The invention discloses golden orange, almond and lily rice vinegar and a preparation method thereof. The golden orange, almond and lily rice vinegar is prepared by the following raw materials in part by weight: 500-550 parts of golden oranges, 100-120 parts of cherry juice, 20-25 parts of rice vinegar, 50-55 parts of honey, 30-35 parts of almond, 15-20 parts of cuttlefish ink, 10-15 parts of schizonepeta flower, 30-35 parts of pigskin collagen, 10-12 parts of aloe extracts, 5-8 parts of mint leaves, 30-35 parts of pickled peppers yam, 5-10 parts of water chestnut leaves, 25-30 parts of lily, 15-20 parts of nutrition adding liquid and appropriate deionized water. Effective ingredients of the golden orange, almond and lily rice vinegar have effects of allaying tiredness, improving human immunity, protecting skin, clearing away the lung-heat, moistening dryness, relieving cough, resisting bacteria and diminishing inflammation. The golden orange, almond and lily rice vinegar tastes sour and sweet, is clear and bright in color, can be orally taken in daily life and can also be used during cooking.