The invention discloses a high-pressure decorative door sheet comprising wood base materials and a preparation method of the door sheet. The door sheet comprises base plate layers, buffer layers, decorative layers and wear-resistant protective layers which are sequentially arranged from bottom to top, wherein the base plate layer comprises at least two wood base material layers and impregnated surface paper layers for bonding are arranged between the wood base material layers. The plate layers are sequentially stacked in an intermittent hot press in the order of one wear-resistant protection layer, one decorative layer, one buffer layer, one impregnated surface paper layer for bonding, one base plate layer, a polypropylene isolating membrane, the other base plate layer, the other impregnated surface paper layer for bonding, the other buffer layer, the other decorative layer and the other wear-resistant protection layer and then subjected to high-temperature and high-pressure one-time hot press molding. The high-pressure decorative door sheet comprising the wood base materials is flame-retardant, fireproof, wear-resistant, scratch-resistant, waterproof, damp-proof, impact-resistant, fouling-resistant and scrape-resistant, the decorative layers and the base materials are subjected to one-time hot press molding and can be used without pasting, decorative surface layers cannot peel off and mildew, the surface is easy to clean, the formaldehyde release amount and TVOC release amount are low, and no heavy metals are contained.