36 results about "Bacterium bifidum" patented technology

The invention provides ginseng freeze-dried powder capable of resisting oxidation, improving immunity and inhibiting cancer cell diffusion and a preparation method of the ginseng freeze-dried powder, and belongs to the technical field of ginseng processing. According to the ginseng freeze-dried powder researched and developed by the invention, three traditional Chinese medicinal materials including ginseng, lucid ganoderma and radix ophiopogonis are selected, the performance is improved through synergistic fermentation of bifidobacterium bifidum CICC-6168 and lactobacillus helveticus CICC-6273, then the ginseng freeze-dried powder is prepared by utilizing a vacuum freeze-drying technology, and the prepared product has the effects of resisting oxidation, improving immunity, inhibiting cancer cell diffusion and reducing uric acid.

The application provides a fattening late-stage pig feed and a preparation method thereof, and relates to the technical field of pork breeding. The fattening late-stage pig feed is characterized by adding 10-15% of a nutritional supplement to per kilogram of fattening feed, and the nutritional supplement is composed of pine branch fermentation material, lotus root fermentation material, alfalfa powder, roxburgh rose extract and caffeine. The pine branch fermentation material is obtained by subjecting pine branches to cellulase enzymolysis treatment and then to fermentation by means of bifidobacterium and spencerolyn cryptococcus. The lotus root fermentation material is obtained by subjecting cooked lotus roots to fermentation by means of lactobacillus plantarum. The application overcomes the shortcomings of the prior art, effectively guarantees the weight gain efficiency of porkers in the late fattening stage, further improves pork quality, and comprehensively improves the economic benefits of pork breeding.

The invention discloses a biological preparation suitable for young pigeon growth and a preparation method and application thereof, and belongs to the technical field of feeds, the biological preparation comprises the following components by weight: 8-40 parts of a microbial agent, 1-10 parts of an additive, 1-10 parts of an antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite; the microbial agent comprises bifidobacterium animalis, lactobacillus acidophilus, lactobacillus paracasei and lactobacillus reuteri; the additive comprises at least one of yeast beta-glucan and astragalus polysaccharide. According to the biological preparation suitable for young pigeon growth, montmorillonite is used as a base material, under the combined action of the microbial inoculum, the additive, the antioxidant and peptidoglycan, the biological preparation capable of adjusting intestinal health of breeding pigeons and improving the immunity of the breeding pigeons is obtained, meanwhile, immune factors in pigeon milk can be improved, and the growth of young pigeons is promoted. The effects of improving the growth performance of the young pigeons and reducing the elimination rate of the breeding pigeons and the young pigeons can be achieved, so that economic benefits are improved, and wide application prospects are achieved.

The application provides a coffee peel enzyme fermentation agent and a preparation method of the enzyme, and belongs to the technical field of enzyme production.The preparation method of the coffee peel enzyme comprises the following steps: mixing coffee peel powder and sugar of sweetener oil residue, and sequentially performing fermentation of Saccharomyces cerevisiae YNXC003 and anaerobic fermentation of Bifidobacterium bifidum YNXC004 to obtain the coffee peel enzyme.The coffee peel powder and the sugar of sweetener oil residue are used as composite raw materials, special bacterial agents are combined, and a segmented fermentation mode is adopted, so that the nutritional activity of the two raw materials can be fully released, the nutritional level and flavor taste of the enzyme can be significantly improved, and the enzyme can be endowed with physiological functions such as blood sugar reduction.The total acid content of the coffee peel enzyme is 8-12 g / L, the total phenol content is 1.5-2.5 g / L, the coffee peel enzyme is rich in bioactive substances such as short-chain fatty acids, polyphenol compounds and amino acids, and has a significant blood sugar reduction effect.

The application relates to the field of food, in particular to zero-cane normal-temperature yoghourt and a preparation method thereof. The zero-cane normal-temperature yoghourt comprises the following raw materials in parts by weight: raw cow milk or concentrated milk 80-90 parts, protein powder 0.4-3 parts, cheese 0.3-2 parts, sweetening agent 0.01-15 parts, stabilizer 0.1-5 parts and bacterial strain 50-200 U, the bacterial strain is composed of lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus and lactobacillus bifidus, wherein the sum of lactobacillus bulgaricus and streptococcus thermophilus: the sum of lactobacillus acidophilus and lactobacillus bifidus = 9:1-8:2. The application adopts raw cow milk or concentrated milk, protein powder and cheese to form a basic system in a specific proportion, and is fermented by specific bacterial strains, so that the zero-cane yoghourt can reach a fermentation end point within 8 hours, and the product has a good taste.

The invention relates to the technical field of bean product processing, in particular to a preparation method of fermented probiotic fortified dried bean curds.The preparation method comprises the steps that soybeans and black beans serve as raw materials, mixed soybean milk is prepared through soaking, cleaning and pulping, lactic acid bacteria and bifidobacterium leavening agents are inoculated, the pH and the temperature are adjusted for primary fermentation, then fermented soybean milk is obtained through heat treatment and filtration, and the fermented soybean milk is obtained; the preparation method comprises the following steps: preparing microcapsules containing active bifidobacteria from sodium alginate-chitosan, heating fermented soybean milk, curdling with brine, pressing into bean curd blanks, soaking with functional components, smearing with a probiotic microcapsule suspension, carrying out vacuum drying, soaking with seasoning liquid, carrying out hot air drying, carrying out surface smearing with liquid for air drying, and carrying out vacuum packaging and sterilization to obtain a finished product. According to the process, fermentation and probiotic microcapsule technologies are combined, functional characteristics are given to the product, the digestibility and bioavailability of soybean protein are improved through lactobacillus fermentation, various functional peptides with physiological activity are generated, and the nutritional value of the product is remarkably improved.

The pet meat staple food grain based on composite prebiotic fermentation and the preparation method thereof have the advantages that 1, a composite prebiotic combination adopts a synergistic proportion of fructo-oligosaccharide, inulin, stachyose and galactooligosaccharide, and the regulation efficiency of intestinal flora is remarkably improved through the design that the proportion of a GF2 component is greater than or equal to 70% and the proportion of a long-chain component (the polymerization degree is 6-8) is greater than or equal to 20%; 2, animal meat sources (cattle tendon and chicken breast) and fermented plant raw materials are scientifically proportioned, and high-bioavailability protein and soluble fiber are provided; 3, the probiotic preparation is compounded by lactobacillus acidophilus and bifidobacterium in a ratio of 1: 1.5, and the survival rate of viable bacteria and the yield of metabolites are ensured in combination with a staged fermentation process; and 4, the mineral premix and the vitamin compound are accurately added, so that the nutritional requirements of pets in different life cycles are met, and the synergy of nutrition comprehensiveness and functionality is realized.

A method of treating, preventing or ameliorating at least one symptom of obsessive compulsive disorder (OCD) in a subject is disclosed. The method comprises a step in which a multistrain probiotic formulation is administered to the subject. The multistrain probiotic formulation includes the bacterial strains Bacillus coagulans, Lactobacillus rhamnosus, Bifidobacterium lactis, Lactobacillus plantarum, Bifidobacterium breve and Bifidobacterium infantis and Glutamine in a concentration range of 100-500 mg.

The invention relates to a rice fermentation product filtrate, a preparation method thereof and a hair care composition containing the rice fermentation product filtrate, and the preparation method of the rice fermentation product filtrate comprises the following steps: crushing rice, gelatinizing and saccharifying to obtain a culture medium, inoculating bifidobacterium, lactobacillus and lactic acid bacteria into the culture medium, and naturally fermenting to obtain the rice fermentation product filtrate. Sterilizing the filtrate to obtain a unique rice fermentation product filtrate, and highly concentrating and enriching nutritional ingredients without any dilution; besides, the rice fermentation product filtrate prepared by the method is compounded with the citrus chachiensis extract and the ginger oil, the three components play a synergistic effect, and the obtained hair care composition can remarkably improve the tensile strength and luster of hair, reduce combing force, relieve scalp itching and relieve inflammation.

The invention discloses a preparation method of a double-strain synergistic fermentation antihypertensive beverage, and particularly relates to the technical field of brewing beverage preparation. The double-strain synergistic fermentation antihypertensive beverage comprises the following raw materials in parts by weight: 1-2 parts of blue thistle pollen, 10-15 parts of a dogbane leaf decocted preparation, 5-15 parts of a pomegranate flower decocted preparation, 10-15 parts of a rose decocted preparation, 10 parts of a coreopsis bicolor decocted preparation, 2-5% of fructo-oligosaccharide, a viable count ratio of bifidobacterium longum 1714 to lactobacillus plantarum CW006 being (1-2): 1, and the balance of water. According to the preparation method of the double-strain synergistic fermentation antihypertensive beverage disclosed by the invention, four breakthroughs are realized through double-strain synergistic fermentation: the viable count reaches 1.2 * 10 < 8 > CFU / mL, and the ACE inhibition rate is 89.2%, so that the industry is new and high; the anthocyanin retention rate is 82%, the stability double guarantee is formed in the 45-day shelf life, and the centrifugal stability is 2.3% and is superior to the national standard by 5 times; the fermentation period is shortened to 14 hours, the yield reaches 98.7% in cooperation with real-time biosensing monitoring, the double-bacterium symbiotic index SI is equal to 1.32, and the yield of gamma-aminobutyric acid is increased by 61%.

The invention belongs to the technical field of dairy products, and particularly relates to high-water-retention solidified yogurt as well as a preparation method and application thereof. The preparation raw materials of the set yogurt with high water-retaining property comprise raw milk, a cheese product, concentrated whey protein powder, yolk powder and a leavening agent. The leavening agent comprises lactobacillus delbrueckii subsp. Bulgaricus, streptococcus salivarius subsp. Thermophilus, bifidobacterium animalis subsp. Lactis and lactobacillus acidophilus. The cheese product, the concentrated whey protein powder and the yolk powder are taken as core functional raw materials and form a synergistic effect, so that the yoghourt has good water-retaining property, sensory evaluation and storage stability. Meanwhile, by compounding the four strains, the water-retaining property, the storage stability, the taste, the tissue state and the like of the yoghourt can be improved.

Provided is a simpler method that can be applied to food and that increases the production amount of exopolysaccharides. The present invention provides a method for producing an exopolysaccharide, comprising: a step for treating an exopolysaccharide-producing bacterium under conditions in which the retardation period is prolonged by 10% or more; and a step for culturing the treated exopolysaccharide-producing bacterium in a culture medium to produce an exopolysaccharide. The present invention provides a composition containing any kind of lactic acid bacteria from among a bacterium belonging to Lactobacillus delbrueckii having an exopolysaccharide production capacity of 30 mg / kg or more, a bacterium belonging to Streptococcus thermophilus having an exopolysaccharide production capacity of 69 mg / kg or more, and a bacterium belonging to Bifidobacterium breve having an exopolysaccharide production capacity of 4.2 mg / kg or more.

The invention relates to the technical field of microorganisms, and particularly discloses a method for reducing viable count reduction of a viable bacterium product containing probiotics during storage, a complex microbial inoculant and application of the complex microbial inoculant. According to the method for reducing the reduction of the viable count of the product containing the viable probiotics during storage, the complex microbial inoculants are added into the product; the lactobacillus paracasei comprises lactobacillus paracasei PC-01 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.17537), animal bifidobacterium subsp. Lactis MN-Gup (the preservation number is CGMCC No.15578), plant lactobacillus Lp-6 (the preservation number is CGMCC No.16661), animal bifidobacterium subsp. Lactis M8 (the preservation number is CGMCC No.18610) and lactobacillus casei Zhang (the preservation number is CGMCC No.5469). The complex microbial inoculant provided by the invention can delay the reduction of the survival rate of viable bacteria when a product containing the complex microbial inoculant is stored, and the product added with the complex microbial inoculant can have an excellent bowel relaxing effect.

The invention relates to the technical field of biological environmental protection, and discloses a compound microbial agent for constructing micro-ecological balance based on five florae. Comprising the following raw materials in parts by weight: 3-5 parts of photosynthetic bacteria, 1-2 parts of lactic acid bacteria, 2-4 parts of yeast acid, 2-5 parts of bacillus, 2-5 parts of actinomycetes, 1-2 parts of acetic bacteria, 2-3 parts of trichoderma viride, 2-4 parts of bifidobacterium, 2-3 parts of lactobacillus plantarum, 1-2 parts of nitrifying spina bacteria, 10-14 parts of corn, 10-14 parts of soybeans, 10-14 parts of sorghum and 1-3 parts of aspergillus. According to the compound microbial agent with micro-ecological balance constructed based on the five flora, the aspergillus is decomposed into monosaccharide by utilizing energy, the monosaccharide becomes food of saccharomycetes, the monosaccharide is converted into alcohol after being subjected to saccharomycetes bacterial decomposition, the alcohol is converted into acetic acid by acetic acid bacteria, and carbon dioxide generated by the saccharomycetes is utilized by photosynthetic bacteria to prepare glucose; the microbiota complement each other to live together, and micro-ecological balance is constructed through the five microbiota.

The invention discloses a method for degrading African swine fever viruses by fermenting pennisetum sinese with compound probiotics, which comprises the following steps: crushing a pennisetum sinese raw material polluted by the African swine fever viruses; the preparation method comprises the following steps: inoculating a compound probiotic leavening agent containing six specific strains (pichia membranaefaciens C200-1, brevibacillus reuteri RF10-5, pediococcus acidilactici, lactobacillus plantarum, lactobacillus buchneri and bifidobacterium), and fermenting for 6-14 days at 20-40 DEG C under a closed condition. By means of the fermentation process, polluted African swine fever viruses in materials can be effectively degraded, and q-PCR detection shows negative on the seventh day. The method is specially used for solving the problem of African swine fever virus pollution, is clear in effect, green, safe and easy to implement, and provides a feed link solution for prevention and control of African swine fever.

The application discloses a kind of whole plant sweet potato bio-fermentation feed for pig and a preparation method thereof.The application comprises the following steps: bifidobacterium, Lactobacillus, Lactobacillus and lactobacillus are respectively expanded in liquid fermentation tank The bacterial solution obtained after culture is frozen and dried and counted, the bacterial powder is mixed according to a certain proportion, and after dilution by adding water and sugar, it is added to the fermentation bag containing crushed whole plant sweet potato, and corn cob powder is used as an auxiliary material to adjust the moisture content, and after pressure exhaust, the bio-fermentation feed is prepared after fermentation at 15-28℃ for 15-20 days.The whole plant sweet potato bio-fermentation feed prepared by the application can improve the utilization rate of sweet potato raw materials, and the vitamin content is rich, and the pork quality can be significantly improved when added to the growth-fattening pig feed.

The invention discloses a lactobacillus paracasei CCFM1529 for promoting growth and freeze-drying survival of bifidobacterium bifidum through metabolism, and belongs to the field of microbial fermentation engineering. According to the invention, the bifidobacterium bifidum is inoculated into the fermentation supernatant of the casei paracasei for culturing, so that the maximum biomass of the bifidobacterium bifidum CCFM1167 is increased by 115.17%, and the freeze-drying survival rate is increased by 20.18%.

The invention provides a bread with D-allulose instead of sucrose and a preparation method therefor, and relates to the technical field of food processing. The starting materials include, in parts by weight: 40-60 parts of flour, 3-5 parts of gluten powder, 5-10 parts of D-allulose, 5-10 parts of eggs, 5-10 parts of milk, 3-5 parts of yeast, 4-6 parts of butter, 0.4-0.6 parts of composite bacterial powder, and 15-25 parts of water. The yeast is fresh yeast. The composite bacterial powder includes one or more of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium longum and Bifidobacterium lactis. Replacing sucrose with D-allulose in bread does not affect the normal proofing of bread dough, and the baked bread is fluffy and soft, sweet, and delicious, has good color, aroma, and taste. Because D-allulose has the advantages of low calorie and low blood sugar response, consumers' pursuit of “healthy eating” is met.

The invention relates to the technical field of enzyme activity, in particular to a process for improving the activity of beta-fructosyl transferase. The invention provides a process for improving the activity of beta-fructosyl transferase. The process is characterized by comprising the following steps: activating strains, sucking lactobacillus casei, lactobacillus fermentum and bifidobacterium animalis, and respectively putting the lactobacillus casei, the lactobacillus fermentum and the bifidobacterium animalis into different culture mediums for culturing; preparing a seed solution, namely selecting bacterial colonies obtained by activating three strains, namely lactobacillus casei, lactobacillus fermentum and bifidobacterium animalis, to compound the seed solution; preparing a fermentation culture medium, namely mixing one or more of a carbon source, a nitrogen source and inorganic salt with the basic components and distilled water, fixing the volume to the use amount, and inoculating the compound seed solution. The beta-fructosyl transferase aims at solving the technical problem that in the prior art, due to insufficient activity of beta-fructosyl transferase, too much sugar is taken in a human body, and body

The invention discloses a probiotic composition, a method and application. The probiotic composition comprises 60% of lotus leaf powder, 5% of probiotics and the balance of auxiliary materials, and the proportion of the auxiliary materials is 35%. Wherein the lotus leaf powder is nanoscale lotus leaf crude extract powder, and the probiotics are freeze-dried powder and are composed of bifidobacterium bifidum and lactobacillus rhamnosus in a viable count ratio of 1: 1. The probiotic composition can be applied to any one of a granule type product, a tablet type product, a capsule type product and a granule type product. The probiotic freeze-dried powder in the probiotic composition is obtained through bacterial liquid culture and vacuum drying, and the nanoscale lotus leaf crude extract powder is obtained through reflux extraction, superfine grinding, high-temperature sterilization and the like. The invention innovatively provides a combination formula of probiotics and lotus leaf powder, and achieves the effects of effectively intervening metabolic diseases and relieving whole body chronic low-degree inflammation through an intestine-fat-immune axis regulation mechanism.

The invention belongs to the technical field of disease prognosis and molecular biology, and relates to a flora marker for colorectal cancer liver metastasis diagnosis and liver metastasis risk assessment and application of the flora marker. The marker comprises the following three strains: bifidobacterium bifidum, bacteroides fragilis and bacteroides heparinidolyticus. The method comprises the following steps: acquiring information of microorganisms in excrement of a patient through a high-resolution metagenomics detection technology; a random forest algorithm is utilized to construct a colorectal cancer liver metastasis classification model based on the flora markers, the model can distinguish people without colorectal cancer metastasis and people with liver metastasis and accurately predict high-risk patients with colorectal cancer liver metastasis, and the effect of the model is superior to that of a clinical conventional tumor serum marker. The kit has the advantages of noninvasive detection and high accuracy, and can provide a reliable tool for liver metastasis diagnosis and individualized prognosis management of colorectal cancer patients.

The present disclosure provides an integrated culture-freeze drying method for Bifidobacterium longum subsp. infantis B8762, which belongs to the field of biotechnology. The method includes: activating a strain in a carbon-source-free modified MRS culture medium and inoculating the activated strain into a fresh culture medium; adding one of D-lactose, D-sucrose or D-maltose; monitoring a growth curve, and building kinetic models in combination with metabolite analysis; collecting bacterial sludge after culture to a stationary phase, preparing a bacterial suspension, and testing a bacterial cell size, the number of growth generations, colony activity and a freeze-drying survival rate; and finally, performing vacuum freeze drying, and monitoring enzyme activity during storage. The method provided by the present disclosure can significantly improve the freeze-drying survival rate, delay attenuation of enzyme activity in a storage phase, and optimize growth and cell activity.

The invention relates to a novel lactic acid bacterium, namely, bifidobacterium bifidum P61 KCCM13367P, lactobacillus paracasei P62 KCCM13368P or a mixture of the bifidobacterium bifidum and the lactobacillus paracasei. The novel lactic acid bacteria and mixtures thereof of the present invention change the composition of intestinal microorganisms to inhibit the expression of sarcopenia markers (MuRF-1, MAFbx / Atrogin-1, etc.), increase muscle strength and muscle mass, and promote muscle formation, thereby alleviating sarcopenia. In addition, the present invention ameliorates cognitive disorders by inhibiting behaviors similar to cognitive disorders and modulating the expression of factors (BDNF, IL-10, etc.) associated with said disorders. In addition, the present invention has the effect of treating inflammation by reducing the expression of inflammatory markers (TNF-alpha, IL-6, IL-1beta, and MPO) and increasing the expression of an anti-inflammatory marker (IL-10).

A method for enhancing 5-hydroxytryptophan secretion by enterochromaffin cells is disclosed, and the method comprises: administering a composition to a subject in need thereof, the composition comprising: a Bifidobacterium animalis subsp. lactis BB-115 strain. Herein, another method for enhancing 5-hydroxytryptophan secretion by enterochromaffin cells through administering a composition to a subject in need thereof, the composition comprising: a Bifidobacterium animalis subsp. lactis BB-115 strain, a Lactobacillus rhamnosus MP108 strain, and a Bifidobacterium longum subsp. infantis BLI-02 strain.

The invention belongs to the technical field of pet oral care, and particularly relates to a composition for improving pet oral health and a preparation method and application thereof. The composition comprises the following raw materials in percentage by mass: 10%-30% of a carrier, 0.5%-5% of xanthan gum, 10%-30% of mannitol, 0.5%-5% of compound probiotics, 20%-75% of prebiotics, 0.5%-5% of spirulina, 0.5%-5% of a green tea extract and 0.5%-5% of lysozyme, and the compound probiotics comprise the following components: plant lactobacillus plantarum LP-Only, lactobacillus acidophilus LA11-Only, bifidobacterium longum subsp. Longum BL88-Only, lactobacillus mucus fermentation LF33 and lactobacillus reuteri LE16. The invention also discloses a preparation method of the compound probiotics. The composition provided by the invention can improve oral problems in multiple dimensions, and can achieve the effects of inhibiting pathogenic bacteria and eliminating peculiar smell by daily feeding.

The present invention discloses a probiotic containing Parabacteroides distasonis, which has a microorganism accession number CCTCC NO: M20222033, Limocilactobacillus fermentum, which has a microorganism accession number CCTCC NO: M2023352, Lactobacillus salivarius, which has a microorganism accession number CCTCC NO: M2023348, Enterococcus avium, which has a microorganism accession number CCTCC NO: M2023350, and Bifidobacterium bifidum, which has a microorganism accession number CCTCC NO: M2023349. The probiotic is safe, has antioxidant activity, and can inhibit pathogenic bacteria, improve colon damage, inhibit inflammation, and alleviate diarrhea caused by chemotherapy.

The application provides a kind of composite fermented inulin brewing beverage and its preparation process, belongs to brewing beverage preparation technical field;Its preparation process includes the following steps: preparation of ionic liquid;Extraction of inulin;Activation of strain;Preparation of brewing beverage.The application can reduce the interference of impurities by ionic liquid assisted polyethylene glycol and ammonium sulfate aqueous two-phase phase synergistic extraction of inulin, thereby improving purity, then improving the bioavailability of inulin by synergistic fermentation of lactobacillus salivarius AP-32 and bifidobacterium lactis Bb12, making it easier to be absorbed by human body, thereby effectively improving the effect of inulin in relieving constipation, finally, the fermented inulin is compounded with plantago seed polysaccharide to prepare a brewing beverage, thereby relieving constipation symptoms and effectively promoting defecation.

This invention discloses a respiratory health preparation of *Lactobacillus acidophilus* that enhances the mucosal barrier, relating to the field of microbial preparation technology. The preparation includes probiotic active ingredients and a pharmaceutically or food-grade acceptable carrier. The probiotic active ingredients consist of *Lactobacillus acidophilus* LH-001, *Lactobacillus rhamnosus* LR-002, and *Bifidobacterium bifidum* BB-003, which have been selectively adapted for respiratory use. It also includes synergistic functional components. By weight, the synergistic functional components include 1-5 parts of 2'-fucosylated lactose, 0.5-2 parts of low-molecular-weight sodium hyaluronate, and 0.1-0.5 parts of baicalin. The weight-average molecular weight of the low-molecular-weight sodium hyaluronate is 10-30 kDa. This invention obtains three functionally complementary exclusive strains through selective respiratory adaptation, and, combined with the synergistic effect of the synergistic components and targeted formulation technology, solves the problems of poor compatibility and difficult live bacteria delivery of conventional intestinal strains, achieving in-situ efficacy in the respiratory tract and meeting the needs of multiple commercial dosage forms.

The invention relates to the technical field of biomedical detection, in particular to a plasma circulating microbial marker for early screening of gastric cancer and application of the plasma circulating microbial marker. The plasma circulating microbial marker comprises the following components: Ralstonia solanacearum, Staphylococcus aureus, Alternaria mellea, Porphyromonas gingivalis, Lactobacillus reuteri, Rhodococcus erythropolis, Ackermania muciniphila, Lactobacillus gasseri, and Bifidobacterium bifidum, and the plasma circulating microbial marker comprises the following components: Ralstonia solanacearum, Staphylococcus aureus, Alternaria mellea, Porphyromonas gingivalis, Lactobacillus reuteri, Rhodococcus erythropolis, Ackermania muciniphila, Lactobacillus gasseri, and Bifidobacterium bifidum. The detection effect can be achieved under the condition of low depth, the screening cost is greatly reduced while the detection sensitivity is ensured, and a brand new solution is provided for early screening of gastric cancer.