The invention relates to a method and equipment for producing bio-butanol. The equipment comprises four production units, namely fermentation substrate preparation, fermentation, solvent rectification and waste mash treatment, wherein the rectification unit comprises beer columns, an ethylene-propylene tower, a butanol tower, an acetone tower, an aldehyde-removing tower and an ethanol tower, and comprises the following process flow: mature mash feed is delivered into a beer column I, is extracted from the side of the beer column I to enter a beer column II, and crude solvents with water content of between 30 and 80 percent extracted from the top of the beer column I and the beer column II are both delivered to the ethylene-propylene tower to be further rectified; the material is produced into crude butanol and mixture of acetone, ethanol and water through the ethylene-propylene tower; the crude butanol is refined into a butanol finished product through the butanol tower; the mixture of acetone, ethanol and water is produced into crude acetone and crude ethanol through the acetone tower; the crude acetone is refined into an acetone finished product through the aldehyde-removing tower; and the crude ethanol is refined into an ethanol finished product through the ethanol tower. The method and the equipment can effectively reduce material consumption and energy consumption in the process for producing the bio-butanol, reduce quantity of wastewater effluent in the production process, and improve market competitiveness of the bio-butanol product.