The invention relates to a preparation method and an application of
naked eye identification and
fluorescence enhancement type mercaptoamino-acid
fluorescence probes, and specifically relates to preparation based on
boron difluoride-dipyrromethane derivatives and an application thereof in detecting mercaptoamino-acids. The
fluorescence probes are synthesized through
direct reaction of the
boron difluoride-dipyrromethane derivatives with
nitromethane, and the pure probe product can be obtained through
column chromatography analysis. The fluorescence probes are simple and convenient to synthesize, mild in
reaction conditions, easy to purify and high in synthesis yield. The maximum absorption
wavelength of the probe molecules is at 519nm; the probe molecules are good in
solubility in a 50%
acetonitrile aqueous solution and stable in optical properties; the absorption
wavelength of the probe molecules is transferred to 506nm from 519nm as the mercaptoamino-acids are added; the fluorescence spectrum of the probe molecules is continuously enhanced at 520nm, and enhanced by 150 times at most, and in the meantime, the corresponding solution color turns to yellow from pink. The
naked eye identification and fluorescence enhancement type mercaptoamino-acid fluorescence probes overcome the shortcoming that the response of the existing fluorescence probes to the mercaptoamino-acids is only based on the change of the
fluorescence intensity. The probe molecules are high in sensitivity, high in identification ability to the mercaptoamino-acids and high in response speed, the response range of the probe molecules is from 0 to 1000 micrometers, the detection limits of the probe molecules to Cys, CSH and Hcy are 0.45
micrometer, 0.21
micrometer and 0.12
micrometer, and therefore, the detection range is wide and the detection limits are low; in short, the probes of the type have important practical application value in the fields such as
biochemistry and environmental science.