The invention relates to lychee rose wine as well as a preparation method and an application thereof. According to the lychee rose wine, lychee base wine, which is prepared by conducting scientific fermentation on lychee, is blended with red wine and white wine, and with the lychee serving as a monarch which is added with finely selected fruit of Chinese wolfberry, red jujube, radix glycyrrhizae,radix ginseng and apple powder and is assisted by a blending agent and a proper amount of adjuvants, the lychee rose wine is prepared by the method comprising the following steps: 1) harvesting, sorting, selecting and cleaning, cooling, peeling off and depitting, and squeezing the lychee, and conducting enzymatic hydrolysis; 2) standing by and conducting clarifying, separating clear juice, freezing and concentrating the clear juice, adjusting components of the juice, conducting fermentation in a tank, ending the fermentation, conducting aging, implementing fining and implementing cold treatment, so that the lychee base wine is obtained; 3) adding the fruit of Chinese wolfberry, the red jujube, the radix glycyrrhizae, the radix ginseng and the apple powder; and 4) conducting blending, filtering obtained liquid by virtue of a membrane, and filling and sealing the liquid, so that the lychee rose wine is obtained. The lychee rose wine prepared by the invention is clear and transparent in wine body and low in content of volatile acid, and the lingering bitterness of the wine body is obviously reduced and the wine body is good in taste; the wine is low in content of fusel oil; and the wine has such functions of resisting oxidation and the like, and the wine is suitable for tonifying qi and blood, promoting blood circulation and removing toxins. The lychee rose wine provided by the invention, which is applicable to preparation of various health-care wine drinks, has a broad market prospect.