The invention discloses a plant cultivation medium and a preparation method thereof. The plant cultivation medium includes paper pulp, an organic fertilizer and a preservative agent; and the weight ratio of the paper pulp, the organic fertilizer and the preservative agent is the paper pulp: the organic fertilizer: the preservative agent= 100: 1-50: 0.05-0.5. Compared with the prior art, the preparation method has the following beneficial effects: 1), the plant cultivation medium can keep water in long term, can avoid waterlogging, and can facilitate the growth of plants; 2), the paper pulp covers the organic fertilizer, which can ensure continuous growth of the plants; 3), the preservative agent can prevent the plant cultivation medium from decomposition, and ensure the healthy growth of roots of the plants; 4) the paper pulp has high plasticity, various colors and shapes can be set, and the ornamental value is high; 5) drying treatment is performed on the paper pulp, the dried paper pulp is light, is clean, and is easy to use and transport; and 6), the plant cultivation medium is easy to prepare and is low in cost, can be recycled, and has a wide market prospect in the field of potted plants.