50 results about "Peribacteroid membrane" patented technology

The invention provides a sunlight-excited multi-ROS (reactive oxygen species) response oxygen sensitization type hydrogel. The sunlight-excited multi-ROS response oxygen sensitization type hydrogel is prepared from a plant-derived nano capsule-like body (NT), an AQPP liposome (AL) and pluronic F127 serving as a hydrogel matrix. The invention also provides a preparation method of the hydrogel and application of the hydrogel in preparation of medicines for treating skin ulcers. The NTALG causes bacterial membrane function damage after illumination, has a strong antibacterial effect and can promote healing of diabetic wounds.

The present invention belongs to the field of microbiology, and particularly relates to membrane vesicles (MVs) isolated from bacteria, and an isolation and preparation system and method for the membrane vesicles, and applications of the membrane vesicles. The bacteria of the present invention comprise Gram-positive bacteria and Gram-negative bacteria. The invention uses ionizing irradiation to irradiate bacteria, and isolates and purifies the produced membrane vesicles. The membrane vesicles prepared can be used as a vaccine, a vaccine adjuvant and / or a pharmaceutical carrier. In addition, the present invention provides a biological composition comprising the membrane vesicles and inactivated bacteria. In addition, the present invention also provides a preparation system, and isolation and purification system for bacterial membrane vesicles and the corresponding method. The membrane vesicles obtained by using the system and method have high yield, high purity and easy to be industrialized.

The present application provides a bionic antibacterial peptide, a pharmaceutical composition and its application in the preparation of antibacterial drugs, as well as a design method of the bionic antibacterial peptide. A variety of compounds are used to modify the N-terminus of a polypeptide sequence composed of 20 natural amino acids, thereby generating a novel antibacterial peptide with bionic functions. Moreover, a method based on the interaction between deep learning and experiments is adopted to screen the designed bionic antibacterial peptide. The time, manpower, material resources and financial resources consumed are greatly reduced compared with traditional drug screening. In addition, the designed novel bionic antibacterial peptide has good biocompatibility. The functional groups modified at the N-terminus can activate the self-assembly activity of the polypeptide and help the polypeptide target the bacterial membrane, which is not easy to cause bacterial drug resistance, can effectively kill drug-resistant bacteria, has a faster bactericidal speed, and has potential and broad application prospects in the treatment of clinical bacterial and drug-resistant bacterial infectious diseases.

The invention discloses a fatty cation amphiphilic peptide simulant as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, developing an amphipathic cationic peptide simulant system based on cysteine, then introducing ethylenediamine as a cationic group by forming a peptide bond at the terminal of carboxyl, and introducing a hydrophobic group containing an aromatic ring to a sulfydryl side chain; wherein the cationic group provides positive charges and hydrophilicity and is beneficial to electrostatic interaction with a bacterial membrane with negative charges, and the hydrophobic group is beneficial to insertion of the membrane into a phospholipid bilayer; in addition, the N terminal is modified by fatty acids with different chain lengths, the amphiphilic balance of the cysteine-based cationic peptide simulant is finely adjusted, and finally the fatty cationic amphiphilic peptide simulant is synthesized. The peptidomimetic has good antibacterial activity, is not easy to induce bacteria to generate drug resistance, and can be used for preparing drugs for treating, improving and / or preventing infection of drug-resistant bacteria and / or drug-resistant fungi.

The invention belongs to the technical field of high polymer materials, and particularly discloses a medical antibacterial film material and a preparation method thereof.The antibacterial film material has an excellent antibacterial effect, by adding modified polyvinyl alcohol and modified graphene, the completeness of cell membranes of bacteria can be effectively destroyed, and the antibacterial effect is good. The antibacterial agent has the advantages that the antibacterial effect is obviously improved under the synergistic interaction of the two components, the metabolic process of bacteria is interfered, the growth of the bacteria is inhibited, and the bacterial membrane is destroyed by electrostatic adsorption, so that the problems of easy drug resistance and compatibility in the system of a single antibacterial component are solved.

This invention relates to a novel, highly efficient bactericide and its preparation method, belonging to the field of antibacterial materials technology. The invention utilizes the reaction of epoxy groups and tertiary amine compounds in maleic pine carboxylic acid glyceride under hydrochloric acid to convert the epoxy groups into a hydroxyl quaternary ammonium salt structure. Then, an esterification reaction between trimellitic anhydride acyl chloride and hydroxyl groups is used to introduce an anhydride group into the molecular chain. Finally, an esterification reaction between anhydride and hydroxyl groups is used to introduce quaternary phosphonium salt and silver citrate structures into the molecular chain, resulting in a novel, highly efficient bactericide. The novel, highly efficient bactericide prepared by this invention simultaneously contains quaternary ammonium salt, quaternary phosphonium salt, and metallic silver ions. The positively charged quaternary ammonium salt and quaternary phosphonium salt are adsorbed onto the negatively charged bacterial surface through electrostatic interactions, exerting antibacterial and bacteriostatic effects by altering membrane permeability. The silver ions in silver citrate can bind to the sulfhydryl and carboxyl groups of bacterial membrane proteins, disrupting membrane integrity and thus exerting antibacterial and bacteriostatic effects.

The application discloses an immunoregulatory antibacterial peptide targeting LPS and a preparation method and application thereof, and belongs to the technical field of biology. The amino acid sequence of the antibacterial peptide FF2 is shown as SEQ ID No. 1, and the antibacterial peptide FF2 of the application can specifically target the bacterial membrane surface component LPS to make the bacterial membrane leak and inhibit the growth of bacteria. The antibacterial peptide can capture bacteria and trigger the innate immune system to achieve the antibacterial purpose, and can also target the bacterial membrane to break the membrane and kill bacteria, thereby fully avoiding the killing of beneficial bacteria in the host body, avoiding the overuse of the antibacterial peptide, effectively reducing the generation of drug resistance, and mobilizing the immune function of the body to synergistically resist bacterial infection. In conclusion, the antibacterial peptide FF2 is a targeted LPS forming nanofiber antibacterial peptide with immunoregulatory function, which has great development potential and high application value.

The invention provides a rapamycin-loaded engineered bacterial nano-vesicle, a preparation method and an application thereof, and belongs to the field of biological medicines and nano preparations, the rapamycin-loaded engineered bacterial nano-vesicle is of a hollow spherical morphology and has a phospholipid bilayer structure, fat-soluble rapamycin is embedded between phospholipid bilayers, and the surface is coated with a layer of calcium phosphate mineralized shell to form AbNV / Rapa CaP. The preparation method comprises the following steps: extracting bacterial membrane components, co-incubating with rapamycin, extruding to form uniform nano-vesicles AbNV / Rapa, and coating the nano-vesicles with a pH-responsive calcium phosphate phosphorite coating through the steps of centrifugation and mineralization to finally obtain the complete AbNV / Rapa-coated CaP. The compound is applied to preparation of anti-tumor drugs. According to the invention, the macrophages can be promoted to phagocytize tumor cells from the aspects of repolarization and anti-aging of the tumor-related macrophages; the preparation method is reasonable in design and simple in preparation process, has a wide application prospect, and lays a foundation for design and development of a corresponding drug delivery system.

The invention discloses an antibacterial material with calcium ion mediation and photo-thermal effects as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. And the antibacterial material is a calcium ion loaded porous covalent organic framework polymer BQ-Ca-COF. The preparation method comprises the following steps: carrying out solvothermal reaction on 2, 3, 5, 6-tetraaminocyclohexane-2, 5-diene-1, 4-diketone and cyclohexanehexone octahydrate to obtain a porous covalent organic framework polymer; and under a protective atmosphere, carrying out metallization reaction on the porous covalent organic framework polymer and calcium chloride to obtain the BQ-Ca-COF. The BQ-Ca-COF prepared by the invention has a synergistic effect of controllable release of Ca < 2 + > and photo-thermal irradiation, has good photo-thermal conversion under irradiation of laser with the wavelength of 638 nm, and can release calcium ions under irradiation of infrared (NIR) laser, increase the permeability of a bacterial membrane and synergistically enhance the efficiency of subsequent photo-thermal action, so that gram positive and negative bacteria are effectively killed.

The present application relates to a kind of bactericide bactericidal performance evaluation method and evaluation system, belong to the field of oil engineering application technology.The bactericide bactericidal performance evaluation method of the present application, the basic skeleton of bacterial membrane is constructed by coarse-grained molecular dynamics method in nanometer space scale with phospholipid bilayer, and the choline group and phosphate group of head group make the surface of bacteria positive and negative, bactericide is adsorbed to the surface of bacteria by interacting with the charged group on the surface of bacteria, by comparing the binding energy of bactericide and bacteria, whether bactericide can be stably adsorbed on the surface of bacteria and hinder the normal metabolism of bacteria, and finally kill bacteria can be evaluated.The bactericide bactericidal performance evaluation method of the present application does not need to pass through traditional experiment, and is evaluated using computer "virtual experiment", is not limited by experimental condition, is high in efficiency, is strong in repeatability, result is reliable, is conducive to the rapid design and research and development of bactericide for oil field chemical.

The invention discloses an antibacterial material for root canal therapy based on fluorine titanium oxide and a preparation method and application of the antibacterial material, and belongs to the technical field of oral medical materials, and the antibacterial material for root canal therapy is fluorine titanium oxide nanoparticles; the fluorine titanium oxide nanoparticles are fluorine-doped titanium oxide; the fluorine titanium oxide nanoparticles can absorb infrared radiation of a human body and generate surface electromagnetic oscillation, so that a bacterial membrane structure is destroyed through a charge polarization effect. A continuous antibacterial field is excited on the surfaces of the fluorine titanium oxide nanoparticles by utilizing human body infrared radiation, the antibacterial effect of destroying a bacterial membrane by charge polarization is realized, and the method has the advantages of long-acting antibacterial effect, no chemical release, avoidance of cytotoxicity and the like; in addition, when the antibacterial material fluorine titanium oxide nanoparticles are prepared into antibacterial coating liquid, the operation process can be simplified, and the antibacterial coating liquid is suitable for clinical root canal flushing instruments.

A biocidal composition, comprising an antibacterially effective combination of IPBC, zinc oxide, and bacterial membrane disrupting substances, and a working composition comprising water, one or more organic compounds, and a biocidal composition comprising IPBC, zinc oxide, and a bacterial membrane disrupting substance, effective for wet-state and dry-film preservation of the working composition. Also, a method of enhancing antibacterial activity of IPBC in a wet-state working composition comprising water and one or more organic compounds, comprising treating the working composition with IPBC in the presence of zinc oxide and bacterial membrane disrupting substances, and a method of treating an aqueous working formulation containing one or more organic compounds, to prevent, inhibit, or reduce wet-state bacterial contamination of the formulation, comprising treating the working formulation with a biocidal composition comprising an antibacterially effective combination of IPBC, zinc oxide, and a bacterial membrane disrupting substance.

The invention relates to the field of bacterial membrane protein structures. More specifically, the invention relates to lipid nanodiscs compartmentalized by SlyB protein oligomers isolated from the outer membrane of Gram-negative bacteria. More specifically, the invention provides for a SlyB nanodisc structure wherein the SlyB-oligomer forms the membrane scaffold protein belt, which is surrounded by outer saccharolipid moieties anchored to the SlyB proteins, and which encloses a lipid bilayer nanodomain containing one or more phospholipid layers, wherein macromolecules such as (outer) membrane protein molecules may be captured and stabilized. More specifically, methods to produce and isolate chemically defined stable SlyB nanodisc particles are disclosed herein. Finally, the invention relates to the use of said SlyB nanodiscs as a self-adjuvanting vehicle, as part of an immunogenic composition, and provides for novel means for use in eliciting an immune response against macromolecules enclosed in said SlyB nanodiscs, or for use in a vaccine composition.

The present invention discloses a method for removing extracellular DNA contamination from wastewater. The method primarily comprises the following steps: filtering the original wastewater and performing ultrasonic treatment to remove impurities and disrupt bacterial membranes to release DNA; mixing a predetermined amount of carbon nanotubes with the wastewater; adsorbing the extracellular DNA onto the carbon nanotube surfaces; and, after adsorption equilibrium has been reached, adding a predetermined concentration of persulfate to initiate an oxidation reaction, generating reactive oxygen species that oxidatively degrade the extracellular DNA in the wastewater. Compared to existing methods for removing extracellular DNA contamination, the present invention offers the advantages of simplicity, high efficiency, low construction cost, and wide application. It can remove extracellular DNA from wastewater, thereby controlling the risk of resistance gene transmission in the wastewater.

The invention discloses a guanidyl chitosan derivative capable of destroying a bacterial membrane as well as a preparation method and application of the guanidyl chitosan derivative, and belongs to the field of antibacterial materials. The derivative is prepared through four steps of reaction: reacting N, N-di-Boc-S-methylisothiourea with ethanolamine to prepare OBG; then, the OBG and lipoic acid are esterified under the catalysis of DCC / DMAP to obtain LBG; the preparation method comprises the following steps: reacting chitosan with N-acetylcysteine under the action of EDCI / HOBT to prepare CSSH; finally, LBG is subjected to trifluoroacetic acid deprotection to generate LGa, LGa and CSSH react in a hydrochloric acid-triethanolamine buffer solution and DMF mixed solution, and the guanidyl chitosan derivative is obtained. The derivative can adsorb negatively charged bacteria through electrostatic interaction, tear and destroy a bacterial membrane structure, effectively kill methicillin-resistant staphylococcus aureus and pseudomonas aeruginosa, is suitable for the field of biological sterilization, and provides support for development of novel antibacterial materials.

The invention discloses an LPS-targeted immunoregulation antibacterial peptide as well as a preparation method and application thereof, and belongs to the technical field of biology. The amino acid sequence of the antibacterial peptide FF2 is shown as SEQ ID No.1. The antibacterial peptide FF2 can specifically target a bacterial membrane surface component LPS, so that the bacterial membrane leaks, and bacterial growth is inhibited. The antibacterial aim can be achieved when bacteria are captured and a congenital immune system is triggered, membrane rupture sterilization can be carried out by targeting a bacterial membrane, killing of beneficial bacteria in a host body is fully avoided, meanwhile, excessive use of antibacterial peptide is avoided, generation of drug resistance is effectively reduced, and the immune function of the body is mobilized to resist bacterial infection in a synergistic mode. In conclusion, the antibacterial peptide FF2 is a nanofiber antibacterial peptide formed by targeting LPS, which has great development potential and relatively high application value and has an immunoregulation function.

The application discloses a kind of fat cationic amphiphilic peptide mimics and its preparation method and application.The application first develops a kind of cysteine-based amphiphilic cationic peptide mimic system, then, by forming peptide bond at carboxyl terminal, introduce ethylenediamine as cationic group, while introducing aromatic ring-containing hydrophobic group in thiol side chain;Among them, cationic group provides positive charge and hydrophilicity, helps to carry out electrostatic interaction with the bacterial membrane with negative charge, while hydrophobic group helps membrane insertion phospholipid bilayer;In addition, the application tunes the amphiphilic balance of cysteine-based cationic peptide mimic by modifying N-terminal with different chain length fatty acids, and finally synthesizes fat cationic amphiphilic peptide mimic.The peptide mimic has good antibacterial activity, and is not easy to induce bacteria to produce drug resistance, and can be used for preparing drugs for treating, improving and / or preventing drug-resistant bacteria and / or drug-resistant fungal infection.

The invention belongs to the technical field of medicines, and provides a preparation method of Rosella mucilaginosa vesicle composite gel aiming at the problem that application of bacterial membrane vesicles to treatment of actinic cheilitis is not researched yet at present, the preparation method comprises the following steps: S1, obtaining Rosella mucilaginosa; s2, preparation of Rosella mucilaginosa vesicles; and S3, preparation of the Rosella mucilaginosa vesicle composite gel. According to the invention, human symbiotic bacteria are used for culture and extraction, the active product has good biological safety and low sensitization, the vesicle treatment is easier to absorb, and the infection risk caused by direct use of viable bacteria is avoided; therefore, the Rosella mucilaginosa vesicle composite gel provided by the invention is beneficial to maintaining the health of the lips, resisting oxidative stress, reducing the generation of active oxygen, relieving porphyrin and iron overload of local tissues caused by ultraviolet rays, and improving the lip health. And a constructed rat actinic cheilitis model verifies that the Rosella mucilaginosa vesicle composite gel disclosed by the invention can be used for preventing and treating actinic cheilitis.

The invention relates to novel application of chlorothiazide, in particular to a bacterial cell membrane bionic nano-drug loaded with an ROS responsive chlorothiazide prodrug and application of the bacterial cell membrane bionic nano-drug. The invention relates to novel application of chlorothiazide and bionic nano-drugs in preparation of drugs for treating inflammatory bowel diseases. The inventor finds for the first time that the Chl active compound can directly drive phenotype reversal of macrophages from M1 to M2 and synchronously repair intestinal mucosal barriers, and breaks the pathogenic closed loop of barrier destruction-inflammation aggravation; however, the colon targeting property is poor, the water solubility is low, and the whole body exposure is limited. Therefore, according to the application, Chl is transformed into an ROS responsive prodrug (CH NPs), and the ROS responsive prodrug (CH NPs) is coated with a cell membrane of an escherichia coli Nissle1917 strain, so that the bionic nanoparticles (CHM NPs) are constructed. The system not only retains the ROS responsiveness of CH NPs, but also obviously improves the targeting ability and residence time of the nanoparticles in a colon area by virtue of the biological characteristics of the bacterial membrane.

Disclosed is a synthetic β-hairpin antimicrobial peptide (AMP) comprising (a) a recognition module comprising a hairpin turn for interacting with a bacterial membrane component to initiate amyloid nucleation; and (b) a structural module comprising a first side strand and a second side strand for β-sheet formation and molecular stacking during fibrillation; wherein the synthetic β-hairpin AMP comprises the sequence of SEQ ID NO: 1 or SEQ ID NO: 2; and wherein the synthetic β-hairpin AMP self-assembles into nanonets in the presence of the bacterial membrane component or a bacterium. Also disclosed is a pharmaceutical or adjuvant composition comprising the AMP. Also disclosed is a method for treating a condition associated with a bacterial infection, or an immune-related disease, or for delaying the development of antibiotic resistance in a subject in need thereof using the AMP or composition.

The invention belongs to the technical field of biological application, and particularly relates to an antibacterial peptide and application thereof, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The invention provides an antibacterial molecule with a novel structure, the antibacterial molecule is composed of 28 amino acids and has no significant homology with reported mussel antibacterial peptide sequences such as Mytilin and Myticin, the antibacterial peptide enriches an antibacterial peptide molecular library, the theoretical isoelectric point of the polypeptide is up to 11.72, the net charge is + 4, the polypeptide has high positive charge density and a hydrophobic amino acid proportion of 53.6%, and the antibacterial peptide has a good application prospect. The amphiphilic structural characteristics are obviously better than those of the traditional antibacterial peptide, and the antibacterial peptide is endowed with the capability of efficiently targeting a bacterial membrane. Bacteriostatic experiments prove that the inhibition rate on escherichia coli reaches 60%, the inhibition rate on staphylococcus aureus is 20%, obvious concentration dependence and strain specificity are shown, the effect is accurate, and broad-spectrum drug resistance is not likely to be induced.

The invention discloses an artificially designed antibacterial peptide PEP1 and application thereof. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID No.1, the net charge is + 7, and the secondary structure is predicted to be stable alpha-helix. According to the design of the antibacterial peptide, the flexibility of a peptide chain is enhanced through N-terminal glycine, charges are finely adjusted through C-terminal glutamic acid to optimize selectivity, and an imperfect amphiphilic interface beneficial to targeting a bacterial membrane is formed. An in-vitro antibacterial activity experiment shows that the antibacterial peptide has the characteristic of rapid sterilization; the research on the action mechanism shows that by destroying bacterial cell membranes and further combining genomic DNA, the double sterilization effect is achieved; safety evaluation shows that the antibacterial peptide shows excellent biocompatibility. The antibacterial peptide can be used for preparing an anti-bacterial infection preparation, can also be applied to the fields of antibacterial material development, cosmetics, food preservation, animal feed additives, animal and plant disease prevention and treatment and the like, has efficient antibacterial activity and good biological safety, and is not easy to induce bacterial drug resistance.

The invention discloses a deuterated antibacterial peptide as well as a composition and application thereof, and belongs to the field of biological medicines. By introducing deuterated 2-aminobutyric acid to form an annular structure and combining deuterated modification, the affinity of the antibacterial peptide to a bacterial membrane is greatly improved compared with that of traditional drugs (polymyxin B and D50), and the selectivity of the antibacterial peptide to a mammalian membrane is 884 times or above higher than that of a control; the minimum inhibitory concentration to gram-negative bacteria in vitro is as low as 0.015 mu g / mL, which is increased by 67 times compared with polymyxin B, is increased by 33-67 times compared with D50 control peptide, and is increased by about 17 times compared with non-deuterated antibacterial peptide 5, so that extremely-low-concentration efficient bacteriostasis is realized; the toxicity to human kidney cells is extremely low, and the biological safety is better. In a plurality of infection models, the treatment effect of the compound is obviously better than that of the three control substances, and the compound can be used for preparing anti-infection drugs, provides a new scheme of high efficiency and low toxicity for clinical anti-infection treatment, and is suitable for prevention and treatment of a plurality of infections.

The invention belongs to the field of design and synthesis of small organic molecules, and discloses a triphenylamine quaternary ammonium salt derivative antibacterial agent as well as a preparation method and application thereof. According to the invention, triphenylamine is taken as an electron donor, phenol is taken as an electron acceptor, a series of triphenylamine quaternary ammonium salt derivatives (TPQs) with alkyl chains with different lengths are synthesized, and the influence of the alkyl chain lengths on antibacterial activity is researched. The cation of the compound is combined with a negatively charged bacterial membrane, the amphipathy is adjusted by a hydrophobic alkyl chain, the visualization of an interaction mode between TPQs and bacteria is disclosed by the excellent fluorescence property, and the photodynamic antibacterial activity is enhanced by ROS. System research finds that the antibacterial activity of a short chain is higher than that of a long chain. The TPQ-1 has excellent antibacterial activity while keeping short-chain low cytotoxicity, and is a short alkyl chain cationic amphiphilic compound with good application prospects. The invention provides a new thought for designing an antibacterial agent with good biocompatibility. # imgabs0 #

This invention discloses the use of a bacterial membrane dCACHE-type chemokine receptor protein family as a target in the preparation or screening of drugs against multidrug-resistant bacteria, belonging to the field of biomedical technology. This invention reveals and verifies for the first time the core regulatory mechanism of the dCACHE-type chemokine receptor protein family in the bacterial infection process: these proteins are key membrane surface receptors mediating bacterial chemotaxis towards macrophages; their loss of function can directly block bacterial chemotactic movement and significantly reduce pathogenicity. This invention, by targeting the conserved ligand binding interface, successfully inhibited the infectivity of drug-resistant pathogens such as *Yersinia pseudotuberculosis*, carbapenem-resistant *Acinetobacter baumannii*, and carbapenem-resistant *Escherichia coli*. The novel targeting strategy provided by this invention not only effectively overcomes the clinical bottleneck of "no available drugs" for carbapenem-resistant multidrug-resistant bacteria but also constructs a broad-spectrum protective barrier covering the entire homologous protein family, possessing extremely high clinical translational value and commercial development potential.

The present invention discloses a 16-amino acid polypeptide AA16 extracted from the LPS-binding protein CD14. 13 derivative polypeptides are obtained by amino acid replacement to increase α-helicity, improve amphiphilicity, and change the number of positive charges and hydrophobicity, thereby obtaining a targeting peptide with specific resistance to Gram-negative bacteria. Among them, the derivative polypeptide AA16-10R is specifically resistant to Gram-negative bacteria, with minimum inhibitory concentrations of 32 μg / mL and 16 μg / mL for Pseudomonas aeruginosa and Escherichia coli, respectively. It can bind to LPS on the bacterial membrane in the form of an α-helix, increase membrane permeability, and ultimately cause bacterial death, thereby having a good antibacterial effect.

The invention relates to the technical field of antibacterial coatings, in particular to a titanium-based implant antibacterial coating, a preparation method of the titanium-based implant antibacterial coating and a prepared titanium-based implant. The coating is a Bi-TCPP compound formed by coordination bonding of bismuth ions and tetra (4-carboxyphenyl) porphyrin, and is covalently fixed on the surface of the titanium-based implant through a silane coupling agent layer. The preparation method comprises the following steps: performing cleaning and alkali heat treatment on a titanium substrate to construct an active surface; grafting a coupling agent layer through silanization reaction; the preparation method comprises the following steps: preparing a Bi-TCPP compound by a solvothermal method; and immobilizing the compound on the surface of a substrate through amidation reaction. According to the coating, efficient sterilization is achieved through pseudo-iron metabolism interference of bismuth ions and the double synergistic effect of TCPP on a bacterial membrane electron transfer chain, selective antibiosis is achieved through the structural difference between bacteria and human cells, and cytotoxicity is effectively avoided. The coating has the ultrasonic response characteristic, on-demand controlled release of antibacterial factors can be achieved, and the antibacterial efficiency and durability are remarkably improved.

The invention belongs to the technical field of biological medicine, and particularly relates to an apatinib-loaded engineered bacterial membrane vesicle and a preparation method and application thereof.The engineered bacterial membrane vesicle is prepared by mixing apatinib and a membrane vesicle derived from rodent bacillus in a PBS according to a specific mass ratio; incubating at 37 DEG C for 4 hours, removing free drugs by using a 100kDa ultrafiltration membrane, and concentrating; and washing with PBS to obtain a final product. Experimental results show that the engineered bacterial membrane vesicles can effectively inhibit malignant biological behaviors such as proliferation of breast cancer cells and significantly inhibit tumor growth in an animal model; mechanism research shows that the compound can effectively activate the anti-tumor immune response of the body; safety evaluation shows that the engineered bacterial membrane vesicle has no toxic or side effect; in conclusion, the engineered bacterial membrane vesicle provided by the invention has dual advantages of chemotherapy and immunotherapy, is good in safety, and has important application value in development of breast cancer treatment drugs.

The invention relates to an evaluation method and an evaluation system for the sterilization performance of a bactericide, and belongs to the technical field of petroleum engineering application. According to the method for evaluating the bactericidal performance of the bactericide, a basic skeleton of a bacterial membrane is constructed by a phospholipid bilayer in a nano spatial scale through a coarse graining molecular dynamics method, and the choline group and the phosphate group of the head group enable the bacterial surface to be positively charged and negatively charged; the bactericide is adsorbed to the surface of bacteria through interaction with charged groups on the surface of the bacteria, and whether the bactericide can be stably adsorbed on the surface of the bacteria so as to hinder normal metabolism of the bacteria and finally kill the bacteria can be evaluated by comparing the binding energy of the bactericide and the bacteria. According to the method for evaluating the sterilization performance of the bactericide, traditional experiments are not needed, a virtual experiment of a computer is used for evaluation, the method is not limited by experimental conditions, efficiency is high, repeatability is high, results are reliable, and rapid design, research and development of the bactericide for oilfield chemistry are facilitated.