The invention discloses vagina health care gel as well as a preparation method and application thereof. The invention discloses the vagina health care gel for the first time. The vagina health care gel preservative comprises water, hydroxyethyl cellulose, inulin, water-soluble chitosan, polylysine, trehalose, arginine, disodium EDTA, glycerine, potassium alum, hydroxyacetophenone, tremella polysaccharide, crocus sativus flower oil, sesame seed oil, levulinic acid/sodium levulinate, a propolis extract, a licorice root extract, a purslane herb extract, a houttuynia cordata extract, WS23, lacticacid and a lactobacillus fermentation product. The vaginal care gel provided by the invention can be used for preparing vaginal care disinfection hygienic products, personal care products or medical instruments. The gel disclosed by the invention can be used for rapidly relieving discomfort such as vaginal pain and itching, dryness and the like, removing peculiar smell and balancing vaginal floraand pH value, and can be used for repairing, tightening and strengthening the immune defense function of vaginal mucosa after being used for a long time. The gel adopts an additive-free formula, the raw materials are all food-grade, and the gel is non-irritant to mucous membranes and skin, mild and safe.