The invention discloses a preparation method of a luminous adhesive. The preparation method comprises 1, taking epoxy resin, wherein when a room temperature is lower than 20 DEG C, the epoxy resin has large viscosity and is inconvenient for adhesive blending and homogenization and thus the epoxy resin needs to be heated in a water bath before use, 2, adding 50-120% of phosphor into the epoxy resin, carrying out stirring by a stirrer for 30min until the phosphor is completely dissolved in the epoxy resin, 3, adding 100% of 650 polyamide resin (as a plasticizer) into the epoxy resin mixture, carrying out stirring to obtain a uniform mixture, adding a proper amount of a filler into the mixture and carrying out stirring to obtain a uniform mixture, and 4, slowly adding an amine curing agent into the mixture and carrying out curing at a room temperature for 24h to obtain the luminous adhesive. The luminous adhesive has luminescence, water resistance, durability and good stability, can be realized easily, is convenient for operation, and has a low production cost.