The invention discloses eco-friendly anti-wear lubricating oil, which is made from the following components: modified base oil, succinate, a cleanup agent, octyl diphenylamine, an antifoaming agent, an anti-friction and anti-wear synergist, dodecanedioic acid, beta-sitosterol, cholesteryl nonanoate, castor oil and auxiliary additives. The lubricating oil is excellent in antioxidant corrosion resistance, anti-rust property, anti-wear property, anti-friction property, bearing property, corrosion resistance and emulsion resistance, and is high in torque, long in oil change period, reduced in waste oil, and low in price when compared with conventional lubricating oil. The lubricating oil is good in chemical stability and lubricating property and excellent in high and low temperature resistance, can be totally biodegraded and is free of toxicity. The lubricating oil causes no effect on operators' health during research and development, production and use processes, has no effect on the environment, and is a green product conforming to environmental requirements. The lubricating oil is low in production cost, simple in preparation technology, and suitable for industrial production.