The invention discloses a preparation method for 
peptide chelated calcium of 
clanis bilineata tsingtauica 
larva. The preparation method comprises the following steps: 
degreasing the 
clanis bilineata tsingtauica 
larva and then carrying out superfine crushing, adding water and uniformly mixing, and adjusting the pH value to 2-9; adding 
protease in the obtained mixed liquid, stirring for 
enzymatic hydrolysis, then inactivating the 
protease to obtain a crude enzymatic 
hydrolysate, and centrifuging to obtain an supernatant liquid; taking the obtained supernatant liquid and removing impurities to obtain a purified liquid, and carrying out separation and purification by virtue of at least one 
ultrafiltration membrane with a molecular weight 
cut-off of 1-50kD to obtain enzymatic hydtolysis 
peptide components with different molecular weights; adding a 
calcium source, and chelating to obtain a crude 
peptide chelated calcium product of the 
clanis bilineata tsingtauica 
larva; and collecting precipitates after concentration and 
precipitation, and 
drying to obtain the peptide 
chelated calcium product of the clanis bilineata tsingtauica larva. According to the method disclosed by the invention, a method of classifying through the 
ultrafiltration membranes with different molecular weight 
cut-off at first, and then chelating is adopted for the enzymatic product, on one hand, the technical 
bottleneck that 
a peptide calcium chelate is difficult to separate is solved; and on the other hand, the peptide chelated 
calcium products with different molecular weights of the clanis bilineata tsingtauica larva are prepared and can be used by people with different needs, thus the use efficiency of the products is increased, and the pertinence of the products is improved.