The invention belongs to the technical field of microscopy imaging, and discloses an integrated portable confocal opto-acoustic microscopy imaging device and a method. The imaging device comprises an opto-acoustic excitation light source, an acousto-optical confocal opto-acoustic detector, a photodiode, a miniature X-Y two-dimensional galvanometer, a spectroscope, a lens, a sample table, a double-channel parallel acquisition card and a computer with acquisition control software and two-dimensional galvanometer control software. The method includes steps that after the direction of laser light emitted from the opto-acoustic excitation light source is changed via the miniature X-Y two-dimensional galvanometer, the laser light is focused by the balsaming lens and irradiates on samples to excite opto-acoustic signals, the opto-acoustic signals are received by the acousto-optical confocal opto-acoustic detector, the incident angle of a light beam is continuously changed by the two-dimensional galvanometer so that the samples are scanned, and finally, an opto-acoustic image is rebuilt by maximum projection algorithm. A micro-chip laser device and the miniature X-Y two-dimensional galvanometer are integrated to form the portable confocal opto-acoustic microscopy imaging device, mobility is good, imaging speed is fast, and resolution ratio and contrast ratio are high.