The invention relates to a high-temperature acidification corrosion inhibitor and a preparation method thereof. The high-temperature acidification corrosion inhibitor is prepared from the following components in parts by weight: 1-5 parts of N-alkylamino-2-perfluoroalkyl imidazoline quaternary ammonium salt, 30-50 parts of hydrocarbon imidazoline quaternary ammonium salt, 0-5 parts of sodium molybdate, 5-15 parts of thiocarbamide, 20-30 parts of ethanol and 10-20 parts of water. The preparation method comprises the following steps: adding the raw materials into a reaction kettle in turn, and stirring at 20-80 DEG C for 10-30 minutes to obtain the high-temperature acidification corrosion inhibitor. As the high-temperature acidification corrosion inhibitor contains the N-alkylamino-2-perfluoroalkyl imidazoline quaternary ammonium salt with special properties, compared with the conventional acidification corrosion inhibitor, the high-temperature acidification corrosion inhibitor provided by the invention has the advantages of low addition amount, good water solubility, strong temperature resistance, high corrosion inhibition rate and the like. When being used under acidification conditions at 150-200 DEG C, the high-temperature acidification corrosion inhibitor still has good corrosion inhibition effect and compatibility. The invention has the advantages of high corrosion-inhibition efficiency, low toxicity, environmental protection, high temperature resistance, wide application systems and the like, and can be used as the component of a high-temperature acidification liquid corrosion inhibitor.