39 results about "Food borne disease" patented technology

The invention relates to protein feed rich in small peptides and multi-enzyme strains and a preparation method thereof. The protein feed is prepared by fermenting soybean meal, cottonseed meal, rapeseed meal, bone meal, wheat bran, common salt, light calcium carbonate, water and strains in a fermentation bin. The preparation method of the protein feed comprises the following steps: mixing the soybean meal, the cottonseed meal, the rapeseed meal, the bone meal, the wheat bran, the common salt and the light calcium carbonate in percentage by weight; adding 20-30 percent of water; sending the mixture to an air steamer; adding high-pressure steam to the air steamer so as to increase the air pressure to 1-2 MPa and the temperature to 140-200 DEG C; keeping the pressure for 4-6 minutes; momentarily releasing the mixture in the air steamer; sending the mixture to the fermentation bin; inoculating 3-5 percent of strains when the temperature of the mixture falls to 30-37 DEG C; fermenting for 72 hours at the temperature of 32-37 DEG C; discharging from the bin; and bagging to obtain a finished product. The product has strong disease-resistant function and low cost, increases the productionof animal by-products by more than 20 percent, is nontoxic, harmless and pollution-free and can inhibit food-borne diseases represented in cancers and subhealth phenomena from spreading.

Compositions comprising chelating agents, metal ion salts, gelling agents or a buffer, antimicrobials, antibiofilm agents and a pH adjuster or a buffer for the prevention and treatment of wound infections and food-borne diseases involving bacterial biofilms are disclosed. The anti-infective properties of a composition include reduction or killing of anaerobic / aerobic / facultative gram-negative and gram-positive wound infection associated bacteria occurring in polymicrobial biofilms. The composition may be in the form of lotion, cream, ointment, dressing, bandage, rinse, soak, gel, spray, or other suitable forms, including certain devices. Additionally, the invention offers an efficient method of delivering the formulated composition containing one or two chelating agents or chelating agents alone or in combination with a metal ion salt using either a nanoparticle or other efficient delivery systems.

The invention belongs to the technical field of microorganism, and in particular relates to high cohesion pediococcus pentosaceus screened from fermented bean products and use thereof in purifying of vibrio parahaemolyticus in water body. The pediococcus pentosaceus F28-8 is preserved in China General Microbiological Culture Collection Center in 2014 November 13, and the preservation number is CGMCC No.9956. The pediococcus pentosaceus has high cohesion activity, can be used for bacteria-reducing and purifying treatment of vibrio parahaemolyticus in water body to avoid use of a chemical antibacterial agent and antibiotics for water bacteria-reducing and purifying treatment, provides new means for breeding and biological control of vibrio parahaemolyticus in temporary rearing water, and is conducive to the prevention of the spread of aquatic animal disease and food borne diseases caused by the vibrio parahaemolyticus.

The invention discloses a pathogenic vibrio phage VmYZU10474 and application thereof. The phage is identified to have unique genome structure characteristics, belongs to a novel phage, and has been preserved in China Center for Type Culture Collection on August 12, 2020 with the preservation number of CCTCC NO: M 2020417. The phage capable of efficiently cracking vibrio is obtained, can inhibit pathogenic vibrio in various matrixes, such as mimicus vibrio, vibrio parahaemolyticus, vibrio alginolyticus and vibrio fluvialis, and can be applied to preparation of bacteriostatic agents for controlling pathogenic vibrio pollution in aquatic products and environments thereof. The prepared bacteriostatic agents can effectively control growth of pathogenic vibrio in culture media, fish juice and aquatic product samples, and meanwhile can effectively inhibit pathogenic vibrio pellicle formation on solids such as equipment and vessels; and in addition, the pathogenic vibrio phage VmYZU10474 is simple to prepare and convenient to use, and the risk that pathogenic vibrio is spread and causes food-borne diseases can be reduced.

Compositions comprising chelating agents, metal ion salts, gelling agents or a buffer, antimicrobials, antibiofilm agents and a pH adjuster or a buffer for the prevention and treatment of wound infections and food-borne diseases involving bacterial biofilms are disclosed. The anti-infective properties of a composition include reduction or killing of anaerobic / aerobic / facultative gram-negative and gram-positive wound infection associated bacteria occurring in polymicrobial biofilms. The composition may be in the form of lotion, cream, ointment, dressing, bandage, rinse, soak, gel, spray, or other suitable forms, including certain devices. Additionally, the invention offers an efficient method of delivering the formulated composition containing one or two chelating agents or chelating agents alone or in combination with a metal ion salt using either a nanoparticle or other efficient delivery systems.

The invention discloses a method for identifying life or death of cells of food-borne pathogenic bacteria. The identification method comprises the following steps of: adding cell bacteria suspension of the food-borne pathogenic bacteria into EMA with final concentration of 5 to 50 microgram / milliliter and standing the mixture in dark for 3 to 10 minutes; exposing the mixture for 5 to 15 minutes by using a halogen lamp under the condition of ice bath; extracting the DNA of the bacteria solution; performing PCR and electrophoretic analysis; and proving that the cells of the food-borne pathogenic bacteria are living cells when a specific amplification fragment occurs or dead cells when no specific amplification fragment occurs. By the identification method, the life or death of the food-borne pathogenic bacteria, particularly the vibrio parahaemolyticus, can be rapidly detected, false positive result can be prevented in the detection of an actual sample, reliable basis can be provided for the prevention or control of the food-borne diseases and a scientific method and a theoretical basis can be provided for the sanitary inspection of food and the inspection work of the import and export commodities.

The invention relates to a multiplex amplification internal standard PCR (polymerase chain reaction) kit capable of simultaneously detecting Vibrio parahaemolyticus, Escherichia coli O157:H7, Salmonella and Shigella. The kit comprises a PCR reaction premix solution and a reference substance. The detection method comprises the following steps: acquiring a sample, carrying out amplification culture, extracting DNA (deoxyribonucleic acid), preparing a PCR reaction tube, putting the PCR reaction tube on a PCR instrument, carrying out PCR amplification, and carrying out detection result judgment on the PCR amplification product. The kit can simultaneously and quickly detect the four bacteria by one reaction, and can avoid the misjudgment caused by the false negative result due to the inhibiting factor for the DNA polymerase in the sample treatment process. Compared with the existing detection methods, the method provided by the invention is convenient to use, has the advantages of high reliability, short detection period, high sensitivity, high specificity, low cost and simple operation steps, is suitable for high-flux operation and standard operation, and is beneficial to enhancing the food safety detection level and preventing and controlling the foodborne diseases.

The invention discloses a biological sensing method for quantitatively analyzing cholera toxin, wherein a phospholipid bilayer membrane covered Au nanoparticle probe modified by Raman dye is prepared; functional phospholipid, namely phospholipid with a modified sulfydryl at the head end thereof, is selected; and the functional phospholipid can be covalently bound to the surface of Au nanoparticles so that the stability of the Au nanoparticle probe is improved. Cholera toxin subunit B (CTB) is specifically bound with a plurality of acceptor GM1 molecules, thus resulting in the agglomeration of Au nanoparticles covered with GM1 molecules; as a result, surface plasmas are coupled, thereby realizing high-sensitivity SERS (Surface Enhanced Raman Scattering) detection. Simultaneously, the method is capable of causing the change of the light absorption property and also capable of performing detection by a colorimetric method. The method is simple and quick in operation steps, free of applying any mark to the substrate, low in cost, high in sensitivity and good in specificity; the method is hopeful to become a useful technical way for detecting or identifying the cholera toxin; and the method plays an important role in monitoring food security, and controlling and preventing food-borne diseases.

Campylobacter jejuni is a leading cause of bacterial food-borne diseases in humans, ranging from acute diarrheal disease to neurological disorders. An isolated or purified antibody or fragment thereof specific to C. jejuni is described. The antibody or fragment thereof binds to a flagellar protein and reduces motility of C. jejuni. The antibody or fragment thereof is derived from a heavy chain IgG variable domain fragment (VHH) of a camelid animal immunized with C. jejuni flagellar protein. A multivalent form, as well as a phage format, of the antibody or fragment thereof is described. Methods of reducing presence of C. jejuni in an animal or an animal environment, methods and formulations for treating C. jejuni infection, and method of detecting C. jejuni are also described.

The invention discloses a highly-sensitive and rapid method for detecting food-borne pathogenic bacteria based on a signal cascade double-amplification system. The method mainly comprises the following steps: (1) coupling a polyclonal / monoclonal antibody of pathogenic bacteria to the surface of a magnetic bead to obtain an immunomagnetic bead; (2) mixing the immunomagnetic bead with a sample containing food-borne pathogenic bacteria, and specifically capturing the pathogenic bacteria through antigen-antibody reaction; (3) initiating a hybridization chain reaction by using the pathogenic bacteria aptamer modified by the initiation sequence and a DNA hairpin structure probe to obtain an HCR product (double-stranded DNA with a notch); and (4) adding Raman signal molecules and a Raman substrate into the HCR product, and carrying out corresponding SERS determination to realize cascade amplification and output of signals. The method has great significance in guaranteeing food safety and preventing food-borne diseases, and has great application potential for other biological analysis.

The invention belongs to the technical field of bacteria detection, and specifically discloses a method for accurately determining bacteria in special condition on basis of microfluidic visualizationtechnology as well as selecting and enriching the bacteria in the special condition. The method for accurately determining the bacteria in the special condition on basis of the microfluidic visualization technology as well as selecting and enriching the bacteria in the special condition comprises the following steps: preparing bacterium fluid into microdroplets by adopting a microfluidic technology; and then, culturing the prepared microdroplets, taking photos of the cultured microdroplets, and carrying out counting by performing manual counting or in virtue of a computer technology and the like so as to obtain the number of the bacteria in the special condition. Moreover, the method also enables selection and enrichment of the bacteria in the special condition. Compared with existing counting mode adopting plate counting, or only counting living bacteria with complete membranes, the method for accurately determining the bacteria in the special condition on basis of the microfluidic visualization technology as well as selecting and enriching the bacteria in the special condition has greatly improved accuracy, and breaks through the existing counting mode of the bacteria in the special condition, so that food hygiene status can be timely and effectively judged; and thus, the method is an effective means for eliminating foodborne diseases caused by recessive remains of foodbornepathogens. In addition, the method is capable of performing selection and enrichment on the bacteria in the special condition, so that result error caused by cell heterogeneity in research process canbe reduced; and thus, the method is of relatively great value to studies on mechanisms of cell changes and bacteria growth activities.

The invention discloses a food-borne disease pathogenic factor prediction method based on a BP neural network, and the method comprises the following steps: S1, collecting data, collecting and arranging food-borne disease accident cases, building a food-borne disease sample analysis database, and recording the feature items contained in each sample; and S2, determining a training set and a test set, and performing attribute selection and neuron definition. The method has the beneficial effects that the deep BP neural network model is established, the network structure is improved by increasingthe number of hidden layers of the neural network, the network calculation complexity is optimized, the food-borne disease epidemiological pathogenic factor accurate analysis and prediction network model is established, and real-time updating and overlapping are performed through the dynamic migration network with a self-learning function; the execution efficiency and sensitivity of the discrimination model network for food-borne disease pathogenic factor prediction are improved; and missing data is preprocessed, data containing missing items is reconstructed and analyzed, and the data is made to participate in effective network calculation.

The invention relates to a feed additive capable of preventing diseases and promoting growth, and a preparation method thereof, and belongs to the technical field of feed additive. The feed additive is composed of pine cone scale, dried orange peel powder, Andrographis paniculata Nees extract, purslane herb extract, motherwort extract, Artemisia argyi extract, scutellaria baicalensis root powder,and starch, is capable of clearing heat and detoxifying, enhancing metabolism, preventing bacteria, improving immunity of livestock, increasing the survival rate and daily weight gain rate of livestock, and inhibiting growth of mould and bacteria in forage. The raw materials of the feed additive are all common natural Chinese herbal medicines, are nontoxic and harmless for human and animals; the feed additive is used for replacing antibiotics in feed, so that mankind food borne diseases can be prevented effectively; the feed additive is safe and reliable, and is friendly to the environment.

The invention discloses enterobacter hormaechei bacteriophage YZU.P.A-5 and application thereof. The bacteriophage is identified to have unique genome structure characteristics, and belongs to a novelkind of bacteriophage; and the bacteriophage has been preserved in China Center for Type Culture Collection since June 15, 2020 under the preservation number of CCTCC NO: M 2020206. The bacteriophagedisclosed by the invention can efficiently crack enterobacter hormaechei so as to effectively inhibit enterobacter hormaechei in various matrices; and thus, the bacteriophage can be applied to preparation of a bacterial depressant for controlling enterobacter hormaechei pollution. The bacterial depressant prepared by the bacteriophage can effectively control growth of enterobacter hormaechei in culture media and food matrices, as well as effectively inhibit formation of enterobacter hormaechei mycoderm on surfaces of equipment, utensils and raw material solids; and moreover, the bacterial depressant is simple to prepare and convenient to use, as well as capable of reducing risks of enterobacter hormaechei propagation and food-borne diseases.

The invention especially relates to a coating flour for vegetarian diets and a manufacturing method thereof, belonging to the technical field of food processing. A recipe for the coating flour for vegetarian diets comprises the following raw materials by weight: 100 parts of modified starch, 5 to 20 parts of dried meat floss powder, 10 to 20 parts of egg yolk powder, 20 to 50 parts of chestnut powder, 0 to 20 parts of soybean powder and 1 to 5 parts of a compound seasoning. A recipe for the compound seasoning comprises the following components by weight: 10 parts of sucrose, 2 to 5 parts of table salt, 0.1 to 1 part of delicious amino acid and 0.1 to 0.5 part of sodium alginate. The dried meat floss powder is leftovers generated in processing of dried meat floss, i.e., broken dried meat floss powder. The coating flour for vegetarian diets provided by the invention is prepared from the dried meat floss processing leftovers i.e., broken dried meat floss powder, and accessory materials like egg yolk powder with thick and moist taste and the chestnut powder through procedures like baking, crushing and seasoning. In use, the coating flour is directly smeared onto the surface of a vegetarian diet or is immersed in and blended with a soupstock before coat the surface of the vegetarian diet, so a traditional vegetarian diet has strong meat diet flavor, the appetite of people is enhanced, the level of health is improved, and the occurrence of food-borne diseases caused by animal-derived food is reduced.

The invention relates to a food-borne disease outbreak identification method and system based on link prediction. The method comprises the following steps: performing data processing on food-borne disease outbreak event data; sampling the case data after data processing to obtain a positive and negative sample pair set; performing feature extraction on each positive and negative sample pair in the positive and negative sample pair set; inputting the positive and negative sample pairs after feature extraction into a link prediction model constructed based on a neural network to learn an association relationship between cases; constructing a case relationship network according to output of the link prediction model, wherein nodes in the network represent case entities, edges represent association relationships between cases, and edge weights reflect association strength between cases; and according to the case relationship network, adopting a community discovery algorithm to obtain a food-borne disease outbreak event. According to the method, a traditional clustering problem is converted into a problem of inter-case association relationship prediction and community discovery in a graph network, so that a better outbreak event recognition effect is achieved compared with traditional clustering algorithms.

The invention belongs to the field of microbial fermentation, and particularly relates to pediococcus pentosaceus derived from breast milk and application of the pediococcus pentosaceus. The pediococcus pentosaceus derived from the breast milk is pediococcus pentosaceus HM75-1 and is preserved in the China Center for Type Culture Collection on October 22, 2019, and the preservation number of the pediococcus pentosaceus is CCTCC NO: M 2019849. The strain is high in bacteriostatic ability, has relatively high inhibitory activity on common pathogenic bacteria in intestinal tracts, and particularly has a remarkable inhibitory effect on enterotoxigenic escherichia coli; the strain is good in gastrointestinal tolerance and high in adaptability to gastrointestinal tracts of human bodies, and canbe finally used for preparing medicines for inhibiting pathogenic bacteria of intestinal tracts and preventing and treating food-borne diseases, infant milk powder supplements, food preservatives andthe like.

The perforated line service pasta basket is fabricated from single-ply food service grade stainless steel. A steel sheet is heat-compressed into a cylindrical shape and provided with a single-ply perforated bottom wall. The bottom wall is raised to stimulate water circulation within the basket. A handle is provided on the basket to allow for manipulation of the basket to and from a pasta cooker. The use of stainless steel eliminates the possibility of rusting, thereby decreasing the chance of foodborne illness caused by contamination.

The invention relates to a pre-enrichment liquid culture medium for repairing cold and thermal injuries of Cronobacter. The pre-enrichment liquid culture medium comprises peptone, monopotassium phosphate, disodium hydrogen phosphate, sodium chloride, sucrose, sodium pyruvate, magnesium chloride and water. By verifying a repairing effect of the culture medium by virtue of a lot of food-borne pathogenic bacteria, results show that compared with existing pre-enrichment culture media such as BPW (Buffered Peptone Water), EE (Enterobacteria Enrichment Broth), TSB (Trypticase Soy Broth) and the like, the growth effect and repairing effects of physiological and biochemical indexes of the pre-enrichment liquid culture medium are greatly improved, the missing inspection defect of the Cronobacter in food inspection quarantine processes can be overcome, the detection ratio of the Cronobacter can be improved, and the growth and propagation of non-target bacteria such as salmonella and the like can be effectively restrained. The pre-enrichment liquid culture medium is suitable for rapidly repairing the Cronobacter in various foods and has important significances in the prevention and control of the occurrence and propagation of food-borne diseases caused by the food-borne Cronobacter infection.

The invention discloses a food poisoning source DNA bar code database and a rapid detection and identification tracing method thereof, and relates to the technical field of biological engineering. Themethod comprises the following steps of: 1, looking up literature and food poisoning data of past years to obtain the species data, regional distribution characteristics and morphological informationof fugu toxin fish, high biogenic amine aquatic products and toxic plants, including mushrooms, which affect food safety. According to the invention, on one hand, a DNA bar code technology which is oriented to food safety, effectively relates to classification, toxicity and morphology of toxic animals and plants is provided, on the other hand, rapid identification and application research on foods which may contain toxic animals and plants in the market of China is carried out so as to provide technical support for researching, judging and preventing food-borne disease outbreak events. A local pathogen spectrum is constructed through gene screening or full gene sequencing means, and a local molecular typing database is constructed through a molecular typing technology.

The invention discloses a food-borne disease information monitoring and early warning method and system, which are applied to a doctor client; the method comprise the following steps: acquiring patient diagnosis and treatment information input by a doctor; on the basis of a preset symptom keyword database, extracting symptom keywords in the diagnosis and treatment information; generating a food-borne disease report card based on the symptom keywords; performing integrity verification and logicality verification on the food-borne disease report card; and uploading the verified food-borne disease report card to a public health auditing client in a hospital.

The invention provides a maca tablet candy containing tagatose and a Maca tablet and belongs to the field of foods. In the invention, low-temperature-pulverized Maca ultra-micro powder is composed with tagatose of which the weight ratio is 4-50% and the purity is not less than 98%. The candy is free of addition of any fruit powder, traditional Chinese herbal medicines, saccharose-series high-calorie sweetening agents, sour agent, flavoring agent, excipient and the like food additives. For regulating the taste, the candy is added with a proper amount of stachyose. The candy is easy to absorb through oral mucosa for improving reproductive system functions. The candy has the effects of conditioning intestinal micro-ecological balance, enhancing the capability of resisting digestion canal infection or food-borne diseases by human body, improving nutrition absorption and enhancing immunity. The candy can enhance key blood factors, can improve blood health of human body, has the effect of resisting oxidization, is beneficial to prevention and treatment on various chronic diseases and is beneficial to people suffered from high blood pressure, high blood fat and high blood glucose and to the elders. The candy does not cause fat deposition, is more in metabolic energy consumption, is free of decayed teeth, is suitable for people suffered from high blood fat and obesity and for women, has the effect of resisting high blood glucose and is suitable for II-type diabetic patients whom have problem in intestine canal.

The invention discloses a staphylococcus aureus bacteriophage SapYZUalpha and application thereof, the staphylococcus aureus bacteriophage SapYZUalpha is identified to have unique genome structural characteristics, belongs to a novel bacteriophage and is preserved in the China Center for Type Culture Collection, the preservation time is January 5, 2022, and the preservation number is CCTCC NO: M2022023. The bacteriophage capable of efficiently cracking staphylococcus aureus can effectively inhibit staphylococcus aureus in various matrixes, and can be applied to preparation of a bacteriostatic agent for controlling staphylococcus aureus pollution. The bacteriostatic agent prepared by the invention can effectively control the growth of staphylococcus aureus in a culture medium and a food matrix, can effectively inhibit the formation of staphylococcus aureus pellicles on the surfaces of equipment, vessels and raw material solids, is simple to prepare and convenient to use, and can reduce the propagation of staphylococcus aureus and the risk of causing food-borne diseases.

The invention discloses an irradiation method for reducing mercury ion valence state in refrigerated livestock, poultry and fish, comprising the followed steps: a step 1, packaging original refrigerated livestock, poultry and fish containing Hg2+ residual of exceeding standard; a step 2, irradiating with 60 Co gamma ray or 50-10MeV electron beam ray, wherein the irradiation accumulated dose is 6-30 kGy, and the ununiformity of the radiation dose is less than 1.25; and a step 3, detecting the Hg2+ residual quantity of the original refrigerated livestock and poultry livestock, poultry and fish after irradiated in the step 2, repeating the step 2 if the detecting result is higher than the standard value until equal to or lower than the standard value. The method of the invention has a simple process, effectively reduces the Hg2+ residual in livestock, poultry and fish, and enhances foodstuff security; and simultaneously, the method can kill all or the great mass of microbe, and kill the pathogenic bacteria, thereby avoiding food borne diseases, further raising foodstuff health quality, and increasing foodstuff value.

Some HBL and NHE enterotoxins are known to cause food-borne diseases in humans. Enterotoxin-deficient mutants of member strains of the Bacillus cereus group that do not produce HBL, HBLa1, HBLa2, or NHE enterotoxins are disclosed. Enterotoxin-deficient mutants are suitable for use as biocontrol agents. Methods for making the mutants and for using the mutants are described.

The invention discloses a food-borne disease pathogenic factor prediction method based on big data, and the method comprises the following steps: S1, building a food-borne disease sample analysis database through the collection and arrangement of food-borne disease accident cases, recording the feature items contained in each sample, determining a training set and a test set, performing attributeselection and neuron definition, preprocessing missing data, and constructing a food-borne disease pathogenic factor deep BP neural network prediction model with relatively high accuracy. The method has the advantages that expert prediction data and deep BP neural network prediction are combined, the obtained prediction result is more accurate, acquisition and updating of prediction data are facilitated, dynamic prediction of food-borne diseases is achieved, the disease development trend is better predicted, a prediction scheme and measure adjustment are made, prevention, control and treatmentof diseases are facilitated, and the method is suitable for popularization and application. Based on big data, the prediction system is more perfect, and the timeliness and accuracy of disease prediction are improved.