The invention discloses novel brewing yeast and a making method thereof. The brewing yeast is prepared from oat, euscaphis japonica, Chinese ephedra, rhizoma atractylodis macrocephalae, bletilla striata, amomum cardamomum, soaked ginseng, cockscomb, nux vomica, radix angelicae pubescentis, mangnolia officinalis, schizonepeta, notopterygium roots, uncaria, safflower carthamus, immature bitter oranges, rhizoma zingiberis, perilla leaves, pepper, Indian trumetflower seeds, croton, radix aconiti carmichaeli, radix aconiti, galangal, salvia miltiorrhiza, pillis ophidiae, angelica sinensis, asarum sieboldii, radix aconiti agrestis, cinnamon, zaocys dhumnade, cassia twigs, rhizoma atractylodis, elecampane, cortex acanthopanacis, cortex lycii radicis, apiary, codonopsis pilosula, momordica cochinchinensis, herba epimedii, periostracum cicada, pericarpium citri reticulatae, fructus amomi, centipede and white-dappled snakes. The making method includes the steps of oat crushing, medical material crushing, yeast material stirring and fermentation for making yeast. Compared with conventional brewing yeast, the novel brewing yeast is high in fermentation capacity, long in shelf life, simple in making method and rich and complete in nutrition, and the baijiu brewed through the brewing yeast is mellow in taste, heavy in fragrance, good in mouthfeel, free of bitter and astringent flavor, stable in quality and capable of being stored for a long time and has a good health care effect.