provided for developing non-engineering cardiovascular protection products with high safety performance.