The invention discloses a pomegranate seed protein powder feed for accelerating fish growth and weight gain and increasing the feed conversion rate and a preparation method thereof. The pomegranate seed protein powder feed is prepared from the raw materials of, by weight, 1200-1500 parts of pomegranate seeds, 1-1.6 parts of sodium hydroxide, 2-3 parts of hydrochloric acid, 0.1-0.15 part of a compound protease, 3-5 parts of calcium hydrophosphate, 25-27 parts of taro leaves, 17-18 parts of alfalfa, 43-46 parts of soybean meal, 23-25 parts of peanut meal, 7-8 parts of sesame dregs, 3-4 parts of Palmaria palmata, 5-6 parts of scirpus tabernaemontani, 0.1-0.15 part of a lactic acid bacteria starter, 0.3-0.5 part of costustoot powder, 0.1-0.2 part of a sea-buckthorn extract, 0.3-0.5 part of chicken grease, 0.7-0.8 part of tricholoma gambosum powder, 1.2-1.5 parts of kiwi fruit powder, 5-6 parts of red bean protein powder and 2-3 parts of an ox bone extract. By adoption of the pomegranate seed protein powder feed for accelerating fish growth and weight gain and increasing the feed conversion rate, fish growth and weight gain can be accelerated, the feed conversion rate can be increased, the fish culture cost is lowered, and the anti-virus and antibacterial capability of fishes can be improved.