The invention belongs to the field of carotenoids, and particularly relates to a new synthesis method of epsilon-carotene. The method comprises the following steps: dissolving 2,7-dimethyl-2,4,6-octanetriene-1,8-bisphosphonate in an anhydrous solvent, adding an alkali solution under the protection of nitrogen, and performing stirring to obtain a reaction solution 1; dropwise adding 3-methyl-5-(2,6,6-trimethyl-2-cyclohexane-1-yl)-2,4-pentadienal dissolved in the anhydrous solvent to the reaction solution 1, dropwise adding deionized water after the above dropwise addition reaction is completed,and performing uniform stirring to obtain a reaction solution 2; performing reduced pressure distillation on the reaction solution 2 to remove the organic solvent in order to obtain an aqueous solution, performing extraction by using the organic solvent, drying the obtained extract by anhydrous magnesium sulfate, and filtering the dried extract to obtain an organic solution; and distilling off the organic solvent in the organic solution, adding an alcohol solvent, performing a refluxing reaction under the protection of nitrogen, and cooling and filtering the obtained reaction solution to obtain the epsilon-carotene. The preparation method has the characteristics of simple process route, convenience in operation, high yield, low cost, recyclable byproducts and good industrialization values.