The invention discloses a lucid ganoderma cultivation medium and a preparation method thereof. The lucid ganoderma cultivation medium is prepared from the following raw materials in parts by weight: 20-30 parts of elm branches, 18-28 parts of weed tree sawdust, 8-15 parts of bone meal, 10-15 parts of trichosanthes kirilowii Maxim shell, 5-10 parts of distillers dried grains with soluble, 4-8 parts of worm dung, 8-15 parts of cushaw stem, 5-10 parts of grape seed meal, 4-8 parts of marigold residues, 10-15 parts of bagasse, 0.3-0.6 part of monopotassium phosphate, 0.3-0.6 part of potassium nitrate, 0.3-0.5 part of calcium ammonium nitrate, 1-3 parts of discard molasses, 4-6 parts of plant ash, 1-3 parts of land plaster and 5-10 parts of nutrition additives. According to the cultivation medium disclosed by the invention, raw materials such as cushaw stem and grape seed meal are added on the basis of the traditional sawdust raw materials, cushaw stem and grape seed meal are abundant and are rich in various nutritive elements, and therefore the forest resource pressure is relieved. Meanwhile, the added nutrition additives are good for absorption and growth of lucid ganoderma, the utilization rate of the cultivation medium is improved, the biotransformation rate is increased, and the lucid ganoderma is wide, thick and solid in cover and strong in stem, rich in nutrients and high in medical property.