49results about How to "Method directly" patented technology

The invention provides a temporomandibular joint movement reconstruction method and a system thereof. The method is characterized by collecting an image sequence of a mark point track of open and close mouth mandible movements of a photographed person; determining three-dimensional coordinates of all the mark points on an intraoral fixed mark device in each frame of image, acquiring a movement track of each mark point in a space, and collecting a CBCT image of the intraoral fixed mark device and collecting a CBCT image of the photographed person; registering the mark point of the CBCT image of the intraoral fixed mark device with the mark point acquired by a camera through photographing, and acquiring a transformation matrix of a mark point movement; and using the transformation matrix to carry out rigid body transformation on the CBCT image of the photographed person, acquiring a CBCT image sequence of the photographed person and acquiring a temporomandibular movement image. In the invention, real movement conditions of mandible condyle process and a temporal bone joint surface of a human-body temporomandibular joint can be acquired, the method and the system can be used for auxiliary diagnosis of a temporomandibular joint disease and dynamic analysis of joint motion, temporomandibular joint diagnosis means are increased, doctor diagnosis accuracy and efficiency can be increased, and medical quality and efficiency can be increased too.

This invention provides primers for in vitro diagnosis of mutation of autosomal recessive nonsyndromic hearing loss gene GJB2. The primers can be used for completely amplifying the coding region and upstream or downstream important splicing sequences of GJB2 gene. This invention also provides a test kit for containing the primers and ApaI restriction endonuclease and their application for in vitro detection of 233-235delC mutation of the complete GJB2 coding region. The test kit can also be used for detecting the mutation sites of the GJB2 coding region through sequencing by the forward primer, and detecting deletion or insertion heterozygous mutation through sequencing by the reverse primer. The test kit is suitable for large-scale screening of autosomal recessive nonsyndromic hearing loss gene GJB2 mutation and genetic counseling.

The invention discloses a telescope secondary mirror position correcting method based on optical spot definition function. The method comprises the following steps of 1, obtaining the position of a secondary mirror with objective function having an extreme value by taking onaxis field-of-view optical spot definition as the objective function, translation of the secondary mirror along the direction of an optical axis and translation and rotation of the secondary mirror along two orthogonal directions vertical to the optical axis direction through an iterative algorithm, wherein if all the variables are simultaneously iterated, the objective function is likely to be converged to a local extreme value, so that each iterative process is enabled to comprise two steps of iteration of the optical axis direction and the direction vertical to the optical axis direction so as to enable the objective function to be converged to a global extreme value; 2, enabling the secondary mirror to rotate around a zero comet difference point of a system along the two orthogonal directions and searching for the extreme value of the objective function in an iterative manner for realizing correction for the relative position of the secondary mirror by taking the mean value of definition function of multiple axis external field-of-view optical spots as the objective function. The method is free from wavefront detection and reestablishment, adjustment for a telescope system has an objective standard, and the telescope imaging quality can be monitored in real time.

The invention discloses an avalanche photodiode (APD) gain stabilization control device and an APD gain stabilization control method. The device consists of a bias control circuit, an APD detection circuit, a data acquisition card and a computer; and the control method comprises the following steps that: the computer directly judges difference of an average digital signal acquired by the data acquisition card and a set signal value and transmits an APD bias regulating command, and the APD bias voltage is fed back and controlled through the bias control circuit; the whole device forms a closed loop feedback system; and the APD amplifier gain is finely adjusted so as to fulfill the aim of stabilizing the acquired digital signal. The invention has the characteristics that: the complexity and unreliability of a method for controlling APD temperature to be constant or a temperature-based feedback bias control method are avoided, the stability and reliability of the acquired digital signal can be easily and effectively improved, and the method is direct, effective, simple and reliable and has high practical and commercial values.

The invention relates to a nucleic-acid aptamer type photoelectric sensor for detecting atrazine and a preparation method of such nucleic-acid aptamer type photoelectric sensor. The preparation method includes firstly, preparing a three-dimensionally ordered macroporous 3DOM TiO2 / FTO electrode; then, performing Au nanoparticle modification through a hydrothermal reduction method so as to obtain an Au NPs / 3DOM TiO2 / FTO composite electrode; finally, combining an atrazine aptamer onto the surface of the Au NPs / 3DOM TiO2 / FTO composite electrode through an Au-S bond self-assembled film method so as to obtain an Aptamer / Au NPs / 3dom TiO2 / FTO sensor electrode. Compared with the prior art, the nucleic-acid aptamer type photoelectric sensor for detecting the atrazine and the preparation method have the advantages that the nucleic-acid aptamer is used as an atrazine recognition element, so that sensor detection selectivity is improved greatly; since a three-dimensionally ordered macroporous Au NPs / 3DOM TiO2 / FTO composite material of a microstructure is used for recognizing the molecular load of nucleic-acid aptamer element, the photoelectric catalysis performance can be enhanced effectively, atrazine detection sensitivity is improved, the limit of detection is as low as ng / L, high selective recognition is achieved, and the nucleic-acid aptamer type photoelectric sensor can be applied to detection and analysis of trace pollutants accordingly.

The invention discloses a method for predicting maximum height of a filling mining diversion fissure zone. Supposing a precondition that no caving zone is generated and only a fissure zone and a sagging zone are generated in the advancing process of a working face, a mathematical model which is used for predicting the maximal height of the filling mining diversion fissure zone; a critical value of top board deformation is used for determining the strata failure low of an over-limit stress limited space; and whether the strata fails is determined according to the critical value of the top board deformation. Therefore, compared with the prior art, the method has advantages of more direct calculation and higher convenience. A calculation formula for predicting the maximum height of the filling mining diversion fissure zone can be simply and accurately obtained. Furthermore the parameters in the formula can be easily acquired, and higher accuracy of the calculation process can be realized. Simple and direct calculation can be obtained. The method can quickly, effectively and accurately predict the maximum height of the diversion fissure zone and is suitable for guidance for actual production, thereby realizing safe mining under water body.

The invention relates to a metallogenic prediction method based on fuzzy logic and machine learning. The method comprises the following steps: establishing an ore deposit model, wherein the ore deposit model comprises mineralization geological elements, a mineralization process and a mineralization effect; obtaining mineralization prediction elements related to mineralization based on the ore deposit model; preprocessing the mineralization prediction elements; fuzzy processing is carried out on the preprocessed metallogenic prediction elements; determining training data based on the mineralization prediction elements after fuzzy processing; establishing a machine learning model; training the machine learning model based on the training data to obtain a trained machine learning model; and performing metallogenic prediction based on the trained machine learning model. According to the method provided by the invention, the prediction accuracy can be improved.

The invention relates to a quantitative detection method for a sulfur-containing compound in Baijiu. The method is characterized by comprising the following steps: (1) gathering volatile components inBaijiu; and (2) feeding the volatile components gathered in step (1) into a gas chromatography-sulfur chemiluminescence detector combined device, and quantitatively detecting the sulfur-containing compound. The sulfur chemiluminescence detector is used as the detector for sulfur-containing compound aromatic component of Baijiu and is used for quantitatively detecting the sulfur-containing compound, the selectivity and sensitivity for the sulfur-containing compound are high, the sulfur-containing compound with low content and low threshold value can be obtained, and the quantitative detectionmethod has instruction effect for the process such as blending, and quality inspection. The headspace solid-phase microextraction is combined with the gas chromatography-sulfur chemiluminescence detector technology to determine the content of the sulfur-containing compound in Baijiu. The quantitative detection method is simple in operation, high in result accuracy, good in stability, high in detection efficiency and capable of meeting the commercialization requirement of the mass detection of samples.

The invention discloses a method for improving the top-grafting survival rate of camellia trees. The method includes the steps of grafting time selection, rootstock selection, rootstock treatment, scion collection, grafting, first rootstock binding, second rootstock binding and fostering and fertilizing management. On sunny days from May to July every year, robust camellia tree branches on the camellia trees aged 20 to 30 years are selected as rootstocks; on the basis of a conventional bark-pulling cut-grafting method, it is unnecessary to keep vegetative branches and shading branches, film belts are used for binding the rootstocks from bottom to top one ring after another ring without gaps, and then the rootstocks are covered with moisturizing bags and shading objects; when scion buds survive, the moisturizing bags and the shading objects are removed, and then film belts are used for binding the rootstocks; after grafting, management is conducted in combination with a conventional method. By means of the method, rootstock germination can be effectively inhibited, it is prevented that the rootstocks are subjected to bark cracks and death due to sunburn and water loss of the rootstocks, the costs of management and protection after grafting and crown changing of the camellia trees are effectively reduced, and recovery and formation of crowns of the camellia trees after grafting can be quickened.

The invention relates to a casting polyurethane / diatomite composite material and a preparation method thereof. The composite material is prepared from the following raw materials: diatomite (modified by a silane coupling agent), polyurethane prepolymers and a chain extender, wherein the mass of the diatomite (modified by the silane coupling agent) accounts for 0.5-10% of that of the polyurethane prepolymer. The preparation method comprises the following steps: (1) at room temperature, carrying out modification on the diatomite by using a silane coupling agent so as to obtain modified diatomite; and (2) weighting and preheating polyurethane prepolymers, and adding the modified diatomite obtained in the step (1) into the preheated polyurethane prepolymers, mixing and stirring, then carrying out vacuum defoamation on the obtained object so as to obtain a mixture, after the chain extender is heated and molten, adding the molten chain extender into the mixture, mixing and stirring, casting the object into a mould to carry out primary vulcanization, after the primary vulcanization is completed, carrying out demoulding on the obtained object, and finally, carrying out secondary vulcanization on the obtained object. The CPU / diatomite composite material prepared in the invention is good in mechanical properties and heat resistance; and the preparation method disclosed by the invention is simple, direct, low in energy consumption, convenient for processing and forming, and suitable for being applied to industrial production.

The invention discloses an image color correction method for directly correcting RGB values by adopting a neural network, and belongs to the technical field of aerodynamic experimental measurement, and the method comprises the following steps: 1) establishing an image RGB color correction model based on a neural network algorithm; 2) taking the color calibration card as a reference color, and training parameters of a neural network color correction model; and 3) correcting the RGB value of the new image: taking the RGB value of the image shot by the camera as the input of the neural network, and obtaining the RGB value of the image after color correction at the output end of the neural network. According to the method disclosed by the invention, the problems of complex step sequence and information loss in a transformation process when an image color correction method is applied to an SSLC coating experimental measurement technology in the prior art are avoided. The method is simple and direct, is especially suitable for the SSLC coating measurement technology, and can also be used for other occasions requiring accurate color measurement.

The invention discloses a velocity field quick correction method and device based on a hydrodynamics continuity equation, and the device comprises a continuity equation discrete form module, a velocity field divergence calculation module, a derivation operator decomposition module, and a velocity field reconstruction module. The method achieves the direct calculation of a corrected velocity field through the eigenvalue decomposition of a one-dimensional derivation operator, and the obtained velocity field meets the continuity equation completely in a discrete form. The method achieves the most conservative correction of an original velocity field. The method and device can greatly improve the calculation efficiency, and reduce the consumption of a memory. The method is enabled to be higher in applicability.

The invention discloses Acipenser sinensis VTG protein antigen and antibody, and a preparation method and an application thereof. The preparation method comprises the following steps: 1, preparation of a recombinant Acipenser sinensis VTG protein antigen: 1) designing an upstream primer and a downstream primer, and amplifying a protein domain truncated sequence; 2) subjecting a pMD-18T-VTG plasmid and a Pet32a (+) expression vector to enzyme digestion by using EcoRI and XhoI, then purifying a target fragment, connecting the pMD-18T-VTG plasmid and the Pet32a (+) expression vector into Pet32a (+)-VTG, converting the Pet32a (+)-VTG into DH5a, coating the DH5a onto an ampicillin containing LB culture medium, and carrying out culturing; 3) converting the Pet32a (+)-VTG plasmid into a BL21(DE3) competent cell, coating the BL21(DE3) competent cell onto an ampicillin containing LB solid culture medium, and carrying out culturing; and 4) subjecting collected mycelium precipitate to resuspension precipitation by using an 8M urea containing protein buffer solution, then carrying out filtering, and collecting a supernatant so as to obtain a recombinant protein; 2, obtainment of an Acipenser sinensis VTG protein antibody through immunization of Japanese rabbit with the recombinant Acipenser sinensis VTG protein antigen; and 3, application of the Acipenser sinensis VTG protein antigen and antibody. The Acipenser sinensis VTG protein antibody and the VTG protein antigen provided by the invention have the following advantages: the immunity is good; a titer can reach 1: 1000; detection results have high positive rate; and the clinical applicability is good.

The invention discloses a false information early detection method based on a text structure algorithm, and belongs to the technical field of false information detection based on the text structure algorithm. The technical problem to be solved is to provide an improvement of a false information early detection method based on a text structure algorithm. According to the technical scheme, the method comprises the steps of document discourse unit obtaining, discourse unit representation learning and structure representation learning, a document discourse structure chart is constructed on the basis of a rhetortic structure theory, and global structure representation of discourse units is obtained through a multi-relational graph neural network; in context representation learning, the position adjacent relation of discourse units in a document serves as a calculation object, local context representations of the discourse units are obtained, all the discourse units of the document are fused to form document representations based on a gating recursion unit fused with a global attention mechanism, the generated document representations are used for false information detection, and a probability value of whether the input document belongs to false information or not is obtained. The method is applied to false information detection.

The invention provides a natural language analysis-based e-commerce user satisfaction degree evaluation method. The method comprises the following steps of (1) obtaining user message information in a target webpage through a crawler tool; (2) cleaning the user message information; (3) obtaining a single frequency degree and a repetition frequency degree of vocabularies in effective data of the user message information, and forming a product demand keyword set; (4) according to types and frequency degree values of keywords, obtaining product demand information; (5) obtaining production unit information; and (5) performing matching on the production unit information and the product demand information, and sending the corresponding product demand information to production units. The method has the beneficial effects that the product demand information of the market is extracted by performing natural language analysis on the user message information in a network and is sent to the corresponding production units, so that the resource matching degree is greatly increased and huge economic and social benefits are achieved; and the method is quick and direct, and a calculation result is high in reliability.

The invention provides a fluorescent cell model for screening an M2 ion channel blocker for influenza virus, and the cell model is a cell containing pH sensitive fluorescent materials. Meanwhile, the invention also provides a method for screening the M2 ion channel blocker for the influenza virus by the cell model, which is to detect the change of fluorescent values in the cell model under the condition of an acidic buffer. The invention has the advantages that the H+ flowing condition through the M2 ion channel is directly reflected by detecting the change of the fluorescent values reflecting pH in the cell; and the M2 ion channel blocker screening method established by the cell model has definite mechanism of drug action, is simple and convenient, quick and direct, and is suitable for high-flux accurate screening of the M2 ion channel blocker.

The invention discloses a method for analyzing and checking the local pattern density of a chip. The method comprises the following steps of: 1, defining the size of a local area according to chemical mechanical polarization (CMP) and the requirement of an etching process for macro loading, and dividing the chip into a plurality of local chip blocks of which the area is local area; 2, cutting each local chip block into a plurality of small equal-area local chip blocks; 3, checking the pattern density of each small local chip block; 4, drawing a two-dimensional contour chart of the pattern density of the chip; 5, calculating whether a region of which the area is larger than the local area and a pattern density value is beyond the density range of a target pattern exits in the two-dimensional contour chart; and 6, judging whether the design pattern is required to be changed according to a calculation value in the step 5. By the method, the checking capacity of the pattern density is enhanced without increase of false warning cases during checking of the pattern density.

The invention belongs to the chemical cellulose technical field, and discloses a method and an apparatus for evaluating cellulose pulp reaction performance. The method for evaluating the cellulose pulp reaction performance comprises the following steps: adding a reaction reagent, a solvent and a catalyst in a reaction vessel; setting a reaction temperature Tr, controlling a material and a jacket medium in the reaction vessel to reach the reaction temperature Tr, wherein Tr equals Tj, the Tj is the jacket temperature; measuring the specific heat and heat transfer coefficient of the reaction material under the temperature, adding the cellulose pulp with uniform speed by an invariant feeding rate to react, simultaneously measuring Tr and Tj; measuring and collecting the viscosity and turbidity data, when the absolute value of Tr and Tj is less than or equal to 0.1 DEG C, completing the reaction, measuring the specific heat and heat transfer coefficient of a mixed system in the reaction vessel, calculating the reaction heat release rate Qr, and calculating to obtain the enthalpy delta Hr. The invention comprehensively use the reaction calorimetry, the turbidity and the viscosity for measuring.

The invention discloses a method for improving the reproductive performance of caged small-medium-sized meat breeding ducks. After the hatching and during a rearing period, unqualified breeding ducksare eliminated in time, the appearance characteristics of male ducks that meet the breed requirements and are moderate in weight and healthy and strong are selected, and then through the screening ofbreeding by one-to-one, male and female ducks different rotation methods and different ratios, the male ducks with strong breeding ability, a breeding method with high fertilization rate and a suitable male-female ratio are selected. The problem of difficult breeding of the caged small-medium-sized meat breeding ducks is effectively solved. The unqualified reserve breeding ducks are eliminated intime after hatching and in the rearing period, only the best quality male ducks are reserved for breeding, unnecessary breeding of redundant male ducks is avoided, the breeding cost is reduced, the method is simple and easy to operate, and the reproductive efficiency of the caged meat breeding ducks is high.

The invention discloses a telescope secondary mirror position correcting method based on optical spot definition function. The method comprises the following steps of 1, obtaining the position of a secondary mirror with objective function having an extreme value by taking onaxis field-of-view optical spot definition as the objective function, translation of the secondary mirror along the direction of an optical axis and translation and rotation of the secondary mirror along two orthogonal directions vertical to the optical axis direction through an iterative algorithm, wherein if all the variables are simultaneously iterated, the objective function is likely to be converged to a local extreme value, so that each iterative process is enabled to comprise two steps of iteration of the optical axis direction and the direction vertical to the optical axis direction so as to enable the objective function to be converged to a global extreme value; 2, enabling the secondary mirror to rotate around a zero comet difference point of a system along the two orthogonal directions and searching for the extreme value of the objective function in an iterative manner for realizing correction for the relative position of the secondary mirror by taking the mean value of definition function of multiple axis external field-of-view optical spots as the objective function. The method is free from wavefront detection and reestablishment, adjustment for a telescope system has an objective standard, and the telescope imaging quality can be monitored in real time.

The invention discloses a method for screening a single-copy T-DNA transgenic plant. The method comprises the following steps: forming a sandwich relationship (R1-T-R2) in a double-copy transgenic plant through a pair of reference amplified fragments (R1 and R2) in a plant genome and a Ct value amplified by T-DNA (T); and enabling the Ct value of the double-copy T-DNA transgenic plant to be between R1 and R2, and forming a sandwich type R1-T-R2 sandwich Ct relationship. Compared with a double-copy plant, the T-DNA amplification Ct value of the single-copy plant is increased by about 1, and the Ct value of the single-copy plant is shifted out of the sandwich relationship, so that a Ct mode of R1-R2-T is generated; and a single-copy T-DNA transgenic plant is found out by comparing different Ct value relationships. The method does not need to calculate [delta]Ct, [delta][delta]Ct and 2-[delta][delta]Ct, the change of the transgenic copy number is judged by directly displaying the relationship between the two pairs of reference sequences (R1 and R2) and the amplification Ct value of T-DNA, and the method is direct, simple and reliable.

The invention discloses a method for analyzing and checking the local pattern density of a chip. The method comprises the following steps of: 1, defining the size of a local area according to chemical mechanical polarization (CMP) and the requirement of an etching process for macro loading, and dividing the chip into a plurality of local chip blocks of which the area is local area; 2, cutting each local chip block into a plurality of small equal-area local chip blocks; 3, checking the pattern density of each small local chip block; 4, drawing a two-dimensional contour chart of the pattern density of the chip; 5, calculating whether a region of which the area is larger than the local area and a pattern density value is beyond the density range of a target pattern exits in the two-dimensional contour chart; and 6, judging whether the design pattern is required to be changed according to a calculation value in the step 5. By the method, the checking capacity of the pattern density is enhanced without increase of false warning cases during checking of the pattern density.

The invention discloses a method for detecting an organic carbon-water distribution coefficient of aromatic hydrocarbon in actual soil and belongs to the technical field of environmental risk evaluation and measurement. The method for detecting the organic carbon-water distribution coefficient of the aromatic hydrocarbon in the actual soil specifically comprises the steps of detecting the concentration (C1) of an aromatic hydrocarbon compound absorbed to a passive sampler adsorbing material in an equilibrium state to predicate a freely dissolved concentration (Cfree) in a soil liquid phase onthe basis of an equilibrium distribution theory and combining the concentration (Cs) in the soil and an organic carbon content (foc) in the soil to calculate a Koc value. The method for detecting theorganic carbon-water distribution coefficient of the aromatic hydrocarbon in the actual soil, disclosed by the invention, has the advantages that the sensitivity is high; the operation is simple; requirements on data information are relatively low; the applicability is good; problems that the fluctuations among different models are great, the reproducibility is poor and the Kow-Koc correlation model method is not suitable for high hydrophobic organic compounds by using the Kow-Koc correlation model method are solved; problems that the influences of dissolved organic matters in the soil on a traditional actual detecting method are great and the Koc value can be obviously undervalued are solved; and the method is of great significance to objectively evaluate migration and transformation of the aromatic hydrocarbon compound in the soil and harms of the aromatic hydrocarbon compound to physical health.

The invention discloses a method for preparing a magnetic copper ion imprinting material from steel pickling waste liquid and application of the method. The method comprises the following steps: adding the steel pickling waste liquid into a chitosan acid solution, and uniformly mixing; adjusting the pH to 9-11, heating to react to generate black precipitate, separating the black precipitate out and putting the black precipitate into a copper salt solution, and adding a cross-linking agent for cross-linking reaction; washing the product to be neutral, and eluting the product with the acid solution until no copper ion is contained; performing magnetic separation to obtain the magnetic product namely the magnetic copper ion imprinting material. With adoption of the method, high efficiency and simpleness are realized, high energy consumption instruments such as an ultrasonic apparatus are not needed; the obtained magnetic copper ion imprinting material has high specific adsorbability to copper ions, can be recycled, is stable in chemical properties, does not release toxic substances, and is free of secondary pollution; the water liquid serves as an iron source, so that the waste material is turned into things of value, the cost is decreased, and the pollution is reduced. An excellent application prospect is realized for comprehensive utilization of steel pickling water liquid and treatment of copper-containing wastewater, and huge environmental and economical benefits are realized.

The invention relates to the technical field of oilfield development, and discloses a method and device for evaluating the contribution degree of chemical system in-situ emulsification to improvementof the crude oil recovery ratio. The method comprises the steps that (I) a first core model and a second core model are vacuumized and then injected into formation water, then the formation water is driven by oil, and the saturated oil mass Vo1 of a second core is calculated; (II) after the first core is driven by water, produced liquid is obtained through driving by a chemical system; (III) the second core is driven by the produced liquid, and the oil displacement Vo3 after emulsification of the chemical system is calculated; (IV) the steps (I) - (II) are repeated, and the saturated oil massVo2 of the second core is calculated; and (V) the produced liquid of the first core is subjected to demulsification treatment and then drives the second core, and the oil displacement Vo4 of non-emulsification of the chemical system is calculated. The contribution degree of chemical system in-situ emulsification to improvement of the crude oil recovery ratio is calculated according to the formula(a), wherein W = (Vo3 / Vo 1-Vo 4 / Vo2) * %; and formula (a) sees the description.

The invention discloses a white spirit cold-soaking and countercurrent extraction method of polygonatum odoratum polysaccharide in a brewing technique and an online quality control method thereof. The extraction method comprises the steps of (1) slicing polygonatum odoratum or cutting the polygonatum odoratum into sections or grinding the polygonatum odoratum into granules; (2) cold-soaking the polygonatum odoratum in white spirit with alcohol concentration-volume ratio being 45-55% according to a charge rate of 1: (7.0-8.0) by mass; and (3) implementing countercurrent extraction by using white spirit of which the alcohol concentration-volume ratio is 45-55%. According to the optimized solution, the polygonatum odoratum is sliced or cut into sections or ground into granules and is pressed flat by external force. The method disclosed by the invention is direct and high in efficiency, overcomes difficulty in filtration, avoids contamination from moulds and can prevent polysaccharide which has relatively large molecular weight from precipitating in healthcare wine, thus being an ideal method for extracting polygonatum odoratum in the white spirit brewing technique. The solution of the control method does not need pre-treatment on a sample, comprises few analysis step and generates few error influence factors, consumes little time, and is capable of meeting demands of rapid determination and online quality control of samples in batches.

The invention discloses a preparation method of a trifluoromethylated polypeptide compound shown as a formula (II). The method comprises the following steps: by using a mixed solvent of DMF and waterin a volume ratio of 1:(1-4) as a reaction medium, reacting a polypeptide compound shown as a formula (I) with 1-(trifluoromethyl)-1,2-benziodoxol-3(1H)-one in the presence of a photocatalyst at roomtemperature under blue light for 3-8 hours, and after the reaction is completed, carrying out post-treatment on the obtained reaction mixture to obtain the trifluoromethylated polypeptide compound asshown in the formula (II). According to the method, the reaction is driven to be carried out under the irradiation of visible light, the reaction solvent is a mixed solvent of DMF and water, the greenchemical concept is better met, the product can be prepared in one step, the operation process is simple, raw material sources are commercialized and easy to obtain, the photocatalyst is utilized, good catalytic performance is achieved, the reaction condition is mild, and the site selectivity is high.

The invention belongs to the technical field of organic chemistry, and particularly relates to a method for preparing an azide compound through azidation based on C-H activated anilines. The method comprises the following steps: by using anhydrous copper acetate as a reaction catalyst and hydrogen peroxide as an oxidant, adding an aniline compound and sodium azide into water, reacting for two hours at a room temperature, extracting by using ethyl acetate, and carrying out vacuum concentration, thus obtaining a corresponding organic azide coarse product; carrying out column chromatography separation and purification, thus obtaining a corresponding pure product. The method disclosed by the invention has the characteristics that the operation is simple, the reaction conditions are gentle, the reaction time is short, and the method is green and environment-friendly.

The invention discloses a white spirit cold-soaking and countercurrent extraction method of polygonatum odoratum polysaccharide in a brewing technique and an online quality control method thereof. The extraction method comprises the steps of (1) slicing polygonatum odoratum or cutting the polygonatum odoratum into sections or grinding the polygonatum odoratum into granules; (2) cold-soaking the polygonatum odoratum in white spirit with alcohol concentration-volume ratio being 45-55% according to a charge rate of 1: (7.0-8.0) by mass; and (3) implementing countercurrent extraction by using white spirit of which the alcohol concentration-volume ratio is 45-55%. According to the optimized solution, the polygonatum odoratum is sliced or cut into sections or ground into granules and is pressed flat by external force. The method disclosed by the invention is direct and high in efficiency, overcomes difficulty in filtration, avoids contamination from moulds and can prevent polysaccharide which has relatively large molecular weight from precipitating in healthcare wine, thus being an ideal method for extracting polygonatum odoratum in the white spirit brewing technique. The solution of the control method does not need pre-treatment on a sample, comprises few analysis step and generates few error influence factors, consumes little time, and is capable of meeting demands of rapid determination and online quality control of samples in batches.