The present invention discloses biological extraction process for licorice, intermediate and end product made from main chemical components thereof. The process includes the following steps: crushing licorice into fine powder, enzymolysis, filtering and fermentation for full dissociation, centrifuging the fermented liquid to eliminate thallus, deactivating, chemically depositing to separate out coarse glycyrrhetic acid product, and purifying to obtain purified glycyrrhetic acid product. The coarse glycyrrhetic acid product and the purified glycyrrhetic acid product may be prepared into various kinds of medicine preparation. The present invention has the advantages of high yield of effective components, reduced waste slag, low cost, low power consumption and simple technique route, fully avoids environment pollution caused by excessive use of strong acid, strong base and harmful solvent, overcomes effective chemical component racemization, isomerization and structure rearrangement caused by vigrous chemical reaction.