The invention discloses a mildew-resistant water-based paint for an interior wall and a preparation method thereof. The mildew-resistant water-based paint for the interior wall comprises 25-35 parts by weight of an acrylic emulsion complexed with silver ions, 5.0-7.5 parts by weight of an opaque polymer, 16-18 parts by weight of titanium dioxide, 3-5 parts by weight of diatomite, 7.5-10 parts by weight of ground calcium carbonate, 0.8-1 part by weight of a freezing-and-thawing-resistant stabilizer, 1-2 parts by weight of a film-forming aid, 0.5-0.8 part by weight of a dispersing agent, 0.3-0.5part by weight of a thickener, 0.1-0.2 part by weight of a wetting agent, 0.1-0.3 parts by weight of an antifoaming agent, 0.1-0.3 part by weight of a preservative, 0.5-0.8 part by weight of an anti-mold agent, 0.2-0.3 part weight of a leveling agent and 20-30 parts by weight of water. The mildew-resistant water-based paint of the invention has the advantages of friendliness to environment, highcovering power, strong adhesion, good leveling properties, excellent and long-lasting mildew resistance, good comprehensive performance, high cost performance, strong applicability, low gloss at a measurement angle of 85 degrees, etc., and can be widely used in food processing plants, pharmaceutical plants, underground garages, grain depots, breweries and other places.