The invention discloses a mixing device for preparing an atriplex canescens cultivation substrate and the cultivation substrate, the device combines powder spraying, rolling and stirring, the stirringefficiency is improved, the mixing sufficiency is facilitated, and the uneven mixing caused by directly adding a small amount of powder into a large amount of basic substances is avoided. The cultivation substrate is prepared from the following raw materials in parts by weight: 30-90 parts of a humic acid mixture, 30-70 parts of peat, 40-80 parts of sand, 10-40 parts of vermiculite, 0.8-2.0 partsof chitin, 5-10 parts of polyacrylamide, 0.5-1.0 part of Pujuxitang, 0.1-0.5 part of a dispersing agent, 0.2-0.4 part of a wetting agent, 0.1-0.5 part of white carbon black and 1-3 parts of inorganiccarrier. The culture medium provided by the invention is mainly prepared from peat, sand, vermiculite and the like, is good in air permeability, strong in water retention capacity and rich in nutritional ingredients, and is beneficial to enhancing the autoimmunity of the atriplex canescens, reducing the infection of bacteria on the atriplex canescens, improving the survival rate of the atriplex canescens and promoting the growth of seedlings by combining the characteristics of nano chitin, methanesulphonamide and Pujuxitang. And a good foundation is laid for cutting seedling of the atriplex canescens.