The invention relates to an antibacterial environment-friendly board, particularly an antibacterial decorative board and a preparation method thereof. The antibacterial decorative board comprises, from top to bottom in order, an antibacterial surface layer, a decorative paper line layer, a balance enhancement layer, a substrate layer and a balance layer that are bonded to each other. The preparation method consists of: firstly making a stearic acid salt, calcium carbonate, carbon fiber and glass fiber, phenolic resin, unsaturated polyester resin, polyvinyl fluoride, copper naphthenate, cobalt iso-octoate, a coupling agent, and an acrylonitrile-butadiene-styrene copolymer into the substrate layer, pasting the balance enhancement layer and the balance layer respectively, performing pressing, then sticking the decorative paper line layer and conducting pressing, applying an antibacterial paint, and performing drying to form the antibacterial surface layer. The invention adopts a multilayer structure to ensure the board strength, the coating also can effectively prevent scratching, the balance enhancement layer and the balance layer improve the tensile properties of the board, and the substrate layer is made of a resin material, is elastic and can absorb the impact of external force, and the construction is convenient and fast.