The invention discloses a method for making crispy and tender beef jerky and aims to provide a method for making beef jerky. The method comprises the following steps: cooking rinsed beef in boiling water, cutting into granules, and cooking the beef granules in marinade for 25-35 minutes; taking out the beef granules, mixing with auxiliary materials, and rolling in a rolling machine for three timesaccording to a negative pressure-normal pressure alternative manner, wherein forward and backward rotation is alternately performed during rolling each time; transferring the rolled beef granules into a baking tunnel oven, baking for 30 minutes, and immersing in sauce; quickly freezing under low-temperature conditions for 10 minutes after taking out, and transferring into the baking tunnel oven,and baking for 30 minutes again; immersing the beef granules baked twice into the sauce, quickly freezing under low-temperature conditions for 10 minutes after taking out, and transferring into a microwave oven, and baking for 60 minutes; and cooling, moisturizing and softening until the moisture content of the beef granules is 18%. The beef jerky made by the method disclosed by the invention is strong in fragrance, crispy and tender in taste, moderate in softness and hardness, delicious and mellow in taste, easy to chew, easy to melt, difficult to stick and suitable for people of all ages.