51results about How to "Synthetic preparation is simple" patented technology

The invention relates to six oligonucleotide sequences and an application thereof, relating to the recognition detection of vibrio alginolyticus. The invention provides six oligonucleotide sequences capable of inactivating the recognition detection of vibrio alginolyticus, a preparation method and an application thereof. The oligonucleotide sequence has the characteristics of being quick in detection, simple in operation, superior to that of antibody and the like in stability, and the preparation method is easy and relatively short in preparation period. The six oligonucleotide sequences comprise oligonucleotide sequences of SEQ ID No.1-6, and the recognition detection of vibrio alginolyticus can be inactivated by adopting one of the oligonucleotide sequences. The preparation method comprises the following steps: synthesizing an ssDNA oligonucleotide library used for filtering; carrying out SELEX filtering after the oligonucleotide library is mixed with inactivated vibrio alginolyticus till the affinity is not raised obviously; then cloning and sequencing; and selecting a highly copied ssDNA thereof to carry out verification of affinity specificity and determination of affinity constants so as to obtain corresponding an aptamer. The six oligonucleotide sequences can be used for recognition detection of inactivated vibrio alginolyticus.

The invention discloses three oligonucleotide sequences for identification and detection of vibrio alginolyticus as well as a preparation method and an application thereof and relates to identification and detection of vibrio alginolyticus. The oligonucleotide sequences comprise SEQ ID No. 1-3 (sequence identity number 1-3). The method comprises the following steps: synthesizing an ssDNA (single-stranded deoxyribonucleic acid) oligonucleotide library (5'-TCA GTC GCT TCG CCG TCT CCT TC----N35----GCA CAA GAG GGA GAC CCC AGA GGG-3') for screening; mixing the oligonucleotide library with the vibrio alginolyticus and then performing SELEX (systematic evolution of ligands by exponential enrichment) screening; performing cloning and sequencing on an aptamer-enhanced library after completing the SELEX screening; performing interception in different lengths on high-copy ssDNA emerged in the sequencing result so as to get a series of new sequences, and further constructing complementary sequences of the new sequences; and performing affinity and specificity verification on the obtained new sequences and the complementary sequences thereof so as to get corresponding aptamers.

The invention discloses a group of oligonucleotide sequences capable of synchronous identification of Vibrio harveyi and Vibrio alginolyticus and a preparation method of the oligonucleotide sequences, and relates to identification and detection of Vibrio harveyi and Vibrio alginolyticus. The oligonucleotide sequences comprise SEQ ID No.1-4. The preparation method comprises the following steps: synthesizing an ssDNA (single-stranded deoxyribonucleic acid) oligonucleotide library (5'-TCA GTC GCT TCG CCG TCT CCT TC----N35----GCA CAA GAG GGA GAC CCC AGA GGG-3') for screening; separately mixing the oligonucleotide library with Vibrio harveyi and Vibrio alginolyticus, and carrying out SELEX (systematic evolution of ligands by exponential enrichment) screening; cloning and sequencing the aptamer-rich library; and selecting high-copy ssDNA in the sequencing result, verifying specificity of affinity, and obtaining the corresponding aptamers.

The invention relates to a naphthalimide derivant containing 1, 3-dithiol-2-yliden conjugation units, a preparation method and application thereof. The chemical structure is shown in the specification, wherein when Pi-1 or Pi-2 is groups shown in the specification, R<1> or R<2> is a C1-30 alkyl (of which the formula is shown in the specification), and R<3> is H or alkyls of C1-4; when Pi-1 is equal to Pi-2 and further shown in the specification, the R<1> and the R<2> can not be alkyls at the same time; when the R<1> is equal to the R<2>, both the R<1> and the R<2> are alkyls. All variants of the derivant can be an n-type organic semi-conductor material when the derivant is used to prepare organic thin film transistor devices, and tests show that the electron mobility in the air can reach 0.2cm<2>V<-1>s<-1>.

The invention discloses a double-component crosslinking agent, a preparation method and applications thereof. The double component crosslinking agent comprises an acetylenic-bond-containing silane compound represented by the formula (I) and an azide-group-containing silane compound represented by the formula (II). The crosslinking can be achieved by heating the double-component crosslinking agent so as to trigger azide-alkyne cyclization reactions. Thus a crosslinked polymer insulation layer material can be prepared without any metal catalyst at a low temperature, and no by-product is generated during the process. The preparation technology is simple, and is suitable for various polymer materials such as polystyrene, polymethyl methacrylate, poly(p-vinylphenol), and the like. The obtained material is a potential transistor grid insulation layer material with an organic field effect, can be used to prepare various OFETs devices through a solution method, and has a very wide application prospect.

The invention provides an oligonucleotide sequence as well as a preparation method and application thereof, and relates to the identification and detection of inactivated Vibrio harveyi. The oligonucleotide sequence has the characteristics of quickness in detection, high simplicity in operation, higher stability than the stability of antibodies, simple preparation method, short preparation period, and so on. The oligonucleotide sequence is SEQ ID No. 1 which can be used to identify and detect the inactivated Vibrio harveyi. The preparation method comprises the following steps: synthesizing an ssDNA oligonucleotide library for screening; mixing the ssDNA oligonucleotide library with the inactivated Vibrio harveyi and screening the mixture by using the SELEX technology until the affinity no longer increases obviously; carrying out clone sequencing and selecting high copy ssDNA to undergo affinity and specificity verification and affinity constant determination, thereby obtaining the corresponding aptamer; The oligonucleotide sequence can be used to identify and detect the inactivated Vibrio harveyi in various samples.

The invention discloses a phosphorus-nitrogen-silicon-containing titanium dioxide hybrid nanoparticle flame retardant as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) reacting vanillic aldehyde, triethylamine and phosphorus oxychloride in a first solvent to obtain a product VP3; (2) enabling dichlorodiphenyl silane, K2CO3 and p-nitrophenol to react in a second solvent to obtain a product DPNSi, dissolving the DPNSi in a third solvent, then adding palladium-carbon super, and carrying out a reduction reaction with an ethanol solution of hydrazine hydrate to obtain DPASi; and (3) adding the VP3 and the DPASi into the ethyl acetate solution in which the titanium dioxide nanoparticles are dispersed, and carrying out condensationpolymerization reaction to obtain the titanium dioxide hybrid nanoparticle flame retardant VPASi containing phosphorus, nitrogen and silicon.

The invention discloses a preparation method and application of a dumbbell-shaped fluorine-containing polyphosphazene modified halloysite nanotube, and the dumbbell-shaped fluorine-containing polyphosphazene modified halloysite nanotube is designed and synthesized into a multifunctional hybrid flame retardant with a dumbbell-shaped special structure by utilizing the flame retardant effect of halloysite and fluorine-containing polyphosphazene with good thermal stability on a polymer material. The dumbbell-shaped fluorine-containing polyphosphazene modified halloysite nanotube has the advantagesas follows: raw materials are cheap and easily available; the synthesis and preparation are simple and easy; and with a fluorine-containing polyphosphazene polymer used as a pipe plug, an organic phosphine nitrogen system can fully play roles in capturing free radicals, inhibiting combustion and promoting charring and diluting gas during combustion.

The present invention relates to a pH-responsive fluorescence sensing material based on rhodamine B and cyanobiphenol, a preparation method and applications thereof, and belongs to the technical fieldof biochemical fluorescent sensing materials. According to the present invention, two pH-sensitive optical groups such as rhodamine B and cyanobiphenol are used base raw materials, wherein rhodamineB and hydrazine hydrate are subjected to a reaction to prepare rhodamine B hydrazide, and then the rhodamine B hydrazide and aldehyde-modified cyanobiphenol are combined through a Schiff base reactionto prepare the fluorescence sensing material; the method has advantages of mild preparation conditions and low raw material cost; the prepared material has good chemical stability, and has unique response properties to different pH values, wherein the responses can be presented through visible fluorescence and appearance color; and the prepared pH-responsive fluorescence sensing material can be used for the determination of the pH value in water and biological cells.

The invention discloses a kind of cyclopropane derivatives, and a preparation method and application thereof. Concretely, the cyclopropane derivatives have the structural formula X3 shown in the specification. In the formula, Ra, R1, R2, Rb and R4 are defined in the specification. The invention also provides a simple effective method for preparing the cyclopropane derivatives, raw material toxicity is low, and the obtained products are high in purity. The method is capable of preparing racemic compounds, and also is capable of preparing non-racemic compounds. The cyclopropane derivatives are further applicable to synthesize other cyclopropane derivatives.

The invention discloses a preparation method of an inorganic non-noble metal Ni doped Cu-based bifunctional electrocatalyst. The preparation method comprises the following steps: mixing copper sulfateand hydrochloric acid, and uniformly carrying out stirring to obtain a mixed solution; standing washed foamed nickel into the mixed solution of copper sulfate and hydrochloric acid under a room temperature condition; after the standing is finished, taking out the foamed nickel, rinsing the foamed nickel in absolute ethyl alcohol for a plurality of times, and then carrying out drying by cold air;putting the treated product in a KOH solution to carry out an in-situ electrochemical oxidation reaction, carrying out rinsing by deionized water for a plurality of times, and carrying out draying bycold air for later use; step four, putting the product in a KOH solution for carrying out an oxygen evolution reaction; preparing a sodium sulfide solution with a certain concentration for later use;standing the product obtained in the step four in the sodium sulfide solution for sulfur doping treatment; and after sulfur doping treatment, putting the product in a KOH solution to carry out a hydrogen evolution reaction. The method is simple, all the synthesis preparation processes are carried out at room temperature, excellent hydrogen evolution and oxygen evolution performance are both excellent, no noble metal participates in the processes, and the method is suitable for large-scale industrial production.

The invention discloses a selenium-containing pi extension naphthalene tetracarboxylic diimide compound, and a preparation method and an application thereof. The structure of the above naphthalene tetracarboxylic diimide derivative is represented as in the specification and claim, and is prepared through a reaction of 2,3,6,7-tetrahalogenonaphthalene tetracarboxylic diimide and 1,1-dicyanoethylene-2,2-diselenol or 1,1-dicyanoethylene-2,2-diselenol and 1,1-dicyanoethylene-2,2-dithiolate in an organic solvent. The naphthalene tetracarboxylic diimide derivative can be used as an organic thin film transistor of a semiconductor layer without heat treatment of the semiconductor film, the electron mobility of the derivative can reach 1.0cm<2>V<-1>s<-1>, the on/off ratio of the derivative is greater than 10<6>, the electron mobility of the monocrystalline field effect transistor device of the derivative an reach 2.0cm<2>V<-1>s<-1>, and the on/off ratio of the device is greater than 10<5>.

The invention relates to a preparation method of a low-carbon mixed gas hydrate explosion inhibitor, and can effectively solve the production problem of high-efficiency environmental-protection explosion-inhibition materials. The method comprises the steps: adding an inorganic salt into a solvent, making the mass content of the inorganic salt be 0.001-0.1 mol/L, and forming a hydration agent; mixing 5-15% of carbon dioxide and 85-95% of a nitrogen and argon mixed gas according to the volume ratio together, and forming an inert mixed gas; and filling the inert mixed gas into the hydration agent, making the pressure reach 4-10 MPa, carrying out a reaction at the temperature of -10 to 8 DEG C for 100-300 min to generate a mixed gas hydrate, crushing the mixed gas hydrate generated from the reaction into hydrate powder with the particle size of less than 1.0 mm at the temperature of -10 DEG C or less, and thus obtaining the product. The preparation method has the advantages of abundant rawmaterials, simple preparation method, easy synthesis and preparation, convenient use, low cost, cleaniness and environmental protection and good explosion suppression effect, is an innovation in theexplosion inhibitor, can effectively weaken energy required for the propagation of explosion pressure waves and hinder the propagation of explosion waves, and has huge economic and social benefits.

The invention discloses a non-noble metal copper-based catalyst and application thereof in benzylamine oxidative coupling reaction, the non-noble metal copper-based catalyst is obtained by loading a copper complex on a gamma-Al2O3 carrier, the molecular formula of the copper complex is [Cu2 (C17H14NP) 2Cl4], and the molecular formula of the non-noble metal copper-based catalyst is Cu2 (C17H14NP) 2Cl4/gamma-Al2O3, which is simply recorded as Cu2/gamma-Al2O3. The Cu2/gamma-Al2O3 catalyst can catalyze the oxidative coupling reaction of benzylamine under mild conditions, and meanwhile, the catalyst can be recycled for at least five times, the activity is kept unchanged, the substrate universality is relatively good, and the catalyst has very good practicability.

Disclosed are a monofluoro cyclopropane compound, and preparation method and uses thereof. The structure of the monofluoro cyclopropane compound is as represented by formula I, wherein R1, R2, Ra, and formula II are as defined in the specification. Also provided is a method for simply and effectively preparing the monofluoro cyclopropane compound. The method can prepare both racemic compounds and non-racemic compounds. The monofluoro cyclopropane compound can be further used to synthesize other cyclopropane derivatives.