The invention relates to a traditional Chinese medicine biological preparation for promoting female reproductive health and a preparation method thereof. The traditional Chinese medicine biological preparation comprises radix sophorae flavescentis, fructus cnidii, gallnut, aloe, radix angelica sinensis, cortex phellodendri, safflower, semen cuscutae, roselle, cranberry, propolis, lysozyme, mentholand soybean peptide. The traditional Chinese medicine biological preparation has the advantages that the traditional Chinese medicine biological preparation adopts pure natural traditional Chinese medicine components, is free of irritation to the vagina mucosa, does not destroy the clean environment of the vagina, removes the vagina peculiar smell, eliminates itching, inflammation and erosion, nourishes the vagina, has the functions of bacteriostasis, bacterium resistance, virus resistance and the like, can inhibit the growth and reproduction of bacteria, can be used for repairing the vaginalmucosa and the cervical injury, and also can promote elasticity and tightness restoration of the vagina, protect the ovary, improve the immunity of organisms and promote the female reproductive health.