The invention discloses a preparation technology of a natural organism and vegetable fiber microfiber material. The technology comprises the following steps: 1, preparing fiber batting composed of dermal fiber-containing composite fibers, modified high-shrinkage polyester fibers and vegetable fibers; 2, preparing a base cloth; 3, carrying out polyvinyl alcohol impregnation treatment; 4, carrying out PU impregnation treatment; 5, carrying out alkali decrement treatment; and 6, carrying out surface effect and function agent treatment: coating the surface of a semi-finished product to form a surface layer, and preparing the microfiber material from the surface layer according to a needed surface effect, wherein the surface layer is coated through the following steps: coating an air-permeable lining film, coating a surface slurry, drying the slurry, coating the surface slurry, and drying the slurry to obtain the finished microfiber material. The invention also discloses the microfiber material prepared through adopting the above technology, and a preparation technology of a product. The microfiber material and the product produced through the above technologies are comparable to derma in physical properties, and has the appearance, quality, strength, softness, air permeability and other physical properties of the derma.