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107results about How to "Preparation science" patented technology

Sample preparation and treatment method for researching metaproteomics of soil

The invention discloses a sample preparation and treatment method for researching the metaproteomics of soil. The method comprises the following steps: pretreating a soil sample to obtain a first suspension; carrying out concussion crushing to obtain a crushed first suspension; carrying out ultrasonic treatment, centrifuging the treated suspension, and collecting the obtained first supernatant; precipitating the first supernatant with cold acetone to obtain a protein solution; precisely weighing the protein solution containing proteins, adding a single protease or a protease combination, and incubating the proteins overnight to obtain a polypeptide solution; adding a separating agent 1, carrying out vortex treatment, adding a separating agent 2, carrying out vortex treatment, centrifuging the obtained solution, standing the centrifuged solution, and transferring about 90% of the obtained lower layer phase of a polypeptide-containing segment into a new centrifuging tube; adding a separating agent 3, carrying out vortex treatment to realize uniform mixing, centrifuging the treated solution, and taking the obtained lower layer; mixing the above obtained two peptide segment solution, desalting the obtained solution mixture, and collecting the obtained eluate; and centrifuging, concentrating and drying the eluate, afresh dissolving the obtained peptide segment sample, and directly injecting the dissolved sample to a machine for analysis. The method has the advantages of simplicity in operation, high stability, high recovery rate, completeness in extraction, and high flux, and is of great significance to massively develop the relevant researches of the metaproteomics of soil.
Owner:赛思莱(厦门)科技有限公司

Sample preparation treatment method for excrement and soil metabonomics study

The invention discloses a sample preparation treatment method for excrement and soil metabonomics study. The sample preparation treatment method comprises the following steps: pretreating a sample to obtain first suspension; adding a steel ball and shaking, breaking and extracting to obtain the broken first suspension; performing ultrasonic treatment; centrifuging; collecting first supernate and precipitate; concentrating the first supernate until the first supernate is nearly dry; adding water-soluble re-dissolution liquid and a steel ball, and shaking for re-dissolution; centrifuging at a high speed, wherein obtained second supernate is a water-soluble metabolism extract which can be analyzed directly on a machine; adding a fat-soluble metabolite extracting solution into the collected precipitate, and mixing uniformly to obtain second suspension; shaking, breaking and extracting; performing ultrasonic treatment; centrifuging; collecting a lower-layer solution; blowing to dry the obtained lower-layer solution through a nitrogen blowing way; adding fat-soluble re-dissolution liquid and a steel ball, and shaking for re-dissolution; centrifuging at a high speed, wherein an obtained product is a fat-soluble metabolism extract which can be analyzed directly on the machine. The method disclosed by the invention has the advantages of simple operation, strong stability, comprehensiveness and high recovery rate, and is of great significance to extensive related studies of excrement and soil metabonomics.
Owner:赛思莱(厦门)科技有限公司

Low temperature antique glaze with cleaner air effect and preparing method thereof

The invention discloses a low temperature antique glaze with cleaner air effect and preparing method thereof, the preparing method comprises the steps of: A, preparing low temperature frit; B, preparing glaze, mixing low temperature frit, colorant, potassium feldspar, quartz, calcite, talc, calcium phosphate, anion compounds and antibacterial composite to obtain the mixing powder, putting sodium tripolyphosphate and carboxymethyl cellulose into powder and mixing the powder evenly, fine grinding, and then adding water so as to obtain the glaze; C, applying the glaze evenly on the body, and then putting the body into the reducing atmosphere furnace, cooling the natural glaze to room temperature after heat preservation, and the low temperature antique glaze with cleaner air effect is obtained. Compared with the prior antique glaze, the antique glaze produced by the invention has the advantages of scientific ingredients,reasonable preparing method, pure color and stable performance; at the same time with a reasonable combination of negative ions and antibacterial composite materials, the synergistic action of the two making the antique glaze has characteristics of excellent anti-bacterial, anti-fouling and air purification, the scope of the application of the antique glaze is further broaden.
Owner:佛山市高明区诚睿基科技有限公司

Two-stage broiler compound feed and preparing method thereof

The invention discloses two-stage broiler compound feed and a preparing method thereof. The compound feed comprises 0-21-day-old feed and 22-45-day-old feed. The two-stage broiler compound feed and the preparing method thereof have the advantages that matching is scientific, preparation is easy, feed cost is reduced, the nutritional requirement for broiler growth and development can be met, the utilization rate of feed is increased, and broiler growth is promoted; by the adoption of traditional Chinese medicine additives, the spleen and stomach can be strengthened, the appetite and digestion can be promoted, normal nutrient intake of broilers can be guaranteed on the premise that feed cost is reduced, and high broiler production performance is realized.
Owner:SHANDONG NEW HOPE LIUHE GROUP

Intelligent method for moisture retention spraying curing of concrete through coupling of temperature, humidity and wind speed

ActiveCN107759247AMaintain humidityScientific realization of moisturizing and maintenanceWater-power plantsHydro energy generationCouplingStop time
The invention provides an intelligent method for moisture retention spraying curing of concrete through the coupling of temperature, humidity and wind speed to scientifically realize moisture retention curing of concrete. The intelligent method is characterized by comprising the following steps: 1, arranging a protective layer on the surface of concrete, and selecting and arranging a spraying device; 2, arranging an environment data real-time acquisition system to acquire air temperature, air humidity and wind speed in real time; 3, carrying out intelligent spraying curing: 3-1, determining aspraying time Tk; 3-2, calculating a spraying stop time Tb, receiving the air temperature, the air humidity and the wind speed acquired in the step 2 by a computer, and calculating the spraying stop time Tb based on the air temperature, the air humidity and the wind speed, wherein the minimum value of calculation results in a formula 1 and a formula 2 is selected as the spraying stop time Tb; 3-3,carrying out cyclic curing: adopting computer to control the spraying device to spray according to the spraying time Tk, and control the spraying device to stop spraying according to the spraying stop time Tb, wherein the cyclic curing is carried out continuously until a curing age is reached.
Owner:WUHAN UNIV

Low-temperature archaized glaze and preparation method thereof

The invention discloses low-temperature archaized glaze and a preparation method thereof. The preparation method comprises the following steps: Step A, preparing a low-temperature frit; Step B, preparing glaze slip: mixing the low-temperature frit, a colouring agent, potash feldspar, quartz, calcite, talcum, calcium phosphate, a deodorant, a negative ion compound and an antibacterial compound to obtain mixed powder, adding sodium tripolyphosphate and carboxymethyl cellulose into the mixed powder and uniformly mixing, grinding, and adding water to obtain the glaze slip; and Step C, uniformly applying the glaze slip onto a green body, putting the green body into a reducing atmosphere furnace, carrying out thermal insulation and naturally cooling to room temperature so as to obtain the low-temperature archaized glaze. In comparison with existing archaized glaze, the manufactured archaized glaze in the invention has advantages of scientific batching, reasonable preparation, pure color and stable performance. Through reasonable cooperation of the deodorant, the negative ion compound and the antibacterial composite material, the archaized glaze has excellent antibacterial antifouling and air purification characteristics. According to the invention, the application range of the archaized glaze is further widened.
Owner:佛山市高明区诚睿基科技有限公司

Photochromic ceramic ink capable of being sunken and used for ceramic ink jet and preparing method thereof

The invention discloses a photochromic ceramic ink capable of being sunken and used for ceramic ink jet and a preparing method thereof. The preparing method comprises the steps of A, preparing a subsidence glaze; B, measuring the subsidence glaze, a dispersing agent, a surface additive, an antifoaming agent, a binding agent and the balance solvent according to a ratio, and grinding the mixture to obtain an ink semi-finished product; C, filtering the ink semi-finished product to obtain an ink finished product. Compared with a prior ceramic ink capable of being sunken, the photochromic ceramic ink capable of being sunken is used cooperatively with a pigment ink, which can show concavo-convex and lifelike textures of ceramic tiles more fully and vividly, have a better third dimension, further improve ornamental effects of the ceramic tiles, create colorful patterns, and can be widely applied to fields of ceramic chips, archaized bricks and the like; meanwhile through reasonable matching of photochromic compounds and antibiosis composite materials with the ceramic ink, the ceramic ink has excellent antibacterial, antifouling and photochromic characters, which further broadens applied range of the ceramic ink capable of being sunken.
Owner:FOSHAN GAOMING HAIDI CERAMIC RAW MATERIAL CO LTD

No-outdoor unit air conditioner for pollution purification and novel refrigeration

The invention discloses a no-outdoor unit air conditioner for pollution purification and novel refrigeration. The no-outdoor unit air conditioner is formed by sleeving a cylindrical upper air purifying section, a cylindrical middle fan section and a lower air conditioner condensation section in sequence. The no-outdoor unit air conditioner is characterized in that the lower air conditioner condensation section is provided an upper evaporator coil II and an upper evaporator coil I at the upper part, a refrigerant storage tank at the middle part and a lower evaporator coil at the lower part; a heating device shell is arranged at the external of the right side; and an electric heating element and a power supply wire are arranged in the heating device shell. An absorbent nanogel comprises the following components in proportion by weight: 10 g of pectin, 2 g of agar, 3 g of menthol, 5 g of eugenol, and 0.01 g of nanometer zinc. The no-outdoor unit air conditioner for pollution purification and novel refrigeration breaks through the difficulty of easy generation of pollution in traditional window air conditioners, cabinet air conditioners, hanging air conditioners and central air conditioner chamber evaporation pipes, is low in size, electricity-saved and low in noise, in particular, needs no air conditioner purifying filter screen, and is excellent in purifying effect.
Owner:GANZHOU ANHONG ENVIRONMENTAL PROTECTION TECH CO LTD

Deodorizing antibacterial low-temperature archaized glaze and preparation method thereof

The invention discloses deodorizing antibacterial low-temperature archaized glaze and a preparation method thereof. The preparation method comprises the following steps: Step A, preparing a deodorizing antibacterial low-temperature frit; Step B, preparing glaze slip: mixing the low-temperature frit, a colouring agent, potash feldspar, quartz, calcite, talcum, calcium phosphate, a deodorant and an antibacterial compound to obtain mixed powder, adding sodium tripolyphosphate and carboxymethyl cellulose into the mixed powder and uniformly mixing, grinding and adding water so as to obtain the glaze slip; and Step C, uniformly applying the glaze slip onto a green body, putting the green body into a reducing atmosphere furnace, carrying out thermal insulation and naturally cooling to room temperature so as to obtain the deodorizing antibacterial low-temperature archaized glaze. In comparison with existing archaized glaze, the manufactured archaized glaze in the invention has advantages of scientific batching, reasonable preparation, pure color and stable performance, and also has lasting antibacterial property, antifouling self-cleaning function and deodorizing property. According to the invention, the application range of the archaized glaze is further widened.
Owner:佛山市高明区诚睿基科技有限公司

Method for testing rut of rigid-flexible composite pavement structure

ActiveCN107860665AEvaluation of high temperature anti-rutting performanceGood anti-rutting performance at high temperatureMaterial strength using tensile/compressive forcesTriaxial shear testSurface layer
The invention discloses a method for testing a rut of a rigid-flexible composite pavement structure. A test is carried out through a Hamburg rut instrument, a test piece is a composite test piece andis placed in a test mold of the Hamburg rut instrument; the composite test piece consists of an asphalt concrete surface layer, a cement concrete panel and a composite material in sequence from top tobottom, an adhesive layer is arranged between the asphalt concrete surface layer and the cement concrete panel, and the test piece is formed from bottom to top; the composite material is composed ofrubber powder, sand and broken stones with a single particle size, vibration compaction is adopted to form the composite material, a dynamic triaxial test is used to determine a rebound modulus of thecomposite material, and the proportion of the rubber powder, the sand and the broken stones with a single particle size is determined by whether 95-105% of a required rebound modulus value is reachedor not; and after a normal test is started, the test is ended after the rut depth reaches 20mm. The testing method provided by the invention can provide a scientific basis for improving the rutting resistance design and durability of the rigid-flexible composite pavement structure.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Antibacterial ceramic inkjet ink with marble crack effect and method for preparing antibacterial ceramic inkjet ink

The invention discloses antibacterial ceramic inkjet ink with a marble crack effect and a method for preparing the antibacterial ceramic inkjet ink. The method includes steps of A, mixing and stirring solvents, hyper-dispersants, regulators, defoaming agents and anti-diffusion agents with one another to obtain mixed solution, adding 0.1-5% antibacterial compounds into the mixed solution and uniformly stirring the antibacterial compounds and the mixed solution; B, adding permeating powder and photochromic compounds into the mixed solution, grinding the permeating powder, the photochromic compounds and the mixed solution, then adding solvents and anion compounds into the mixed solution and grinding the solvents, the anion compounds and the mixed solution to obtain semi-finished ink products; C, filtering the semi-finished ink products to obtain finished ink products. The antibacterial ceramic inkjet ink and the method have the advantages that as compared with existing ceramic ink, the antibacterial ceramic inkjet ink manufactured by the aid of the method contains scientific ingredients, is stable in performance and has grains, colors and textures of natural marble, decoration effects of ceramic tiles further can be improved, and the antibacterial ceramic inkjet ink further can be widely applied to the fields of ceramic chips, antique bricks and the like; the photochromic compounds, the anion compounds and antibacterial composite materials are reasonably matched with one another, accordingly, the antibacterial ceramic inkjet ink is excellent in antibacterial performance, air purification and photochromic characteristic, and the application range of the antibacterial ceramic inkjet ink further can be broadened.
Owner:FOSHAN GAOMING HAIDI CERAMIC RAW MATERIAL CO LTD
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