The invention discloses an acidic corrosion-resistant X65 seamless steel pipeline and a manufacturing method thereof. The acidic corrosion-resistant X65 seamless steel pipeline comprises, by weight, 0.08-0.16% of C, 0.15-0.35% of Si, 1.0-1.6% of Mn, 0.02-0.05% of Al, 0.03-0.049% of V, 0.01-0.04% of Ti, 0.20-0.30% of Cu, 0.20-0.30% of Ni, less than or equal to 0.015% of P and less than or equal to 0.005% of S. When C content is less than or equal to 0.12%, CE<Pcm> is less than or equal to 0.25 and when C content is greater than 0.12%, CE<IIW> is less than or equal to 0.42. According to the manufacturing method of the acidic corrosion-resistant X65 seamless steel pipeline, a continuous rolling method is used, a pipe blank is heated to a temperature of 1200-1300 DEG C through an annular furnace, a tube knockout temperature is in a range of 1190-1240 DEG C and a sizing temperature is in a range of 820-850 DEG C, in quenching and tempering treatment, a quenching heating temperature is in a range of 890-910 DEG C, a quenching medium is water, thermal insulation time is greater than or equal to 30min, a tempering temperature is in a range of 580-620 DEG C, thermal insulation time is greater than or equal to 50min, and heating straightening and flaw detection examination are carried out at a temperature greater than or equal to 400 DEG C.