A preparation method of a traditional Chinese medicine microecological feed additive for layers at early period of laying comprises the steps of taking traditional Chinese medicines, i.e., herba dandelion, common andrographis herb, radix isatidis, herba motherwort, herba purple common perilla, radix scutellariae, eucommia ulmoides leaves, astragalus root, pilose asiabell root and licorice root, performing superfine grinding on the traditional Chinese medicines and adding ethanol solution to boil through high heat, preserving temperature with gentle heat, cooling, filtering, concentrating filter liquor, adding white sugar to boil, cooling to room temperature, then inoculating lactobacillus, tea fungus and bifidobacterium, fermenting until a white fungus block grows, and concentrating bacteria solution at low temperature; then weighing modified starch, Arabic gum, cyclodextrin and syrup, adding into the concentrated bacteria solution, stirring while adding, homogenizing at high temperature after mixing and stirring uniformly, and performing spray drying to obtain the traditional Chinese medicine microecological feed additive. The traditional Chinese medicine microecological feed additive has the efficacies of increasing beneficial bacteria in intestine, improving immunity, stress resistance and disease resistance of chicken body, and increasing egg yield. Compared with the control group, the laying rate is increased by 4.68% and 5.72%, the egg-feed ratio is decreased by 5.28% and 7.56%, a rate of eggs with soft shells and shell cracks is decreased by 32.17% and 22.46%, the feed is saved, costs are reduced, and economic benefits are increased.