The invention discloses a waterproof environment-friendly packaging bag and a preparation method, the waterproof environment-friendly packaging bag comprises a base layer, and the base layer comprises the following main components: water, rice husk, cellulose, starch, natural resin, lignin, a curing agent, a phosphate ceramic material and biodegradable plastic. The base layer is prepared from the rice husks, the cellulose, the starch, the natural resin, the lignin, the curing agent and other materials, so that the environment-friendly effect of the base layer can be greatly improved, and the base layer has a degradable function on the basis of improving the strength of the base layer; meanwhile, the phosphate ceramic material and the biodegradable plastic are wide in degradation range, can effectively improve the wear-resistant and waterproof effects of the packaging bag, are non-toxic to the environment, enable the base layer to form a scientific beneficial substance proportion, and solve the problems that an existing industrial transportation packaging bag is poor in waterproof effect, prone to being affected by external moisture in the use process and poor in waterproof performance. The sealing protection effect of the packaging bag is seriously disturbed, and the use requirement of a high-humidity environment cannot be met.