The invention provides a lyophilized collagen mask. The lyophilized collagen mask is prepared from recombinant human-derived collagen, sodium hyaluronate, betaine, hydroxyethyl cellulose, a first nutrient, a second nutrient, a third nutrient, a lyophilizing adjuvant and deionized water. By reasonably proportioning collagen, nutrient substances and other components, the lyophilized collagen refreshing mask which is convenient to store, not easy to deteriorate, high in activity and easy to use can be prepared and can be used immediately after being dissolved, so that the safety of the raw materials can be kept, and the use convenience is improved. Hydroxyethylurea, tetrahydromethylpyrimidine carboxylic acid and nicotinamide are added into the formula, so that the stable state of human skin can be maintained, the epidermal repair speed is accelerated, and wound healing is promoted. Compared with the formula in the prior art, the product has the advantages of high safety, low irritation risk, rich composition, high effectiveness, high security, high bioactivity, good moisturizing effect, human body absorption improvement, epidermal repair speed acceleration and wound healing promotion.