A method for preparing nanometer porous pellumina which can regulate aperture and/or pitch of holes, which comprises the following steps: firstly, carrying out annealing and electrochemical polishing to an aluminum sheet, secondly, arranging the treated aluminum sheet in a constant-potential electrolytic cell, adding phosphorus acid solution as electrolyte, adding polyethylene glycol as adjusting agent, carrying out constant-potential anodic oxidation, then obtaining the nano-porous pellumina which can regulate aperture and/or pitch of holes, wherein the average molecular weight of polyethylene glycol is 400-2000, the mass percentage concentration n of the polyethylene glycol in the electrolyte is 0%<n<=50%, the concentration of the phosphorus acid in the electrolyte is 0.2-0.8 mole, and the electrolyzing time is 4-48h. Polyethylene glycol is adopted by the method as adjusting agent, effective adjusting to the aperture and the pitch of holes is realized, range of oxidizing electric potential is expanded through the chemical protection the electrochemical protection of polyethylene glycol, the used addition agent polyethylene glycol has the advantages of safety, environmental protection and low cost, therefore, the method is convenient to spread.