The invention discloses a closed-loop sampling system for flow-cell-process drug dissolution and a use method thereof. The closed-loop sampling system comprises a constant-temperature water area system, a flow cell system, a lifting mechanism, a circulating heating system, a solvent monitoring system, a solvent circulating system and a sampling system, wherein the constant-temperature water area system comprises a constant-temperature water tank rack, a constant-temperature water tank, a solvent cup holder, a solvent cup and a solvent cup cover; the flow cell system is arranged at the top endof the constant-temperature water area system; the lifting mechanism is arranged on one side of the constant-temperature water tank rack; the circulating heating system comprises a heating device anda water circulating pump; the circulating inlet end of the solvent monitoring system communicates with the heating device, and the circulating outlet end of the solvent monitoring system respectivelycommunicates with the solvent cup and a flow cell; the solvent circulating system comprises a solvent circulating pump and a pulse valve; and the sampling system comprises a sampling mechanism, a sampling pipeline, a sampling valve and a sampling pump. The system is compact in structure, the heating efficiency of the heating device is high, water area temperature distribution is uniform, water temperature control is accurate, and temperature and pressure of a solvent are monitored.