The invention discloses a winter jujube dedicated biological organic fertilizer, manure and seaweed are taken as fermentation substrates and compounded with trace elements, and the fertilizer integrates efficacies of seaweed fertilizers, microbial fertilizers and trace element fertilizers into a whole; with scientific compatibility application of cyrtomium rhizome, fructus toosendan, polygonum aviculare, radix scutellariae, rhizoma imperatae, spica prunellae, herba lysimachiae, radix achyranthis bidentatae, loofah sponge, fructus cnidii, eclipta prostrata, artemisia apiacea, motherwort and virgate wormwood, and through a liquid fermentation and solid fermentation combined technology, bacillus subtilis, aspergillus niger, effective microorganisms, photosynthetic bacteria, lactobacillus casei and other probiotics are added into soil with high content and high activity, active ingredients promoting plant metabolism, resisting bacteria and suppressing diseases are extracted from Chinese herbal medicines, and thus the fertilizer has the effects of raising flowers, improving the fruit setting rate, killing insects, preventing the diseases, degrading pesticide residues, saving expenditure, increasing production and improving the quality of winter jujube, also has double efficacies of preventing the diseases and increasing fertility, is beneficial for improving the soil environmental pollution, and has wide application prospects.