68 results about "Bacterium bifidum" patented technology

The invention provides a compound probiotic for relieving anxiety behaviors and application of the compound probiotic. The compound probiotic comprises bifidobacterium animalis YRK-12 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.28597) and lactobacillus rhamnosus LRa-Y8 (the preservation number is CGMCC NO.34186). The invention finds that the two bacteria are compounded and can be matched and promoted with each other, and have a synergistic effect on the aspect of preventing and treating anxiety disorder, which is specifically shown as follows: the reduction of center lattice movement time and percentage caused by LPS (Lipopolysaccharide) is relieved; the increase of mouse serum proinflammatory cytokines caused by LPS is improved; the increase of mouse brain tissue proinflammatory cytokines caused by LPS is improved. Under the condition that the using amount of the bacteria is consistent, the effect of the compounding of the two bacteria on improving the anxiety-like behavior induced by the LPS is obviously improved. Meanwhile, the two bacteria are probiotics, so that the product is safe and is not easy to generate resistance.

The invention belongs to the technical field of fermentation engineering and enzyme engineering, and particularly relates to a method for producing gamma-aminobutyric acid at high yield through mixed fermentation. Specifically, a mixed fermentation mode is adopted, the growth of lactobacillus hilgardii is promoted through the growth of bifidobacteria, and the synergistic interaction of the two strains is realized, so that the yield of GABA is greatly improved. Experimental results show that compared with traditional single-bacterium fermentation, the mixed fermentation method has the advantages that the yield of the GABA can be increased by 2-3 times, the fermentation period is shortened by 50% or above, and remarkable technical advantages and economic benefits are achieved. Therefore, the mixed fermentation method provided by the technical scheme provides an efficient and environment-friendly new way for the production of high-yield GABA, and has important application value and market prospect.

The invention belongs to the technical field of food processing, and particularly relates to fermented beef jerky and a preparation method thereof. The preparation method of the beef jerky comprises the following steps: (1) removing fascia and fat from fresh cattle hind leg meat, washing with clear water, and cutting into meat slices; (2) adding table salt, glucose, white granulated sugar and sodium nitrite into the sliced meat, uniformly mixing, adding a fermentation inoculant which comprises staphylococcus calcaratus, pediococcus acidilactici, bifidobacterium bifidum and Witzia ciliaris, and fermenting; and (3) after fermentation, drying and cooling to obtain the fermented beef jerky. Specific strains are selected to form a fermentation inoculant for fermentation, and the prepared fermented beef jerky is high in free amino acid and soluble peptide content, low in nitrite residual quantity, uniform red brown in color and slightly bright in surface, and belongs to high-quality beef jerky.

The invention relates to the technical field of functional food production through microbial fermentation, in particular to a herba epimedii enzymolysis probiotic fermentation refined extract and a preparation method thereof.The preparation method comprises the following steps that herba epimedii and beta-cyclodextrin are mixed, an obtained mixed solution is subjected to enzymolysis treatment through pectinase, cellulase and glycosidase and then serves as a solid-state fermentation substrate, and the herba epimedii enzymolysis probiotic fermentation refined extract is obtained; adding mixed bacteria of lactobacillus casei, bifidobacterium lactis and lactobacillus gasseri, performing solid-state fermentation, and performing vacuum freeze drying to obtain a herba epimedii probiotic fermentation refined extract; the herba epimedii probiotic fermentation refined extract is prepared by adopting an enzymolysis fermentation method in cooperation with a beta-cyclodextrin inclusion technology, the extraction efficiency, the solubility, the biological activity, the stability and other performance of effective components in herba epimedii are improved, the preparation method is simple, and operation is easy and convenient; the used materials are edible, green and safe; the process saves energy consumption, has good environmental benefits, greatly saves manpower and material resources, and can be applied to industrial large-scale production.

The present invention provides an oral liquid for reducing fat and promoting metabolism and its preparation process, belonging to the technical field of oral liquid preparation; the preparation process includes the following steps: preparation of cistanche juice; preparation of compound fruit vinegar juice; preparation of oligosaccharides from tiger nut meal; preparation of compound concentrated juice of prune and noni fruit; preparation of oral liquid for reducing fat and promoting metabolism. The present invention inhibits the activity of fat synthase through compound fruit vinegar juice to reduce fat synthesis; oligosaccharides from tiger nut meal promote the growth and reproduction of beneficial bacteria such as Bifidobacterium and Lactobacillus to assist in reducing fat, and the compounding of prune and noni fruit exerts efforts simultaneously from two aspects of inhibiting fat synthesis and promoting fat decomposition, more effectively reducing the accumulation of body fat, fundamentally solving the problem of fat accumulation, and effectively achieving the effect of reducing fat and promoting metabolism through the synergistic effect of multiple components from multiple angles.

The invention provides ginseng freeze-dried powder capable of resisting oxidation, improving immunity and inhibiting cancer cell diffusion and a preparation method of the ginseng freeze-dried powder, and belongs to the technical field of ginseng processing. According to the ginseng freeze-dried powder researched and developed by the invention, three traditional Chinese medicinal materials including ginseng, lucid ganoderma and radix ophiopogonis are selected, the performance is improved through synergistic fermentation of bifidobacterium bifidum CICC-6168 and lactobacillus helveticus CICC-6273, then the ginseng freeze-dried powder is prepared by utilizing a vacuum freeze-drying technology, and the prepared product has the effects of resisting oxidation, improving immunity, inhibiting cancer cell diffusion and reducing uric acid.

The application provides a fattening late-stage pig feed and a preparation method thereof, and relates to the technical field of pork breeding. The fattening late-stage pig feed is characterized by adding 10-15% of a nutritional supplement to per kilogram of fattening feed, and the nutritional supplement is composed of pine branch fermentation material, lotus root fermentation material, alfalfa powder, roxburgh rose extract and caffeine. The pine branch fermentation material is obtained by subjecting pine branches to cellulase enzymolysis treatment and then to fermentation by means of bifidobacterium and spencerolyn cryptococcus. The lotus root fermentation material is obtained by subjecting cooked lotus roots to fermentation by means of lactobacillus plantarum. The application overcomes the shortcomings of the prior art, effectively guarantees the weight gain efficiency of porkers in the late fattening stage, further improves pork quality, and comprehensively improves the economic benefits of pork breeding.

The invention relates to a probiotic composition for promoting alcohol degradation and metabolism and improving discomfort symptoms after drinking. The probiotic composition comprises the following raw materials in parts by weight: 35-40 parts of lactitol; 28 to 35 parts of fructo-oligosaccharide; 15 to 34 parts of stachyose; 5-10 parts of mango fruit powder; 2 to 4 parts of lactobacillus rhamnosus LR519; 0.5 to 2.5 parts of plant lactobacillus LP45 (Lipoprotein 45); 0.005 to 0.015 part of bifidobacterium bifidum B11, and 0.005 to 0.015 part of bacillus subtilis; 0.005 to 0.015 part of Lactobacillus helveticus L551, and 0.005 to 0.015 part of Lactobacillus And 0.1 to 0.3 part of vitamin C. The traditional Chinese medicine composition can promote alcohol degradation, accelerate alcohol metabolism and effectively improve discomfort symptoms after drinking.

The invention discloses a biological preparation suitable for young pigeon growth and a preparation method and application thereof, and belongs to the technical field of feeds, the biological preparation comprises the following components by weight: 8-40 parts of a microbial agent, 1-10 parts of an additive, 1-10 parts of an antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite; the microbial agent comprises bifidobacterium animalis, lactobacillus acidophilus, lactobacillus paracasei and lactobacillus reuteri; the additive comprises at least one of yeast beta-glucan and astragalus polysaccharide. According to the biological preparation suitable for young pigeon growth, montmorillonite is used as a base material, under the combined action of the microbial inoculum, the additive, the antioxidant and peptidoglycan, the biological preparation capable of adjusting intestinal health of breeding pigeons and improving the immunity of the breeding pigeons is obtained, meanwhile, immune factors in pigeon milk can be improved, and the growth of young pigeons is promoted. The effects of improving the growth performance of the young pigeons and reducing the elimination rate of the breeding pigeons and the young pigeons can be achieved, so that economic benefits are improved, and wide application prospects are achieved.

The application provides a coffee peel enzyme fermentation agent and a preparation method of the enzyme, and belongs to the technical field of enzyme production.The preparation method of the coffee peel enzyme comprises the following steps: mixing coffee peel powder and sugar of sweetener oil residue, and sequentially performing fermentation of Saccharomyces cerevisiae YNXC003 and anaerobic fermentation of Bifidobacterium bifidum YNXC004 to obtain the coffee peel enzyme.The coffee peel powder and the sugar of sweetener oil residue are used as composite raw materials, special bacterial agents are combined, and a segmented fermentation mode is adopted, so that the nutritional activity of the two raw materials can be fully released, the nutritional level and flavor taste of the enzyme can be significantly improved, and the enzyme can be endowed with physiological functions such as blood sugar reduction.The total acid content of the coffee peel enzyme is 8-12 g / L, the total phenol content is 1.5-2.5 g / L, the coffee peel enzyme is rich in bioactive substances such as short-chain fatty acids, polyphenol compounds and amino acids, and has a significant blood sugar reduction effect.

The application relates to the field of food, in particular to zero-cane normal-temperature yoghourt and a preparation method thereof. The zero-cane normal-temperature yoghourt comprises the following raw materials in parts by weight: raw cow milk or concentrated milk 80-90 parts, protein powder 0.4-3 parts, cheese 0.3-2 parts, sweetening agent 0.01-15 parts, stabilizer 0.1-5 parts and bacterial strain 50-200 U, the bacterial strain is composed of lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus and lactobacillus bifidus, wherein the sum of lactobacillus bulgaricus and streptococcus thermophilus: the sum of lactobacillus acidophilus and lactobacillus bifidus = 9:1-8:2. The application adopts raw cow milk or concentrated milk, protein powder and cheese to form a basic system in a specific proportion, and is fermented by specific bacterial strains, so that the zero-cane yoghourt can reach a fermentation end point within 8 hours, and the product has a good taste.

The invention relates to the technical field of bean product processing, in particular to a preparation method of fermented probiotic fortified dried bean curds.The preparation method comprises the steps that soybeans and black beans serve as raw materials, mixed soybean milk is prepared through soaking, cleaning and pulping, lactic acid bacteria and bifidobacterium leavening agents are inoculated, the pH and the temperature are adjusted for primary fermentation, then fermented soybean milk is obtained through heat treatment and filtration, and the fermented soybean milk is obtained; the preparation method comprises the following steps: preparing microcapsules containing active bifidobacteria from sodium alginate-chitosan, heating fermented soybean milk, curdling with brine, pressing into bean curd blanks, soaking with functional components, smearing with a probiotic microcapsule suspension, carrying out vacuum drying, soaking with seasoning liquid, carrying out hot air drying, carrying out surface smearing with liquid for air drying, and carrying out vacuum packaging and sterilization to obtain a finished product. According to the process, fermentation and probiotic microcapsule technologies are combined, functional characteristics are given to the product, the digestibility and bioavailability of soybean protein are improved through lactobacillus fermentation, various functional peptides with physiological activity are generated, and the nutritional value of the product is remarkably improved.

The invention provides a sugarcane fermentation product, a food quality guarantee product, a preparation method and application, and particularly belongs to the technical field of food preservation. The preparation method of the sugarcane leavening comprises the following steps: crushing sugarcane, and performing first sterilization to obtain sterile sugarcane pulp; inoculating the compound bacteria into the sterile sugarcane pulp for fermentation; the compound bacteria comprise lactic acid bacteria, bifidobacterium bifidum and propionibacterium; a degerming compound enzyme is supplemented in the fermentation process; the compound enzyme comprises xylanase, sucrase, phytase and aminopeptidase; and after fermentation, carrying out secondary sterilization, filtering, and discarding filter residues to obtain the sugarcane fermentation product. The sugarcane leavening disclosed by the invention is prepared from natural raw materials, has good anti-corrosion and anti-oxidation effects, and can prolong the shelf life of food.

The pet meat staple food grain based on composite prebiotic fermentation and the preparation method thereof have the advantages that 1, a composite prebiotic combination adopts a synergistic proportion of fructo-oligosaccharide, inulin, stachyose and galactooligosaccharide, and the regulation efficiency of intestinal flora is remarkably improved through the design that the proportion of a GF2 component is greater than or equal to 70% and the proportion of a long-chain component (the polymerization degree is 6-8) is greater than or equal to 20%; 2, animal meat sources (cattle tendon and chicken breast) and fermented plant raw materials are scientifically proportioned, and high-bioavailability protein and soluble fiber are provided; 3, the probiotic preparation is compounded by lactobacillus acidophilus and bifidobacterium in a ratio of 1: 1.5, and the survival rate of viable bacteria and the yield of metabolites are ensured in combination with a staged fermentation process; and 4, the mineral premix and the vitamin compound are accurately added, so that the nutritional requirements of pets in different life cycles are met, and the synergy of nutrition comprehensiveness and functionality is realized.

The present invention discloses a co - culture of Bifidobacterium bifidum and macrophages, a preparation method and an application, belonging to the field of biotechnology. The co - culture of Bifidobacterium bifidum and macrophages of the present invention is the product after co - culturing Bifidobacterium bifidum BD - 1 and macrophages. A preparation method of the co - culture of Bifidobacterium bifidum and macrophages disclosed by the present invention comprises the following steps: co - culture the bacterial suspension of Bifidobacterium bifidum BD - 1 and the macrophage suspension, then centrifuge and take the supernatant to obtain the product. The present invention discloses the application of the above co - culture in the preparation of products for promoting the growth and development of nerve cells. The co - culture of the present invention has an obvious promoting effect on the development indexes such as mRNA expression and protein translation of nerve cells, and can promote the morphological development of nerve cells.

The invention relates to the technical field of agricultural product deep processing, in particular to a deep processing method for improving Chinese yam activity. Comprising the steps of Chinese yam crushing, Chinese yam puffing treatment and Chinese yam fermentation treatment. Wherein strains adopted in the Chinese yam fermentation treatment are lactobacillus plantarum CCFM1137 and bifidobacterium animalis B420; the production process is simple, the comprehensive utilization degree of the Chinese yam is high, and the raw material cost is low; according to the present invention, after the puffing treatment and the specific probiotic fermentation, the determination on the activity of the product finds that compared with the puffed Chinese yam or fermented Chinese yam product, the anti-inflammatory and anti-oxidation activity is significantly improved, and the low dose and the high effect are achieved.

A method of treating, preventing or ameliorating at least one symptom of obsessive compulsive disorder (OCD) in a subject is disclosed. The method comprises a step in which a multistrain probiotic formulation is administered to the subject. The multistrain probiotic formulation includes the bacterial strains Bacillus coagulans, Lactobacillus rhamnosus, Bifidobacterium lactis, Lactobacillus plantarum, Bifidobacterium breve and Bifidobacterium infantis and Glutamine in a concentration range of 100-500 mg.

The invention discloses a primer composition for detecting bifidobacterium bifidum of which the microbial preservation number is CCTCC (China Center for Type Culture Collection) NO: M2023349, enterococcus avium of which the microbial preservation number is CCTCC NO: M2023350, lactobacillus salivarius of which the microbial preservation number is CCTCC NO: M2023348, lactobacillus fermentum of which the microbial preservation number is CCTCC NO: M2023352, and parabacteroides didysonii of which the microbial preservation number is CCTCC NO: M20222033. The invention further discloses a kit based on the primer composition and application of the kit. The primer composition has good specificity and high accuracy aiming at the five bacteria, and can detect the five bacteria in a complex environment to evaluate the physiological status of a subject.

The invention relates to a rice fermentation product filtrate, a preparation method thereof and a hair care composition containing the rice fermentation product filtrate, and the preparation method of the rice fermentation product filtrate comprises the following steps: crushing rice, gelatinizing and saccharifying to obtain a culture medium, inoculating bifidobacterium, lactobacillus and lactic acid bacteria into the culture medium, and naturally fermenting to obtain the rice fermentation product filtrate. Sterilizing the filtrate to obtain a unique rice fermentation product filtrate, and highly concentrating and enriching nutritional ingredients without any dilution; besides, the rice fermentation product filtrate prepared by the method is compounded with the citrus chachiensis extract and the ginger oil, the three components play a synergistic effect, and the obtained hair care composition can remarkably improve the tensile strength and luster of hair, reduce combing force, relieve scalp itching and relieve inflammation.

The invention belongs to the technical field of extraction of effective components of medicines, and particularly relates to a composition containing citrus polyphenol and citrus powder for burning fat and losing weight as well as a preparation method and application of the composition. The composition comprises spray-dried powder and compound probiotics, wherein the spray-dried powder is prepared from citrus fruit filtrate, flammulina velutipes fibers, soybean protein isolate, an aronia extract and a black tea extract, and the compound probiotics comprise lactobacillus acidophilus, lactobacillus plantarum, lactobacillus rhamnosus, bifidobacterium lactis and lactobacillus casei. The flammulina velutipes fiber, the soybean protein isolate, the aronia extract, the black tea extract and the citrus fruit filtrate are subjected to compound spraying, the stability of total polyphenol and Vc in citrus fruits is improved, the obtained citrus powder is further mixed with compound probiotics to prepare the composition with pharmacodynamic active ingredients, fat can be effectively combusted, weight can be effectively reduced, and the health-care effect is good. The compound can be applied to weight-losing medicines or health products.

The invention belongs to the field of medicine, and particularly relates to a montmorillonite-containing medicine compound and a preparation method thereof.The preparation method comprises the steps that cultured bifidobacterium bifidum bacterial liquid and cultured lactobacillus helveticus bacterial liquid are subjected to high-speed centrifugation, bacterial sludge is collected, the bifidobacterium bifidum bacterial sludge, the lactobacillus helveticus bacterial sludge, montmorillonite and trehalose are weighed, water is added, the materials are mixed to be uniform, and the montmorillonite-containing medicine compound is prepared. Adding 1%-2% of a sodium alginate solution, and uniformly stirring and mixing to prepare a suspension; the preparation method comprises the following steps: adding a chitosan-acetic acid solution with the pH value of 3.5 into a chitosan-acetic acid solution in an extruding and dropwise adding manner, filtering, adding into a carboxymethyl chitosan solution, assembling pellets for 4-6 times, and drying in a fluidized bed at 50-70 DEG C, thereby obtaining the product. The montmorillonite and the probiotics are combined for use, the montmorillonite can adsorb harmful substances which are not beneficial to the growth of the probiotics in the intestinal inflammation environment, the staying time of the probiotics in the intestinal tract is prolonged, the colonization rate is increased, and the probiotics relieve the adverse reactions such as constipation and intestinal flora imbalance occurring in the process of taking the montmorillonite.

The invention discloses a preparation method of a double-strain synergistic fermentation antihypertensive beverage, and particularly relates to the technical field of brewing beverage preparation. The double-strain synergistic fermentation antihypertensive beverage comprises the following raw materials in parts by weight: 1-2 parts of blue thistle pollen, 10-15 parts of a dogbane leaf decocted preparation, 5-15 parts of a pomegranate flower decocted preparation, 10-15 parts of a rose decocted preparation, 10 parts of a coreopsis bicolor decocted preparation, 2-5% of fructo-oligosaccharide, a viable count ratio of bifidobacterium longum 1714 to lactobacillus plantarum CW006 being (1-2): 1, and the balance of water. According to the preparation method of the double-strain synergistic fermentation antihypertensive beverage disclosed by the invention, four breakthroughs are realized through double-strain synergistic fermentation: the viable count reaches 1.2 * 10 < 8 > CFU / mL, and the ACE inhibition rate is 89.2%, so that the industry is new and high; the anthocyanin retention rate is 82%, the stability double guarantee is formed in the 45-day shelf life, and the centrifugal stability is 2.3% and is superior to the national standard by 5 times; the fermentation period is shortened to 14 hours, the yield reaches 98.7% in cooperation with real-time biosensing monitoring, the double-bacterium symbiotic index SI is equal to 1.32, and the yield of gamma-aminobutyric acid is increased by 61%.

The invention relates to the technical field of hemp plant disease and insect pest control, in particular to a hemp plant disease and insect pest biological control fungicide and a preparation method thereof. The invention provides a biological control fungicide for plant diseases and insect pests of cannabis sativa plants. The microbial agent is prepared from the following components in parts by mass: 10 to 15 parts of bifidobacterium bifidum, 12 to 16 parts of paenibacillus polymyxa, 16 to 20 parts of bacillus amyloliquefaciens, 12 to 16 parts of burkholderia, 13 to 17 parts of pythium oligandrum, 6 to 10 parts of lactobacillus plantarum, 15 to 19 parts of bacillus velezensis, 15 to 19 parts of pseudomonas aeruginosa and 12 to 16 parts of metarhizium anisopliae. The biological control fungicide for the plant diseases and insect pests of the cannabis sativa has a good control effect on various plant diseases and insect pests of the cannabis sativa, has a remarkable growth promoting effect on the cannabis sativa when being used as a cannabis sativa seed dressing agent, and has a good application prospect.

The invention discloses a method for reducing the estrogen-like effect of royal jelly and improving the biological activity at the same time, royal jelly is used as a fermentation raw material, lactobacillus plantarum, lactobacillus casei and bifidobacterium are sequentially used as fermentation strains, the fermentation raw material is sequentially fermented, the estrogen-like activity in the royal jelly is effectively reduced, and the biological activity is improved at the same time. The lactobacillus plantarum, the lactobacillus casei, the streptococcus thermophilus and the bifidobacterium are sequentially inoculated, so that the reduction process of the estrogen content can be accelerated, and the reduction speed is obviously higher than that of single-bacterium fermentation and simultaneous fermentation. According to the optimization process, the fermentation efficiency is remarkably improved, the fermentation process is accelerated, the blood sugar reducing activity, the anti-tumor activity and the anti-oxidation activity of the royal jelly are further improved, the flavor is improved, and the applicable crowd and the application scene of the royal jelly are expanded.

The invention belongs to the technical field of dairy products, and particularly relates to high-water-retention solidified yogurt as well as a preparation method and application thereof. The preparation raw materials of the set yogurt with high water-retaining property comprise raw milk, a cheese product, concentrated whey protein powder, yolk powder and a leavening agent. The leavening agent comprises lactobacillus delbrueckii subsp. Bulgaricus, streptococcus salivarius subsp. Thermophilus, bifidobacterium animalis subsp. Lactis and lactobacillus acidophilus. The cheese product, the concentrated whey protein powder and the yolk powder are taken as core functional raw materials and form a synergistic effect, so that the yoghourt has good water-retaining property, sensory evaluation and storage stability. Meanwhile, by compounding the four strains, the water-retaining property, the storage stability, the taste, the tissue state and the like of the yoghourt can be improved.

The present invention relates to the technical field of natural extracts, and specifically to a camphor seed kernel polysaccharide, a preparation method thereof, and an application thereof. The camphor seed kernel polysaccharide provided in the present application comprises the following monosaccharides: glucose, mannose, galactose, arabinose, and glucosamine hydrochloride, and the molar ratio is (0.88-0.92): (0.06-0.1): (0.015-0.02): (0.003-0.004): (0.003-0.004); the weight-average molecular weight of the camphor seed kernel polysaccharide is 1000Da-20000Da. The present invention also provides a preparation method for the above-mentioned camphor seed kernel polysaccharide and an application of the polysaccharide. The results showed that camphor seed kernel polysaccharides CCSKP and CCSKP1‑A had good in vitro digestibility, and could promote the growth and reproduction of Lactobacillus acidophilus, Bifidobacterium longum, Bifidobacterium infantis and Bifidobacterium adolescentis, and produce corresponding SCFAs. They had potential prebiotic activity, which was beneficial to improving the comprehensive utilization efficiency and added value of camphor seed resources.

Provided is a simpler method that can be applied to food and that increases the production amount of exopolysaccharides. The present invention provides a method for producing an exopolysaccharide, comprising: a step for treating an exopolysaccharide-producing bacterium under conditions in which the retardation period is prolonged by 10% or more; and a step for culturing the treated exopolysaccharide-producing bacterium in a culture medium to produce an exopolysaccharide. The present invention provides a composition containing any kind of lactic acid bacteria from among a bacterium belonging to Lactobacillus delbrueckii having an exopolysaccharide production capacity of 30 mg / kg or more, a bacterium belonging to Streptococcus thermophilus having an exopolysaccharide production capacity of 69 mg / kg or more, and a bacterium belonging to Bifidobacterium breve having an exopolysaccharide production capacity of 4.2 mg / kg or more.

The invention provides a livestock and poultry manure fermentation refined organic fertilizer, and belongs to the technical field of bio-organic fertilizers. A three-stage fermentation process is adopted, firstly, a mixed microbial agent containing microorganisms such as bacillus licheniformis, methanosarcina and bifidobacterium animalis is constructed, high-temperature aerobic fermentation is carried out on the mixed microbial agent and original livestock and poultry manure, and after a primary degradation effect is achieved, the microbial agent is supplemented to carry out normal-temperature aerobic fermentation; on the basis, a fermentation product is diluted, the pH is adjusted, then enzymolysis is conducted, protein is preliminarily hydrolyzed into small molecule peptide, meanwhile, polysaccharide is hydrolyzed into monosaccharide to be utilized by microorganisms, then lactobacillus paracasei, pediococcus pentosaceus, propionibacterium freudenreichii, mucor rouxii and absidia coerulea are inoculated, third-stage mixed fermentation is conducted, and fermentation liquid is obtained; and drying the fermentation product under reduced pressure to obtain a finished product. The fertilizer effectively improves the fertilizer efficiency, can obviously promote the growth of crops, is low in ammonia nitrogen content and sulfide content, and is safe and non-toxic.

The invention relates to the technical field of microorganisms, and particularly discloses a method for reducing viable count reduction of a viable bacterium product containing probiotics during storage, a complex microbial inoculant and application of the complex microbial inoculant. According to the method for reducing the reduction of the viable count of the product containing the viable probiotics during storage, the complex microbial inoculants are added into the product; the lactobacillus paracasei comprises lactobacillus paracasei PC-01 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.17537), animal bifidobacterium subsp. Lactis MN-Gup (the preservation number is CGMCC No.15578), plant lactobacillus Lp-6 (the preservation number is CGMCC No.16661), animal bifidobacterium subsp. Lactis M8 (the preservation number is CGMCC No.18610) and lactobacillus casei Zhang (the preservation number is CGMCC No.5469). The complex microbial inoculant provided by the invention can delay the reduction of the survival rate of viable bacteria when a product containing the complex microbial inoculant is stored, and the product added with the complex microbial inoculant can have an excellent bowel relaxing effect.

The invention relates to the technical field of biological environmental protection, and discloses a compound microbial agent for constructing micro-ecological balance based on five florae. Comprising the following raw materials in parts by weight: 3-5 parts of photosynthetic bacteria, 1-2 parts of lactic acid bacteria, 2-4 parts of yeast acid, 2-5 parts of bacillus, 2-5 parts of actinomycetes, 1-2 parts of acetic bacteria, 2-3 parts of trichoderma viride, 2-4 parts of bifidobacterium, 2-3 parts of lactobacillus plantarum, 1-2 parts of nitrifying spina bacteria, 10-14 parts of corn, 10-14 parts of soybeans, 10-14 parts of sorghum and 1-3 parts of aspergillus. According to the compound microbial agent with micro-ecological balance constructed based on the five flora, the aspergillus is decomposed into monosaccharide by utilizing energy, the monosaccharide becomes food of saccharomycetes, the monosaccharide is converted into alcohol after being subjected to saccharomycetes bacterial decomposition, the alcohol is converted into acetic acid by acetic acid bacteria, and carbon dioxide generated by the saccharomycetes is utilized by photosynthetic bacteria to prepare glucose; the microbiota complement each other to live together, and micro-ecological balance is constructed through the five microbiota.