The invention relates to a large area super-flat reinforced concrete floor and colorful diamond sand surface layer one-step forming method. The one-step forming method comprises the steps of template erection, steel bar binding, expansion reinforcing band arrangement, elevation measurement, steel bar elevation check, laser leveler adjustment, concrete pouring, laser leveler leveling, professional leveling, diamond sand spreading, grinding and flexible compacting, polishing, construction seam cutting, curing, finished product curing and upper structure construction. According to the one-step forming method, a structural floor is used as a base layer, a diamond sand floor surface layer and a structure are subjected to one-step pouring forming, the common quality problem that the floor surface layer is prone to forming hollow protrusions due to secondary pouring is avoided, meanwhile, one concrete base layer is reduced, and cost is greatly reduced. The floor is fully covered with recyclable materials such as plastic films and templates so that an upper main structure can continue to be conveniently constructed.