The invention discloses feed capable of remarkably improving disease resistance of a pangolin and a preparation method of the feed. The feed is prepared by proportionally mixing termite powder, shrimp shell powder, rice, black locust leaf powder, cooked egg yolks, milk powder, pomegranate barks, Indian kalimeris herbs, merremia boisiana, Chinese mosla herbs, oxalis corymbosa, compound vitamin, compound microelements, glucose, hydrolyzed protein and miramycin, wherein the compound vitamin comprises vitamin B, vitamin A, vitamin E and vitamin D, and the compound microelements comprise iron, copper, zinc, manganese, selenium, iodine and tin. The feed is excellent in taste, low in preparation cost and rich and reasonable in nutrition, the autoimmunity of the pangolin can be enhanced while growth and development of the pangolin are promoted, the disease resistance of the pangolin is enhanced, the scale and shell quality and the meat quality of the pangolin are ensured, common diseases of the pangolin in the artificial breeding process can be actively treated, the breeding risk of the pangolin is remarkably reduced, and economic benefits are improved.