126 results about "Bacterium bifidum" patented technology

The invention discloses a cosmetic with fermented original rice milk, a method for manufacturing the cosmetic and application thereof. A process for preparing the fermented original rice milk includes steps of fermenting and cultivating initial systems with bifidobacterium bifidum, dry rice flour and water to obtain the fermented original rice milk. A bacterium is the bifidobacterium bifidum. As proved by experiments, the cosmetic, the method and the application have the advantages that the fermented original rice milk does not contain optional chemical components, accordingly, the cosmetic can be directly used as a finished product of facial masks or essence or toner and is natural as compared with other products on current markets, and optional negative effects on the skins of users can be prevented; small components can be acquired by the aid of the method as compared with common extraction methods, and accordingly the cosmetic can be easily sufficiently absorbed by the skins of the users.

The invention discloses a bifidobacterium freezing concentration starter and a preparation method thereof. The preparation method can solve the problems of difficult culturing of the bifidobacterium, lower activity of the starter and short preservation period. The preparation method includes: culturing a strain activation tube, culturing the strain of first grade by a triangle bottle, culturing the strain of second grade with a seed pot, culturing in a fermentation pot, concentrically collecting the strain, adding a protective agent, packaging in vacuum and storing in a freezing way. The starter is mainly characterized by high live bacteria content, longer shelf life, less inoculation dosage, being capable of stabilizing each batch of fermented dairy products, preventing the strain from degradation and pollution as well as greatly improving the working production rate and the product quality of the fermented dairy product industry. Compared with the starter prepared by the vacuum freezing drying method, the freezing concentration starter prepared by the invention has the advantages of simple method, less device investment, lower cost and easy realizing.

The invention provides a probiotic composition and a preparation method and applications thereof, and belongs to the technical field of health-care foods. The probiotic composition comprises probiotics and prebiotics. The probiotics comprise lactobacillus acidophilus NCFM, lactobacillus rhamnosus HN001, lactic acid bacteria composite powder GI, lactic acid bacteria composite powder OH, bifidobacterium animalis B94, bifidobacterium bifidum R0071, lactobacillus casei R0215, live bacillus coagulans GBI30,6086 and lactobacillus rhamnosus GG. The prebiotics include fructo-oligosaccharides, resistant dextrins and lactitol. The mass ratio of the probiotics to the prebiotics is (300-350):(1300-1400). The total viable bacteria concentration of the probiotics in the probiotics composition is more than or equal to 9.0x10<9> CFU / g. The probiotic composition provided by the invention can obviously improve sleep quality, and significantly relieve anxiety and depression.

The invention discloses an active lactic acid bacteria beverage and a preparation method thereof. According to the invention, optimum inoculation proportion of lactobacillus acidophilus to bifidobacteria is 1-100: 1, compared with a traditional bacterial classification-fermented active lactic acid bacteria beverage, the active lactic acid bacteria beverage in the invention has stronger probiotic role, and the active lactic acid bacteria beverage has good local flavor and fills with blank of the pure probiotics fermented active lactic acid bacteria beverage on market. In the invention, excessive oxygen in a fermentation bed material during a fermentation process can be consumed by adding an anti-oxidant before fermentation, anaerobic type lactobacillus acidophilus and bifidobacteria enable good growth, and fermentation time can be shortened. The method is suitable for preparing the active lactic acid bacteria beverage.

Use of at least one strain of a microorganism and / or a metabolite thereof in the manufacture of a support for administration to a subject for modulating satiety signalling, wherein the support is a pharmaceutically acceptable support or a food product. Suitably, the at least one strain of a microorganism and / or a metabolite thereof may be administered to the subject for the treatment and / or prevention of excess weight and / or a disease caused by excess weight. Likewise, the at least one strain of a microorganism and / or a metabolite thereof is administered to the subject for the treatment and / or prevention of obesity and / or a caused by obesity. Preferably, the microorganism is a probiotic microorganism. Suitably the microorganism may be a lactic acid bacterium. Li one embodiment the microorganism is a strain of Lactobacillus spp. and / or Bifidobacterium spp., for example a strain of Lactobacillus acidophilus, L. curvatus, L. salivarius and / or B. lactis.

The invention discloses fermentation bed padding for ecologically breeding sheep. The fermentation bed padding has the advantages of rapid growth promotion, strong immune effect, rapid and thorough excrement degradation, rich raw material source, simple production process, low cost and the like, the production performance and the body immunity of the sheep can be remarkably improved, diseases are reduced, the economic benefit for sheep breeding is improved, and clean production, ecological cycle and healthy breeding are achieved. Excrement, bran, alfalfa and straws are used as raw materials, saw dust, wormcast, distilled grain, Chinese herb mixtures and the like are used as auxiliary materials, and the fermentation bed padding is obtained through a three-step fermentation process of a biological fermentation agent; the fermentation bed padding is rich in probiotics such as yeast, aspergillus niger, bacillus subtilis, bacillus licheniformis, lactobacillus and bifidobacterium bifidum and Chinese herb immune active components; after the fermentation bed padding is applied to sheep breeding, the morbidity of the sheep is low, the growth of the sheep is promoted favorably, and the fermentation bed padding has a greater application and popularization prospect.

The invention discloses ginseng fermentation puree as well as a preparation method and an application thereof. The preparation method of the ginseng fermentation puree comprises the steps that dry ginseng powder, water and bifidobacterium bifidum are mixed to obtain an initial system, the initial system is subjected to fermentation cultivation, and ginseng fermentation puree is obtained. The ginseng fermentation puree doesn't contain any chemical component, foreign matters such as enzyme are not required to be added, not only is production cost saved, but also the production steps are simplified maximally, the fermentation technology can realize mass production and industrial production, and the product quality stability can be fully guaranteed. At the same time, the ginseng fermentation puree can be directly used as a finished production of a facial mask, essence or toner and is more natural compared with other products existing in the market, and no negative effect is caused to the skin; compared with ordinary extraction method, low-molecular-weight peptide can be obtained, the skin can absorb the ginseng fermentation puree thoroughly, and the ginseng fermentation puree can be widely applied in preparing anti-aging products and / or DPPH free radical clearing products.

The present invention discloses a production technology of a rosa roxbunghii enzyme and belongs to the technical field of enzyme preparation. The rosa roxbunghii enzyme is prepared from fresh and non-rotten rosa roxbunghii as a raw material, lactic acid bacteria, lactobacillus acidophilus, bifidobacterium longum, yeasts, etc. are used as fermentable powder to conduct a fermentation, a certain amount of isomaltooligosaccharides and probiotics are added, the probiotics use the isomaltooligosaccharides as nutrition, the rapid fermentation is conducted to form the enzyme, and when the enzyme reaches a certain concentration, enzyme enriched raw liquid is obtained.

The invention relates to a grape probiotic fermentation beverage. Squeeze juice of grape is used as a culture medium; fermentation is carried out under specific temperature and for specific time to culture probiotics, such as bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus, lactic acid bacillus and the like; then, probiotic liquid after fermentation is inactivated, and then aproper amount of white granulated sugar is added to make a beverage. Slag byproducts generated in the production of the grape probiotic fermentation beverage can be also used for producing grape probiotic jam. The invention greatly broadens the application range of probiotic culture medium selection of materials, such as bifidobacterium and the like, provides a novel probiotic inactivation fermentation beverage, and has the advantages of rich material source, low cost, stable preparation process, little production equipment investment, simple operation, easy popularization and favorable market application prospect.

The invention discloses a process for preparing a fermentation mattress material through resource utilization of sheep manure. The process is high in resource utilization degree, facilitates large-scale application, helps to realize harmlessness processing of sheep culture wastes and resource utilization of the fermentation mattress material and solves the two major problems of sheep culture environmental pollution and resource shortage of the fermentation mattress material. According to the process, the sheep manure is taken as a raw material, with assistance of straws, earthworm manure, vinasse, Chinese herbal medicine mixtures and the like, the fermentation mattress material is prepared through the three-step fermentation process of a biological starter, the fermentation mattress material is rich in such probiotics as microzyme, aspergillus niger, bacillus subtilis, Bacillus licheniformis, lactic, Bifidobacterium bifidum and the like, the content is as high as 2-5*10<8>cfu / g, after the process provided by the invention is used for pig culture, the disease incidence of pigs is reduced, growth of the pigs is facilitated, and the application and popularization prospect is quite good.

The invention relates to a compound metagen solid beverage and a preparation method thereof. The solid beverage is prepared from the following components in percentage by mass: 15-20% of sulforaphane, 20-25% of sodium butyrate, 25-30% of metagen solid powder, 18-22% of an adhesive, 4.5-6.0% of a sweetening agent and 3.5-6.0% of a sour agent, wherein the metagen solid powder comprises lactobacillus paracasei G15 and Q14 powder, lactobacillus rhamnosus powder and bifidobacterium powder; and the four kinds of lactobacillus powder are obtained by drying supernatants of respective bacilli. The metagen solid beverage is especially suitable for crowds with special requirements, such as crowds with hyperlipidemia, crowds with fatty liver and crowds with weight loss.

The invention discloses a detoxifying agent for cottonseed meal and rapeseed meal as well as a preparation method thereof and a preparation method of a microorganism feed prepared by the detoxifying agent, wherein the detoxifying agent is prepared from the following raw materials: mixed bacterial liquid, oligofructose, glucose, edible salt and softened water; the mixed bacterial liquid is prepared from the mixture of soluble phosphine bacteria, bifidobacterium and lactobacillus acidophilus. The preparation method comprises the following steps of mixing, fermentation, temperature reduction andfermentation again, thus the finished product can be packaged. Because the technical scheme is adopted, the detoxifying agent provided by the invention has the detoxifying effect of 76-97% on a rapeseed pancake, and removes most anti-nutritional factors; the products after detoxification have good adaptability to animals and have special flavor, the product protein after fermentation and detoxification can be improved by 8-12%, meanwhile vitamin and digestive enzyme are also rich, and the amino acid utilization ratio is improved by 11.9%. According to the invention, the process is simple, theflow is short, the investment is less, and environment pollution is not generated.

The invention discloses an external probiotics microcapsule preparation method. By making full use of the synergistic effect of plant extracts and probiotics, on one hand, survival rate and entrapment rate are cooperatively increased; on the other hand, the plant extracts have a further effect on skin care. The external probiotics microcapsule preparation method includes the steps: (1) respectively inoculating freeze-dried powder of bifidobacteria, lactobacillus acidophilus and lactobacillus rhamnosus into a triangular flask for activating treatment, enlarging fermentation cultivation and mixing fermentation liquor of the bifidobacteria, the lactobacillus acidophilus and the lactobacillus rhamnosus according to the volume ratio 3:3:4 to obtain bacterial suspension; (2) preparing nostoc commune extracts; (3) adding Tween-80 for emulsion into the bacterial suspension, the nostoc commune extracts and sodium alginate solution, curing the bacterial suspension, the nostoc commune extracts and the sodium alginate solution, filtering the bacterial suspension, the nostoc commune extracts and the sodium alginate solution by gauze after a wet capsule is formed, and flushing the wet capsule for three times by normal saline.

The invention discloses a preparation method of freeze-dried Bifidobacterium lactis powder. A basic medium adopted in the invention comprises yeast peptones, yeast extract, glucose, fructo-oligosaccharide, L-malic acid and purified water, and has a pH value of 7.2; and an optimized medium includes yeast peptones, yeast extract, glucose, fructo-oligosaccharide, L-malic acid, lactose, calcium chloride and purified water, and has a pH value of 7.2, and the number of live bacteria in a fermentation broth can reach 5.53 * 10<9> cfu / ml or more. A freeze-dried bacterium powder protection agent comprises trehalose, skim milk powder, sucrose, ascorbic acid, glucose and purified water, and the total number of viable bacteria in the free-dried Bifidobacterium lactis powder can reach 9.0 * 10<11> cfu / g or more. The preparation method can effectively achieve the purposes of high-density culture of Bifidobacterium lactis, a high bacterium yield and a large number of variable bacteria in the freeze-dried powder, and facilitates realization of large-scale industrial production.

The invention belongs to the field of preparation of enzymes and application of the enzymes in cosmetics, and particularly relates to an European bilberry enzyme prepared from composite biological enzymes and a preparation method and application thereof. After bilberries are subjected to enzymolysis, sugar is added, and sealed fermentation is carried out with mixed bacteria to obtain the bilberryenzyme. The mixed bacteria comprise Saccharomyces cerevisiae, Lactobacillus plantarum, and Bifidobacterium. The invention explores a new process of bilberry enzyme fermentation, the process is simple,nutrient substances and effective components in the bilberries are fully released, soluble substances are completely dissolved, and the bilberry enzyme is suitable for cosmetic addition. The cosmetics can be conventional preparations such as masks, toning lotions or emulsions. After the bilberry enzyme is added into essence, the skin care effect is obviously enhanced.

The invention discloses a preparation method of a pharmaceutical composition for treating acnes. The method is characterized by comprising a step of fermenting traditional Chinese medicinal materials with bifidobacterium, lactobacillus acidophilus and lactobacillus plantarum. Cosmetics are prepared by a conventional method in pharmaceutics. An experiment proves that the curative effect can be further improved.

The invention provides a chewable egg powder tablet and its preparation method, and belongs to the technical filed of egg product processing. The chewable egg powder tablet is prepared by adopting specific raw materials and auxiliary materials according to a specific ratio. Lactobacillus bulgaricus, Streptococcus thermophilus, Bifidobacterium bifidum and Lactobacillus casei are used together according to a ratio of 4:2:1:2, and fermentation is carried out in under unique multistep reaction conditions, so natural, pure and nice flavor and abundant nutrition are realized. The chewable egg powder tablet has balanced nutrition, and is endowed with high nutritional values through the synergistic effect of the above components. The preparation method has the advantages of simple operation technology, and easy realization of the industrialized production, and provides a new approach for the advanced processing of eggs.

An isolated lactic acid bacterium strain or fermentation metabolite thereof has an anti-oxidant function and is in form of a food composition or a pharmaceutical composition, wherein the isolated lactic acid bacterium strain comprises at least one of an OLP-01 strain of Bifidobacterium longum subsp. longum; a Bv-889 strain of Bifidobacterium breve; a BLI-02 strain of Bifidobacterium longum subsp. infantis; a CP-9 strain of Bifidobacterium animalis subsp. lactis; a Bf-688 strain of Bifidobacterium bifidum; a GL-104 strain of Lactobacillus reuteri; an AP-32 strain of Lactobacillus salivarius subsp. salicinius; and a bv-77 strain of Lactobacillus rhamnosus.

The invention discloses a solid beverage with lipid-lowering and antioxidant effects and a preparation method of the solid beverage, which relates to the field of nutritional health food, the solid beverage disclosed by the invention comprises the following raw materials in parts by weight: 20 to 50 parts of polydextrose, 10-30 parts of fructo-oligosaccharide, 5-15 parts of L-arabinose , 5-15 parts of inulin, 5-15 parts of lactose, 5-10 parts of maltodextrin, 2-10 parts of corn starch, 0.1-5 parts of lactobacillus plantarum, 0.1-5 parts of lactobacillus rhamnosus, 0.1-5 parts of bifidobacterium longum, 0.1-5 parts of bifidobacterium bifidum, 0.1-5 parts of bifidobacterium lactis and 0.1-5 parts of lactobacillus reuteri. The collagen peptide, the probiotics, the prebiotics and other raw materials are functionally fused, the raw materials are natural, scientific in formula, balanced in nutrition and unique in flavor, and all the raw materials have a synergistic effect, so that the beverage has a beautifying function and also has an effect of promoting defecation; meanwhile, the collagen peptide and other auxiliary materials except the bacteria powder are fused by adopting a granulation process and finally mixed with the probiotics, so that the health-care food has the advantages of being good in taste and convenient to eat.

The present invention relates to a composition for producing a fermented milk product showing a low degree of post-acidification during storage comprising ⋅ 1) a starter culture of lactic acid bacteria comprising at least one lactose-deficient Streptococcus thermophilus strain, which is capable of metabolizing a non-lactose carbohydrate, and at least one lactose-deficient Lactobacillus delbrueckii subsp. bulgaricus strain, which is capable of metabolizing the non-lactose carbohydrate, and ⋅ 2) a probiotic strain selected from the group consisting of Lactobacillus strain and a Bifidobacterium strain.

The invention discloses a microbial preparation for keeping fruits and vegetables fresh and a preparing method thereof. The microbial preparation is prepared from, by weight, 50-70 parts of bifidobacterium, 50-70 parts of lactobacillus acidophilus, 0.5-1 part of spermidine, 700-1000 parts of molasses, 200-300 parts of saccharose and 2000-3000 parts of ultrapure water. The method specifically comprises the steps of inoculating an ultrapure water nutrient solution containing molasses and saccharose with bifidobacterium and lactobacillus acidophilus for airtight constant-temperature fermentation at the temperature of 28-33 DEG C and the pH value of 6-7 for 7-9 days; centrifuging a liquid product generated after fermentation at the rate of 6000 r / min, and adding spermidine to supernate for sufficient mixing, so that a liquid mixture, namely a preservative stock solution is obtained; diluting the preservative stock solution by 200-400 times to obtain a preservative working solution. By the adoption of lactic acid bacteria, organic acid such as lactic acid, acetic acid and propionic acid can be generated during production, the pH value of the environment can be reduced, and growth of harmful bacteria in the environment is inhibited. Peptides active substance like bacteriocin can be generated by lactic acid bacteria, so that growth of gram-positive bacteria which can promote food spoilage is inhibited.

A fermentation metabolite generated by an isolated lactic acid bacterium including at least one of a Bv-889 strain of Bifidobacterium breve, a BLI-02 strain of Bifidobacterium longum subsp. infantis, a CP-9 strain of Bifidobacterium animalis subsp. lactis, a Bf-688 strain of Bifidobacterium bifidum, an AP-32 strain of Lactobacillus salivarius subsp. salicinius, and a GL-156 strain of Lactobacillus paracasei. The fermentation metabolite of the abovementioned strains have a skin-whitening effect and is in form of a food composition or a cosmeceutical composition.

The invention relates to the field of fermentation engineering, in particular to a ganoderma lucidum fruiting body composite probiotic preparation for enhancing organism immunity. A fermentation product is obtained by co-fermentation of a ganoderma lucidum fruiting body extracting solution and a composite probiotics strain, wherein the composite probiotics comprise lactobacillus acidophilus and bifidobacterium breve in a number ratio of viable bacteria of 1 to 1; the ganoderma lucidum fruiting body powder is prepared into the ganoderma lucidum extracting solution with the concentration of 8.8-33.2mg / ml, and then the extracting solution is added into an MRS culture medium with 0.5% of sugar. Next, the culture medium is inoculated with the lactobacillus acidophilus and bifidobacterium breveat the concentration of 1% separately, and culturing is carried out at 37 DEG C for 48h for fermentation to obtain the fermentation product. The preparation can relieve the phenomenon of low immunityand enhance the immunity of the organism. Meanwhile, the problem of low utilization rate of the precious Chinese herbal medicine which refers to the ganoderma lucidum can be relieved.

The invention discloses a health food capable of adjusting the functions of an intestinal flora and a preparation method of the health food. The preparation method comprises the following steps of 1) taking Lactobacillus acidophilus bacterial powder, Bifidobacterium bifidum bacterial powder, bifidobacterium longum bacterial powder and fructo-oligosaccharide, and sieving separately for later use; and 2) weighing Lactobacillus acidophilus bacterial powder, Bifidobacterium bifidum bacterial powder, bifidobacterium longum bacterial powder, maltodextrin and apple fruit powder according to a formula ratio, adding into a mixing machine for mixing, controlling the rotating speed to 120 to 150 rpm, then mixing with fructo-oligosaccharide in a total mixing machine, controlling the rotating speed to 120 to 150 rpm until the color and luster are consistent without color difference, subpackaging to obtain the health food, wherein the health food comprises the raw materials and the auxiliary materials according to the formula ratio in the step 2) : 40 to 60 parts of Lactobacillus acidophilus bacterial powder, 40 to 60 parts of Bifidobacterium bifidum bacterial powder, 40 to 60 parts of bifidobacterium longum bacterial powder, 1200 to 1600 parts of fructo-oligosaccharide, 200 to 300 parts of maltodextrin and 200 to 300 parts of apple fruit powder.

The invention discloses multiple-fluorescence quantitative PCR primers, a kit and detection method for detection of multiple types of bacteria. The primers include 16SrDNA designed primer pairs and probes which target Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium animalis, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus reuteri and Akkemansia, wherein the nucleotide sequences of the primer pairs of Bifidobacterium longum, animalis Bifidobacterium and Bifidobacterium breve are shown in SEQ ID NO:1 and SEQ ID NO:2. The invention also discloses a multiple-fluorescence quantitative PCR detection kit which comprises the PCR primers and probes. The multiple-fluorescent quantitative PCR primers and probes for detection of multiple typesof bacteria are specific-sequence primers and probes which are designed and synthesized according to multiple experiments, and through the optimized specific primers and probes, it is ensured that good specificity and reduction of mutual interference among primer probes in the same reaction system can be achieved during amplification.

The present invention discloses a probiotics sea buckthorn fruit puree jelly and a preparation method thereof, and belongs to the field of probiotics. A sea buckthorn fruit puree jelly is used as a carrier of probiotics; proteins, polysaccharides and other ingredients in sea buckthorn fruit puree are utilized; and at the same time, a gel substance is combined with thalli of bifidobacteria, lactobacillus, etc. to form the probiotics sea buckthorn fruit puree jelly. The probiotics sea buckthorn fruit puree jelly effectively reduces heat, oxidation and drying damages to the probiotics during a conventional drying process, achieves a purpose of long-term preservation of the probiotics, at the same time plays a role of resisting oxidation damages, effectively prevents vigor loss of bacterial strains during a storage process, also improves the additional nutritional value of the probiotics, and is widely used in food industry.

The invention provides a soilless potting matrix, which is mainly prepared by the following components in parts by weight: 10 parts of extracted residue of traditional Chinese medicine, 60-80 parts of waste fruits and vegetables, 4-8 parts of bifidobacterium, 2-8 parts of lactobacillus, 2-6 parts of aspergillus flavus, 30-50 parts of perlite, 40-60 parts of peat and 0.2-0.8 part of ammonium chloride. The invention also discloses a preparation method of the soilless potting matrix. Compared with the prior art, the soilless potting matrix prepared by the preparation method provided by the invention is low in cost, simple in technology, can completely replace soil and meet nutritional requirement of potted plants, and also can overcome the defect caused by directly usage of the extracted residue of traditional Chinese medicine and avoid plant diseases and insect pests.

A preparation method of a traditional Chinese medicine microecological feed additive for layers at early period of laying comprises the steps of taking traditional Chinese medicines, i.e., herba dandelion, common andrographis herb, radix isatidis, herba motherwort, herba purple common perilla, radix scutellariae, eucommia ulmoides leaves, astragalus root, pilose asiabell root and licorice root, performing superfine grinding on the traditional Chinese medicines and adding ethanol solution to boil through high heat, preserving temperature with gentle heat, cooling, filtering, concentrating filter liquor, adding white sugar to boil, cooling to room temperature, then inoculating lactobacillus, tea fungus and bifidobacterium, fermenting until a white fungus block grows, and concentrating bacteria solution at low temperature; then weighing modified starch, Arabic gum, cyclodextrin and syrup, adding into the concentrated bacteria solution, stirring while adding, homogenizing at high temperature after mixing and stirring uniformly, and performing spray drying to obtain the traditional Chinese medicine microecological feed additive. The traditional Chinese medicine microecological feed additive has the efficacies of increasing beneficial bacteria in intestine, improving immunity, stress resistance and disease resistance of chicken body, and increasing egg yield. Compared with the control group, the laying rate is increased by 4.68% and 5.72%, the egg-feed ratio is decreased by 5.28% and 7.56%, a rate of eggs with soft shells and shell cracks is decreased by 32.17% and 22.46%, the feed is saved, costs are reduced, and economic benefits are increased.

The invention discloses a production technology of wild kiwi fruit ferment, and belongs to the technical field of preparation of ferment. According to the wild kiwi fruit ferment, fresh wild kiwi fruits which do not become putrid are used as a raw material, lactic acid bacteria, lactobacillus acidophilus, bifidobacterium longum, microzymes and the like are used for ferment fermentation, and a certain quantity of isomaltooligosacharide and probiotics are added. The probiotics rapidly ferment to form the ferment based on nutrient namely the isomaltooligosacharide, and when the ferment achieves acertain concentration, a stock solution rich in the ferment can be obtained.