The invention belongs to the technical field of textile materials, and particularly relates to a Taxus chinensis environment-friendly antibacterial anti-mite wadding and a preparation method thereof.The Taxus chinensis wadding provided by the invention comprises, by mass, 15-60% of Taxus chinensis viscose fibers and 40-85% of a fiber mixture, wherein the fiber mixture includes two or more of cotton fibers, composite antibacterial agent finished viscose fibers, natural mineral fibers, modal fibers, heat-melting silk polyester fibers or wool fibers. The steps of material mixing, air suction, carding, laying, hot melting, post-ironing, cooling, cutting and the like are performed to obtain the prepared Taxus chinensis wadding having multiple layers, good fluffy property, strong heat retentionproperty and good resilience, having the functions of bacteria inhibiting, mite preventing, moisture absorbing, sweat discharging, natural health care and static electricity preventing, having the characteristics of smoothness, fineness, coolness and the like, having a functional health care effect, and being beneficial to promoting sleep.