31results about How to "Increased separation cost" patented technology

The invention provides a comprehensive utilization method of corn cob, which effectively separates hemicellulose, lignin and cellulose contained in a reproducible biological resource of corn cob in a reasonable process order at low cost and high efficiency, and makes full use of every type of biomass components, thereby solving the problem of utilization of residue in the biomass refining process of preparing ethanol and butanol by extracting xyio-oligosacchandes and enzymolysis from the corn cob in the prior art, and making reasonable and effective use of main components of biomass contained in the corn cob; particularly, the lignin can substitute partial petrochemical materials to prepare modified high-molecular materials, thereby reducing the cost of high-molecular materials and saving the energy consumption of petrochemical materials to bring great social benefit; in addition, the comprehensive utilization method of corn cob is featured by simple processing technique, easy implementation and favorable effect.

The invention provides an application of a lipase in splitting N-acetyl-DL-methionine methyl ester, the amino acid sequence of the lipase is shown in SEQ ID NO.1, and its coding gene is shown in SEQ ID NO .2 shown. The lipase gene of the present invention can be connected with an expression vector to construct an intracellular expression recombinant plasmid containing the gene, and then transformed into an E. coli strain to obtain a recombinant E. coli engineering bacterium; the recombinant E. coli is subjected to cell crushing, separation and purification to obtain a recombinant fat Enzyme; the recombinant lipase has the ability to catalyze the splitting of N-acetyl-DL-methionine methyl ester, and can obtain N-acetyl-L-methionine methyl ester with an enantiomeric excess value of >99% and a conversion rate of 51.2 %.

The present invention discloses a 5-benzyloxy-4-oxo-4H-pyran-2-carboxylic acid preparation method comprising the following steps: adding kojic acid, benzyl chloride and methanol benzyl chloride into a reaction vessel, and heating for reflux reaction for 16 to 18 hours; distilling under reduced pressure, evaporating the solvent to dryness, and cooling to room temperature; adding methanol and water, stirring, washing, filtering and drying to obtain an intermediate; and adding the intermediate and water into the reaction vessel, reducing the temperature to 0 DEG C to -10 DEG C, adding sodium periodate, controlling the temperature to be not higher than 5 DEG C, then adding dropwise a proper amount of water, stirring for 5-15 minutes, heating to 12 DEG C-22 DEG C, stirring for reaction for 2.5 to 3.5 hours, extracting with ethyl acetate, layering, drying, filtering and drying to obtain white solid 5-benzyloxy-4-oxo-4H-pyran-2-carboxylic acid. The new 5-benzyloxy-4-oxo-4H-pyran-2-carboxylic acid preparation method is provided, does not produce large amounts of chromium-containing waste water, prevents severe environmental pollution, and can achieve industrial production.

A method for preparing high-purity 1-pentene. The process includes: using the raffinated C5 after diolefins and isopentene have been extracted and separated as raw material for rectification and separation to remove heavy components, the top of the tower is enriched with 1-pentene cuts, and the heavy components are discharged from the bottom of the tower; by process 1 ) The fraction enriched in 1-pentene obtained at the top of the tower is subjected to precise rectification to remove light components, and the top of the tower obtains carbon penta-alkanes and other light components, and the crude product of 1-pentene is obtained in the bottom of the tower; by process 2) The crude product of 1-pentene is etherified with methanol to remove the difficult-to-separate 2-methyl-1-butene in the crude product; the ether product obtained in process 3) is sent to a refining tower for purification to obtain More than 98% 1-pentene. The invention improves the process flow, simplifies the process, reduces the production cost, and improves the economic efficiency of the process for preparing high-purity 1-pentene.

The invention belongs to the biomedical field and particularly belongs to the field of stem cells. The invention provides a method of separating UC-MSCs (umbilical cord mesenchymal stem cells). The method comprises the following steps: 1) mixing umbilical cord tissue pieces with pancreatin and collagenase to incubate; and 2) adding hyaluronidase to incubate.

The invention discloses a catalyst for preparing 1,3,3,3-tetrafluorohydrazine propylene from 1,1,1,3,3-perfluoropropane. The composition is MxOy / AlF3, wherein a carrier AlF3 is an active carrier which is of an alpha phase structure, has the specific surface area greater than 150 m<2>.g<-1> and has the aperture distribution concentrated at 15 to 40 nm; MxOy is one of MgO, Fe2O3, Co2O3 or CuO; the mole ratio of MxOy to AlF4 is 1: (5 to 50). A preparation method of the catalyst comprises the following steps: carrying soluble salt of any element of Mg, Fe, Co or Cu through active alpha-phase AlF3 which has a large specific surface area and a mesoporous structure and serves as the carrier via an equivalent immersion method, and preparing the catalyst through drying and high temperature roasting. The catalyst disclosed by the invention is excellent in performance; in a preparation process, no expensive noble metal compounds are needed, the cost is low, and the activity is high; during reaction at 280 DEG C, the conversion ratio of the 1,1,1,3,3-perfluoropropane is 70 percent or above, and the catalyst is long in service life and can stably work for 360 hours; meanwhile, no diluting gas is fed in the reaction process, and the catalyst has industrial application value.

The method for producing high-purity 1-pentene, the process includes: using the raffinate carbon five after extraction and separation of diolefins and isopentene as raw material to rectify and separate and remove heavy components, and enrich 1-pentene at the top of the tower Distillate, the heavy component is discharged from the tower bottom; the fraction enriched in 1-pentene obtained from the process 1) at the top of the tower is subjected to precise rectification to remove light components, and carbon penta-alkanes and other light components are obtained at the top of the tower, and obtained at the bottom of the tower The crude product of 1-pentene; the crude product of 1-pentene obtained by process 2) isomerized through a fixed-bed catalytic bed of sulfonic acid-based cation exchange resin, so that the difficult-to-separate 2-methyl-1-in the crude product Butene is converted into 2-methyl-2-butene which is easier to separate; the isomerization product obtained in process 3) is sent to a refining tower for purification to obtain more than 98% 1-pentene. The invention improves the process flow, simplifies the process, reduces the production cost, and improves the economic efficiency of the process for preparing high-purity 1-pentene.

The invention provides a comprehensive utilization method of corn cob, which effectively separates hemicellulose, lignin and cellulose contained in a reproducible biological resource of corn cob in a reasonable process order at low cost and high efficiency, and makes full use of every type of biomass components, thereby solving the problem of utilization of residue in the biomass refining process of preparing ethanol and butanol by extracting xyio-oligosacchandes and enzymolysis from the corn cob in the prior art, and making reasonable and effective use of main components of biomass contained in the corn cob; particularly, the lignin can substitute partial petrochemical materials to prepare modified high-molecular materials, thereby reducing the cost of high-molecular materials and saving the energy consumption of petrochemical materials to bring great social benefit; in addition, the comprehensive utilization method of corn cob is featured by simple processing technique, easy implementation and favorable effect.

The invention provides a device for removing volatile odor from a sludge protein solution. The device for removing the volatile odor from the sludge protein solution comprises an evaporating device, a rectifying device and a odorant liquid receiving device, wherein the evaporating device comprises a temperature controller, a tower kettle and a heater on the outer side of the tower kettle; a thermoresistor thermometer is arranged inside the tower kettle; the thermoresistor thermometer interacts with the temperature controller; the heater is connected with a heat carrier pipe; a pneumatic actuator is arranged on the heat carrier pipe; the pneumatic actuator is connected with the temperature controller; a rectifying interface is further formed in the tower kettle; the rectifying interface is connected with the rectifying device; the rectifying device is connected with the odorant liquid receiving device. The device for removing the volatile odor from the sludge protein solution, provided by the invention, does not destroy sludge protein and reduce the purity thereof while sludge protein deodorization is achieved.

The invention discloses a preparation method of biurea, wherein the preparation method comprises the steps: firstly, preparing a 20%-25% hydrazine hydrate solution as a raw material; followed by, adding a urea solution into a condensation kettle in a successive subsection manner, and carrying out condensation reaction, wherein the mass ratio of hydrazine hydrate to urea is less than or equal to 1:2.55, and the addition way of the urea solution is divided into three sections; after addition of urea is completed, continuing to carry out reaction for a period of time until the concentration of residual hydrazine hydrate in a reaction liquid in the condensation kettle is less than or equal to 1 g / L, stopping heating of the condensation kettle, and then adding water to dissolve sodium chloride precipitated in the condensation reaction process; and cooling, and then successively carrying out discharging, solid-liquid separation, and washing and drying treatment to obtain the diurea product. The preparation method of the diurea makes the preparation process of the diurea more energy saving and more efficient and makes the cost lower, impurities in the prepared diurea are fewer, and the purity is higher.

The invention provides a bidirectional magnetic projection automatic separation device for plastic waste. The bidirectional magnetic projection automatic separation device comprises a separation box used for containing a medium solution. the bidirectional magnetic projection automatic separation device further comprises a feeding mechanism and an axial magnetizing annular magnet group, the axial magnetizing annular magnet group is arranged between the feeding mechanism and the separation box, the feeding mechanism comprises a feeding channel and a pendulum bob, wherein the discharging end of the feeding channel penetrates through the annular magnet group and communicates with the separation box, the pendulum bob is arranged in the feeding channel in a sliding mode and can push materials in the feeding channel to the separation box, the end face of the discharging end of the feeding channel is perpendicular to the central axis direction of the annular magnet group, the end face of the pushing end of the pendulum bob is of a molded surface structure consistent with the end face of the discharging end, and the center of the end face of the discharging end is close to or located on the central axis of the annular magnet group. The bidirectional magnetic projection automatic separation device is high in separation precision, high in separation recovery rate and large in single-time separation amount, a mixture of various plastic wastes can be separated, the working efficiency is greatly improved, and the separation cost is reduced.

The invention provides a method for separating and extracting squalene in mixed tocopherols. The method comprises steps of: filling silochrom with the meshes of 100-200 into a chromatographic column, adopting an elution solvent to elute and compact the silochrom, then feeding the tocopherols into the chromatographic column according to a proper ratio, and separating the squalene from natural vitamin E through utilizing column chromatography. By adopting the separating and extracting method, the technological process is simple, the purity and recovery rate of the squalene can be improved to the maximum extent through utilizing a gradient elution method, silicagel columns can be well regenerated through utilizing strong polar solvents such as methyl alcohol and the like, and the method has the advantages of large solvent usage, easiness in recycling, small consumption and small environmental pollution.

The invention provides a method for separating gas. The method comprises the following steps of: (1) building enclosed wall bodies at all exits of an isolated worked out section to fully isolate the worked out section from the outside; (2) arranging a gas inlet pipeline in at least one enclosed wall body on the lower part of the worked out section; (3) connecting pumped gas with the gas inlet pipeline at the inner side of the enclosed wall body through a fire arrestor at the outer side of the enclosed wall body; (4) forming at least one drill hole in a fissure zone on the upper part of the worked out section to collect enriched gas; and (5) connecting the drill hole with a gas outlet pipeline through the fire resistor. By adopting the method, huge inbreak space of the underground worked out section is fully utilized, natural separation of gas from the air is realized by using the property that gas is lighter than the air under the condition of low separating cost, the concentration oflow-concentration gas with high separation cost by using a manual device is increased to be over than 40 percent, and 100 percent separation and purification of gas pumped from a mine is realized.