The invention discloses a nematicide mixing device for preventing and controlling pine wood nematodes and a mixing method thereof, and belongs to the technical field of nematicide preparation. A nematicide mixing device for preventing and treating pine wood nematodes comprises a movable base and further comprises a vertical plate, a stirring device and a stirring device, the mixing box is connected to the movable base in a sliding manner; the motor is mounted on one side, close to the mixing box, of the vertical plate; according to the nematicide preparation device, powder agents and liquid agents can be simultaneously and successively added into the mixing box, the problem that in the prior art, mixing is not sufficient due to the fact that all the agents are added at a time is solved, the nematicide preparation quality is effectively improved, the mixing box can be shaken, gas can be sprayed into the mixing box at the same time, and the mixing efficiency is improved. Therefore, the powder medicament and the liquid medicament are more fully contacted and mixed, the mixing efficiency of the powder medicament and the liquid medicament is further improved, and the produced nematicide is better in quality, higher in practicability and suitable for popularization and use.