49results about How to "Method directly" patented technology

The invention provides a temporomandibular joint movement reconstruction method and a system thereof. The method is characterized by collecting an image sequence of a mark point track of open and close mouth mandible movements of a photographed person; determining three-dimensional coordinates of all the mark points on an intraoral fixed mark device in each frame of image, acquiring a movement track of each mark point in a space, and collecting a CBCT image of the intraoral fixed mark device and collecting a CBCT image of the photographed person; registering the mark point of the CBCT image of the intraoral fixed mark device with the mark point acquired by a camera through photographing, and acquiring a transformation matrix of a mark point movement; and using the transformation matrix to carry out rigid body transformation on the CBCT image of the photographed person, acquiring a CBCT image sequence of the photographed person and acquiring a temporomandibular movement image. In the invention, real movement conditions of mandible condyle process and a temporal bone joint surface of a human-body temporomandibular joint can be acquired, the method and the system can be used for auxiliary diagnosis of a temporomandibular joint disease and dynamic analysis of joint motion, temporomandibular joint diagnosis means are increased, doctor diagnosis accuracy and efficiency can be increased, and medical quality and efficiency can be increased too.

The invention discloses an avalanche photodiode (APD) gain stabilization control device and an APD gain stabilization control method. The device consists of a bias control circuit, an APD detection circuit, a data acquisition card and a computer; and the control method comprises the following steps that: the computer directly judges difference of an average digital signal acquired by the data acquisition card and a set signal value and transmits an APD bias regulating command, and the APD bias voltage is fed back and controlled through the bias control circuit; the whole device forms a closed loop feedback system; and the APD amplifier gain is finely adjusted so as to fulfill the aim of stabilizing the acquired digital signal. The invention has the characteristics that: the complexity and unreliability of a method for controlling APD temperature to be constant or a temperature-based feedback bias control method are avoided, the stability and reliability of the acquired digital signal can be easily and effectively improved, and the method is direct, effective, simple and reliable and has high practical and commercial values.

The invention discloses a method for predicting maximum height of a filling mining diversion fissure zone. Supposing a precondition that no caving zone is generated and only a fissure zone and a sagging zone are generated in the advancing process of a working face, a mathematical model which is used for predicting the maximal height of the filling mining diversion fissure zone; a critical value of top board deformation is used for determining the strata failure low of an over-limit stress limited space; and whether the strata fails is determined according to the critical value of the top board deformation. Therefore, compared with the prior art, the method has advantages of more direct calculation and higher convenience. A calculation formula for predicting the maximum height of the filling mining diversion fissure zone can be simply and accurately obtained. Furthermore the parameters in the formula can be easily acquired, and higher accuracy of the calculation process can be realized. Simple and direct calculation can be obtained. The method can quickly, effectively and accurately predict the maximum height of the diversion fissure zone and is suitable for guidance for actual production, thereby realizing safe mining under water body.

The invention relates to a casting polyurethane/diatomite composite material and a preparation method thereof. The composite material is prepared from the following raw materials: diatomite (modified by a silane coupling agent), polyurethane prepolymers and a chain extender, wherein the mass of the diatomite (modified by the silane coupling agent) accounts for 0.5-10% of that of the polyurethane prepolymer. The preparation method comprises the following steps: (1) at room temperature, carrying out modification on the diatomite by using a silane coupling agent so as to obtain modified diatomite; and (2) weighting and preheating polyurethane prepolymers, and adding the modified diatomite obtained in the step (1) into the preheated polyurethane prepolymers, mixing and stirring, then carrying out vacuum defoamation on the obtained object so as to obtain a mixture, after the chain extender is heated and molten, adding the molten chain extender into the mixture, mixing and stirring, casting the object into a mould to carry out primary vulcanization, after the primary vulcanization is completed, carrying out demoulding on the obtained object, and finally, carrying out secondary vulcanization on the obtained object. The CPU/diatomite composite material prepared in the invention is good in mechanical properties and heat resistance; and the preparation method disclosed by the invention is simple, direct, low in energy consumption, convenient for processing and forming, and suitable for being applied to industrial production.

The invention discloses an image color correction method for directly correcting RGB values by adopting a neural network, and belongs to the technical field of aerodynamic experimental measurement, and the method comprises the following steps: 1) establishing an image RGB color correction model based on a neural network algorithm; 2) taking the color calibration card as a reference color, and training parameters of a neural network color correction model; and 3) correcting the RGB value of the new image: taking the RGB value of the image shot by the camera as the input of the neural network, and obtaining the RGB value of the image after color correction at the output end of the neural network. According to the method disclosed by the invention, the problems of complex step sequence and information loss in a transformation process when an image color correction method is applied to an SSLC coating experimental measurement technology in the prior art are avoided. The method is simple and direct, is especially suitable for the SSLC coating measurement technology, and can also be used for other occasions requiring accurate color measurement.

The invention discloses Acipenser sinensis VTG protein antigen and antibody, and a preparation method and an application thereof. The preparation method comprises the following steps: 1, preparation of a recombinant Acipenser sinensis VTG protein antigen: 1) designing an upstream primer and a downstream primer, and amplifying a protein domain truncated sequence; 2) subjecting a pMD-18T-VTG plasmid and a Pet32a (+) expression vector to enzyme digestion by using EcoRI and XhoI, then purifying a target fragment, connecting the pMD-18T-VTG plasmid and the Pet32a (+) expression vector into Pet32a (+)-VTG, converting the Pet32a (+)-VTG into DH5a, coating the DH5a onto an ampicillin containing LB culture medium, and carrying out culturing; 3) converting the Pet32a (+)-VTG plasmid into a BL21(DE3) competent cell, coating the BL21(DE3) competent cell onto an ampicillin containing LB solid culture medium, and carrying out culturing; and 4) subjecting collected mycelium precipitate to resuspension precipitation by using an 8M urea containing protein buffer solution, then carrying out filtering, and collecting a supernatant so as to obtain a recombinant protein; 2, obtainment of an Acipenser sinensis VTG protein antibody through immunization of Japanese rabbit with the recombinant Acipenser sinensis VTG protein antigen; and 3, application of the Acipenser sinensis VTG protein antigen and antibody. The Acipenser sinensis VTG protein antibody and the VTG protein antigen provided by the invention have the following advantages: the immunity is good; a titer can reach 1: 1000; detection results have high positive rate; and the clinical applicability is good.

The invention discloses a false information early detection method based on a text structure algorithm, and belongs to the technical field of false information detection based on the text structure algorithm. The technical problem to be solved is to provide an improvement of a false information early detection method based on a text structure algorithm. According to the technical scheme, the method comprises the steps of document discourse unit obtaining, discourse unit representation learning and structure representation learning, a document discourse structure chart is constructed on the basis of a rhetortic structure theory, and global structure representation of discourse units is obtained through a multi-relational graph neural network; in context representation learning, the position adjacent relation of discourse units in a document serves as a calculation object, local context representations of the discourse units are obtained, all the discourse units of the document are fused to form document representations based on a gating recursion unit fused with a global attention mechanism, the generated document representations are used for false information detection, and a probability value of whether the input document belongs to false information or not is obtained. The method is applied to false information detection.

The invention provides a fluorescent cell model for screening an M2 ion channel blocker for influenza virus, and the cell model is a cell containing pH sensitive fluorescent materials. Meanwhile, the invention also provides a method for screening the M2 ion channel blocker for the influenza virus by the cell model, which is to detect the change of fluorescent values in the cell model under the condition of an acidic buffer. The invention has the advantages that the H+ flowing condition through the M2 ion channel is directly reflected by detecting the change of the fluorescent values reflecting pH in the cell; and the M2 ion channel blocker screening method established by the cell model has definite mechanism of drug action, is simple and convenient, quick and direct, and is suitable for high-flux accurate screening of the M2 ion channel blocker.

The invention belongs to the chemical cellulose technical field, and discloses a method and an apparatus for evaluating cellulose pulp reaction performance. The method for evaluating the cellulose pulp reaction performance comprises the following steps: adding a reaction reagent, a solvent and a catalyst in a reaction vessel; setting a reaction temperature Tr, controlling a material and a jacket medium in the reaction vessel to reach the reaction temperature Tr, wherein Tr equals Tj, the Tj is the jacket temperature; measuring the specific heat and heat transfer coefficient of the reaction material under the temperature, adding the cellulose pulp with uniform speed by an invariant feeding rate to react, simultaneously measuring Tr and Tj; measuring and collecting the viscosity and turbidity data, when the absolute value of Tr and Tj is less than or equal to 0.1 DEG C, completing the reaction, measuring the specific heat and heat transfer coefficient of a mixed system in the reaction vessel, calculating the reaction heat release rate Qr, and calculating to obtain the enthalpy delta Hr. The invention comprehensively use the reaction calorimetry, the turbidity and the viscosity for measuring.

The invention discloses a method for improving the reproductive performance of caged small-medium-sized meat breeding ducks. After the hatching and during a rearing period, unqualified breeding ducksare eliminated in time, the appearance characteristics of male ducks that meet the breed requirements and are moderate in weight and healthy and strong are selected, and then through the screening ofbreeding by one-to-one, male and female ducks different rotation methods and different ratios, the male ducks with strong breeding ability, a breeding method with high fertilization rate and a suitable male-female ratio are selected. The problem of difficult breeding of the caged small-medium-sized meat breeding ducks is effectively solved. The unqualified reserve breeding ducks are eliminated intime after hatching and in the rearing period, only the best quality male ducks are reserved for breeding, unnecessary breeding of redundant male ducks is avoided, the breeding cost is reduced, the method is simple and easy to operate, and the reproductive efficiency of the caged meat breeding ducks is high.

The invention discloses a method for screening a single-copy T-DNA transgenic plant. The method comprises the following steps: forming a sandwich relationship (R1-T-R2) in a double-copy transgenic plant through a pair of reference amplified fragments (R1 and R2) in a plant genome and a Ct value amplified by T-DNA (T); and enabling the Ct value of the double-copy T-DNA transgenic plant to be between R1 and R2, and forming a sandwich type R1-T-R2 sandwich Ct relationship. Compared with a double-copy plant, the T-DNA amplification Ct value of the single-copy plant is increased by about 1, and the Ct value of the single-copy plant is shifted out of the sandwich relationship, so that a Ct mode of R1-R2-T is generated; and a single-copy T-DNA transgenic plant is found out by comparing different Ct value relationships. The method does not need to calculate [delta]Ct, [delta][delta]Ct and 2-[delta][delta]Ct, the change of the transgenic copy number is judged by directly displaying the relationship between the two pairs of reference sequences (R1 and R2) and the amplification Ct value of T-DNA, and the method is direct, simple and reliable.

The invention discloses a preparation method of a trifluoromethylated polypeptide compound shown as a formula (II). The method comprises the following steps: by using a mixed solvent of DMF and waterin a volume ratio of 1:(1-4) as a reaction medium, reacting a polypeptide compound shown as a formula (I) with 1-(trifluoromethyl)-1,2-benziodoxol-3(1H)-one in the presence of a photocatalyst at roomtemperature under blue light for 3-8 hours, and after the reaction is completed, carrying out post-treatment on the obtained reaction mixture to obtain the trifluoromethylated polypeptide compound asshown in the formula (II). According to the method, the reaction is driven to be carried out under the irradiation of visible light, the reaction solvent is a mixed solvent of DMF and water, the greenchemical concept is better met, the product can be prepared in one step, the operation process is simple, raw material sources are commercialized and easy to obtain, the photocatalyst is utilized, good catalytic performance is achieved, the reaction condition is mild, and the site selectivity is high.

The invention belongs to the technical field of organic chemistry, and particularly relates to a method for preparing an azide compound through azidation based on C-H activated anilines. The method comprises the following steps: by using anhydrous copper acetate as a reaction catalyst and hydrogen peroxide as an oxidant, adding an aniline compound and sodium azide into water, reacting for two hours at a room temperature, extracting by using ethyl acetate, and carrying out vacuum concentration, thus obtaining a corresponding organic azide coarse product; carrying out column chromatography separation and purification, thus obtaining a corresponding pure product. The method disclosed by the invention has the characteristics that the operation is simple, the reaction conditions are gentle, the reaction time is short, and the method is green and environment-friendly.