The invention discloses a preparation method of a low-temperature curing water-based acrylic amino baking coating, belonging to the field of a production technology of a water-based amino baking coating. The method comprises the following steps of: mixing water, a wetting dispersant, an antifoaming agent, an anti-settling agent, a color filler, anticorrosion filler and water-based epoxy modified acrylic resin, and dispersing uniformly to obtain slurry; in a stirring state, adding water-based amino resin, an anti-flash rust agent, a drier and a leveling agent into the ground slurry; and dispersing uniformly by a high-speed dispersing machine. The water-based acrylic amino baking coating provided by the invention is convenient to produce, the product has a single component, the diluent is water, the cost is low, and the product is environment-friendly; and after being diluted, the water-based acrylic amino baking coating is directly subjected to spray construction, the construction is convenient, the curing temperature is low, the drying speed is high, the construction efficiency of the water-based acrylic amino baking coating is improved, the resources are saved, and the cost is lowered. The dried film is flat, full and bright, and has good gloss retention and color retention and excellent mechanical performance, water resistance and corrosion resistance; and the film is resistant to grease and high temperature.