The invention discloses a gasoline additive, which is characterized by being prepared from the following components by weight: 4-6 parts of a polyethylene glycol modified metal organic nano material, 30-40 parts of solvent oil, 2-4 parts of tetrahydroxy glycoluril, 3-5 parts of 2-benzyl tetrahydropyrrolo[3, 4-c]pyrrole-1, 3-dione, 1-2 parts of quaternary ammonium salt-15 modified polyether amine, 0.5-1 part of diphenylguanidine, and 4-8 parts of polyether amine. The invention also provides a preparation method of the gasoline additive and an application of the gasoline additive in gasoline. The gasoline additive provided by the invention can effectively improve the quality of gasoline, has an obvious oil-saving effect and multiple functions, and is environment-friendly and safe to use; gasoline can be promoted to be fully combusted and decomposed, the lubricity, cleanliness and corrosion resistance of an automobile are improved, power is enhanced, the service life of an engine is prolonged, automobile tail gas emission is reduced, and air and environment pollution is reduced.